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Method of producing 2'-fucosyllactose using corynebacterium glutamicum
US2018298389A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2018298389-A1 |
| Application number | US-201715574028-A |
| Country | US |
| Kind code | A1 |
| Filing date | Apr 24, 2017 |
| Priority date | Apr 25, 2016 |
| Publication date | Oct 18, 2018 |
| Grant date | — |
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Abstract
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Disclosed are a recombinant Corynebacterium glutamicum (C. glutamicum) for producing fucosyllactose which is transformed to express α-1,2-fucosyltransferase, GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY), wherein the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and a method for producing fucosyllactose using the same. According to the recombinant Corynebacterium glutamicum and the method for producing fucosyllactose according to the present invention, with use of a GRAS Corynebacterium glutamicum strain, which is safer than conventional Escherichia coli, 2′-fucosyllactose can be produced at a high concentration while overcoming drawbacks of conventional methods associated with industrial inapplicability resulting from low production concentrations.
First claim
Opening claim text (preview).
1 . A recombinant Corynebacterium glutamicum transformed to express α-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase. 2 . The recombinant Corynebacterium glutamicum according to claim 1 , wherein the α-1,2-fucosyltransferase is encoded by fucT2 genes. 3 . The recombinant Corynebacterium glutamicum according to claim 2 , wherein the fucT2 genes have the nucleic acid sequence of SEQ ID NO: 6. 4 . The recombinant Corynebacterium glutamicum according to claim 1 , wherein the recombinant Corynebacterium glutamicum is transformed to overexpress phosphomannomutase, and transformed to overexpress GTP-mannose-1-phosphate guanylyltransferase. 5 . A method of producing 2′-fucosyllactose comprising culturing the recombinant Corynebacterium glutamicum according to claim 1 in a medium supplemented with lactose. 6 . The method according to claim 5 , wherein the medium further comprises glucose. 7 . The method according to claim 6 , wherein the production of the fucosyllactose is carried out by fed-batch culture comprising further supplying glucose or lactose.
Assignees
Inventors
Classifications
GDP-4-dehydro-D-rhamnose reductase (1.1.1.187) · CPC title
Galactoside 2-alpha-L-fucosyltransferase (2.4.1.69) · CPC title
3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase · CPC title
- C12N15/52Primary
Genes encoding for enzymes or proenzymes · CPC title
GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD · CPC title
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- What does patent US2018298389A1 cover?
- Disclosed are a recombinant Corynebacterium glutamicum (C. glutamicum) for producing fucosyllactose which is transformed to express α-1,2-fucosyltransferase, GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY), wherein the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and a method for producing fucosy…
- Who is the assignee on this patent?
- Seoul Nat Univ R&Db Foundation, Ap Tech Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N15/52. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Oct 18 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).