Chromatographic materials

Patent metadata

Field	Value
Publication number	US-2018326399-A1
Application number	US-201815911630-A
Country	US
Kind code	A1
Filing date	Mar 5, 2018
Priority date	May 15, 2012
Publication date	Nov 15, 2018
Grant date	—

Abstract

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

First claim

1 . A chromatographic stationary phase material for normal phase chromatography, high-pressure liquid chromatography, solvated gas chromatography, supercritical fluid chromatography, sub-critical fluid chromatography, carbon dioxide based chromatography, hydrophilic interaction liquid chromatography or hydrophobic interaction liquid chromatography represented by Formula I:
[X](W) a (Q) b (T) c   Formula I
wherein: X is a chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material, or a group of block copolymers thereof; W is an hydroxyl group on the surface of X; Q is represented by:

T is represented by one of:

wherein each Z group independently comprises:
a) a surface attachment group having the formula (B 1 ) x (R 5 ) y (R 6 ) z Si— wherein x is an integer from 1-3,
y is an integer from 0-2,
z is an integer from 0-2,
and x+y+z=3
each occurrence of R 5 and R 6 independently represents methyl, ethyl, n-butyl, iso-butyl, tert-butyl, iso-propyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, or a zwitterion group; and
B 1 represents a siloxane bond;

b) an attachment to a surface organofunctional hybrid group selected from the group consisting of a direct carbon-carbon bond, a heteroatom linkage, ester linkage, ether linkage, thioether linkage, amine linkage, amide linkage, imide linkage, urea linkage, carbonate linkage, carbamate linkage, heterocycle linkage, triazole linkage and urethane linkage; or
c) an adsorbed, surface group that is not covalently attached to the surface of X; and b and c are positive numbers, 0.05≤(b/c)≤100, and a≥0.

2 - 62 . (canceled)

63 . The chromatographic stationary phase material of claim 1 , wherein T is represented by:

64 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a plurality of particles.

65 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a monolith.

66 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material is in the form of a superficially porous material.

67 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has chromatographically enhancing pore geometry.

68 . The chromatographic stationary phase material of claim 64 , wherein the plurality of particles have sizes between about 1.5 and 5 microns.

69 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a surface area of about 25 to 1100 m 2 /g.

70 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a pore volume of about 0.2 to 2.0 cm 3 /g.

71 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has a micropore surface area of less than about 105 m 2 /g.

72 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic stationary phase material has an average pore diameter of about 20 to 1500 Å.

73 . The chromatographic stationary phase material of claim 1 , wherein Q has a combined surface coverage of ≥0.5 μmol/m 2 .

74 . The chromatographic stationary phase material of claim 1 , wherein Q has a combined surface coverage of ≥1.5 μmol/m 2 .

75 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of an inorganic material with a hybrid surrounding layer.

76 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of a hybrid material with an inorganic surface layer.

77 . The chromatographic stationary phase material of claim 1 , wherein the chromatographic core material consists essentially of a hybrid material with a different hybrid surface layer.

Assignees

• Waters Technologies Corp

Inventors

• Wyndham Kevin D
• Morris Michael F
• Brousmiche Darryl W
• Hill Jason F
• Fairchild Jacob N

Classifications

• B01D53/025

  with wetted adsorbents; Chromatography (analytical chromatography G01N30/00 - G01N30/96; for liquids B01D15/08) · CPC title

• B01D15/40

  using supercritical fluid as mobile phase or eluent · CPC title

• B01J20/3085

  Chemical treatments not covered by groups B01J20/3007 - B01J20/3078 · CPC title

• B01J20/285

  based on polymers · CPC title

• B01J20/28076

  being more than 1.0 ml/g · CPC title