Multi-Modal Fluorescence Imaging Flow Cytometry System
US-2024353309-A1 · Oct 24, 2024 · US
US2018329191A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2018329191-A1 |
| Application number | US-201715670683-A |
| Country | US |
| Kind code | A1 |
| Filing date | Aug 7, 2017 |
| Priority date | May 11, 2017 |
| Publication date | Nov 15, 2018 |
| Grant date | — |
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A method for ultrafast time-resolved digital holography comprises (a) providing tunable timescale at least two tick-tock pulses arriving on a sample to be measured at various timescales, (b) spatial encoding for wavefronts of the sample by utilizing at least one optical encoder, (c) utilizing a digital holographic access unit to record a composite hologram of the spatial encoded wavefronts by the same single pulse source, (d) utilizing an encoder for obtaining at least two holograms, and (e) utilizing a compressive sensing approach or a non-linear image restoration approach and a wavefront reconstruction approach to obtain the reconstructed wavefronts information of the sample.
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What is claimed is: 1 . A method for time-resolved digital holography, comprising: providing at least two beam pulses with adjustable elapsed time which arrive on a sample under measuring at different time points; spatially encoding for a wavefront of said at least two beam pulses by an optical encoder; recording a one-shot composite digital hologram of said encoded spatial wavefront by a digital holographic access unit in a single exposing process; decoding said composite digital hologram to obtain at least two different digital holograms by at least one decoder; utilizing an image processing approach for processing said at least two different digital holograms to recover hologram data of said sample; and utilizing a wavefront reconstructing approach to reconstruct said hologram data to obtain a reconstructed wavefront of said sample. 2 . The method of claim 1 , wherein said optical encoder includes a spatial light modulator. 3 . The method of claim 1 , wherein said optical encoder includes a micro display. 4 . The method of claim 1 , wherein said digital holographic access unit includes a photodetector array. 5 . The method of claim 1 , wherein said digital holographic access unit includes an image sensor. 6 . The method of claim 1 , wherein said wavefront reconstructing approach includes Fourier transform approach. 7 . The method of claim 1 , wherein said wavefront reconstructing approach includes convolution approach. 8 . The method of claim 1 , wherein said wavefront reconstructing approach includes angular spectrum approach. 9 . The method of claim 1 , wherein said wavefront reconstructing approach includes Fresnel diffraction approximation approach. 10 . The method of claim 1 , wherein said image processing approach includes a compressive sensing approach. 11 . The method of claim 1 , wherein said image processing approach includes a non-linear image recovery approach. 12 . An apparatus for time-resolved digital holography, comprising: a pulsed laser for creating at least two beam pulses which arrive on a sample under measuring at different time points; an optical encoder for spatially encoding a wavefront of said at least two beam pulses; a digital holographic access unit for recording said encoded spatial wavefront of said at least two beam pulses; and a wavefront reconstruction unit to reconstruct said wavefront of said at least two beam pulses. 13 . The apparatus of claim 12 , wherein said optical encoder includes a spatial light modulator. 14 . The apparatus of claim 12 , wherein said optical encoder includes a micro display. 15 . The apparatus of claim 12 , wherein said digital holographic access unit includes a photodetector array. 16 . The apparatus of claim 12 , wherein said digital holographic access unit includes an image sensor. 17 . The apparatus of claim 12 , wherein said wavefront reconstruction unit includes at least one computer, used for decoding, separating wavefront of said at least two beam pulses. 18 . The apparatus of claim 12 , further comprising a beam splitting element configured in front of said pulsed laser. 19 . The apparatus of claim 12 , wherein said pulsed laser includes a Ti-sapphire laser. 20 . The apparatus of claim 12 , further comprising at least one fiber grating to split said at least two beam pulses.
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