Method of making nanocomposite from date palm tree and saudi bentonite
US-2024261760-A1 · Aug 8, 2024 · US
Method and Chromatography Medium
US2018304231A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2018304231-A1 |
| Application number | US-201615769400-A |
| Country | US |
| Kind code | A1 |
| Filing date | Oct 28, 2016 |
| Priority date | Nov 6, 2015 |
| Publication date | Oct 25, 2018 |
| Grant date | — |
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- Title
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- Abstract
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Abstract
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The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.
First claim
Opening claim text (preview).
1 . A method to produce a chromatography medium, the chromatography medium comprising porous beads coupled with dextran inside the pores and on the bead surface, the method comprising subjecting the chromatography medium to dextranase treatment. 2 . The method according to claim 1 , wherein the dextranase is coupled to a support particle. 3 . The method according to claim 2 , wherein the support molecule is larger than the pores of the chromatography media to be treated. 4 . The method according to claim 2 , wherein the support molecule comprises natural or synthetic chromatography beads or PEG (poly ethylene glycol). 5 . The method according to claim 2 , wherein the support particle is magnetic. 6 . The method according to claim 4 , wherein the dextranase-coupled support particle may enter the pores of the chromatography medium. 7 . The method according to claim 1 , wherein the chromatography medium is made of natural or synthetic resins. 8 . The method according to claim 7 , wherein the chromatography medium is made of agarose. 9 . The method according to claim 8 , wherein the agarose is cross-linked. 10 . The method according to claim 1 , wherein the dextran coupled to the chromatography medium is provided with a ligand, such as an ion exchange, affinity, hydrophobic interaction or multimodal ligand. 11 . A chromatography medium produced according to the method of claim 1 . 12 . A chromatography media according to claim 11 , wherein the beads are made of cross-linked agarose and the dextran is not provided with any ligands.
Assignees
Inventors
Classifications
Addition of material, later completely removed, e.g. as result of heat treatment, leaching or washing, e.g. for forming pores · CPC title
by ion-exchange (ion-exchange in general B01J) · CPC title
Chiral phases · CPC title
- B01J20/24Primary
Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives · CPC title
Particle form · CPC title
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Frequently asked questions
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- What does patent US2018304231A1 cover?
- The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.
- Who is the assignee on this patent?
- Ge Healthcare Bioprocess R&D Ab, Ge Healthcare Bio Sciences Ab
- What technology area does this patent fall under?
- Primary CPC classification B01J20/24. Mapped technology areas include Operations & Transport.
- When was this patent published?
- Publication date Thu Oct 25 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).