Method for producing heavy chain aminocarboxylic acid

Patent metadata

Field	Value
Publication number	US-2018327724-A1
Application number	US-201615771812-A
Country	US
Kind code	A1
Filing date	Oct 27, 2016
Priority date	Oct 27, 2015
Publication date	Nov 15, 2018
Grant date	—

Abstract

The present invention relates to a method for producing a medium chain aminocarboxylic acid and, more particularly, to a recombinant microorganism in which a fatty aldehyde dehydrogenase gene in ω-oxidative metabolic pathway and a β-oxidative metabolic pathway-related gene are deleted and a ω-transaminase gene is introduced, and a method for producing a medium chain aminocarboxylic acid by culturing the recombinant microorganism. The recombinant microorganism of the present invention can prevent additional oxidation of fatty aldehyde and β-oxidative metabolism and also produce a medium chain aminocarboxylic acid, such as 12-aminodecane, as a raw material of nylon 12 from a substrate such as fatty acid with a high yield by introducing an amine group into a terminal thereof.

First claim

1 . A recombinant microorganism from which a fatty aldehyde dehydrogenase gene in an ω-oxidative metabolism pathway and β-oxidative metabolism pathway-related genes are deleted and into which an ω-transaminase gene is also introduced.

2 . The recombinant microorganism of claim 1 , wherein the fatty aldehyde dehydrogenase gene and the β-oxidative metabolism pathway-related genes are deleted from all homologous genes present in the microorganism.

3 . The recombinant microorganism of claim 1 , wherein the fatty aldehyde dehydrogenase gene and the β-oxidative metabolism pathway-related genes are deleted from some of the homologous genes present in the corresponding microorganism.

4 . The recombinant microorganism of claim 1 , wherein the fatty aldehyde dehydrogenase gene is selected from the group consisting of FALDH1, FALDH2, FALDH3, and FALDH4 genes.

5 . The recombinant microorganism of claim 4 , wherein each of the FALDH1, FALDH2, FALDH3, and FALDH4 genes comprise base sequences set forth in SEQ ID NOs: 1 to 4.

6 . The recombinant microorganism of claim 1 , wherein the β-oxidative metabolism pathway-related gene is an acyl-CoA oxidase gene.

7 . The recombinant microorganism of claim 6 , wherein the acyl-CoA oxidase gene is selected from the group consisting of ACO1, ACO2, ACO3, ACO4, ACO5, and ACO6 genes.

8 . The recombinant microorganism of claim 7 , wherein each of the ACO1, ACO2, ACO3, ACO4, ACO5, and ACO6 genes comprise base sequences set forth in SEQ ID NOs: 5 to 10, respectively.

9 . The recombinant microorganism of claim 1 , wherein the ω-transaminase gene comprises a base sequence set forth in SEQ ID NO: 11.

10 . The recombinant microorganism of claim 1 , wherein the microorganism is a yeast or Escherichia coli.

11 . The recombinant microorganism of claim 10 , wherein the yeast is selected from the group of the yeast consisting of Yarrowia sp., Saccharomyces sp., Pichia sp., and Candida sp.

12 . The recombinant microorganism of claim 11 wherein the yeast of Yarrowia sp. is Yarrowia lipolytica.

13 . A method for producing a medium chain aminocarboxylic acid, comprising:
(1) preparing the recombinant microorganism according to claim 1 ; and (2) treating the recombinant microorganism with a substrate to culture the recombinant microorganism.

14 . The method of claim 13 , wherein the substrate is a fatty acid.

15 . The method of claim 14 , wherein the fatty acid is a fatty acid having 5 to 30 carbon atoms.

16 . The method of claim 15 , wherein the fatty acid is dodecanoic acid.

17 . The method of claim 13 , wherein the medium chain aminocarboxylic acid is a medium chain aminocarboxylic acid compound having 5 to 30 carbon atoms.

18 . The method of claim 17 , wherein the medium chain aminocarboxylic acid is 12-aminododecanoic acid.

Assignees

• Korea Res Inst Bioscience & Biotechnology

Inventors

• Ahn Jung Oh
• Lee Hong Weon
• Park Gyu Yeon
• Jang Min Jeong
• Jeon Woo Young

Classifications

• C12P13/001

  Amines; Imines · CPC title

• C12Y102/01003

  Aldehyde dehydrogenase (NAD+) (1.2.1.3) · CPC title

• C12N9/001

  acting on the CH-CH group of donors (1.3) · CPC title

• C12N9/1096 Primary

  transferring nitrogenous groups (2.6) · CPC title

• C12Y206/01

  Transaminases (2.6.1) · CPC title