Candida carbonyl reductase and method for preparing (r)-lipoic acid precursor

Patent metadata

Field	Value
Publication number	US-2018265875-A1
Application number	US-201515307455-A
Country	US
Kind code	A1
Filing date	Mar 4, 2015
Priority date	Mar 4, 2015
Publication date	Sep 20, 2018
Grant date	—

Abstract

Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-α-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-α-α-lipoic acid.

First claim

1 . A Candida parapsilosis with the deposit number CGMCC 9630, wherein the Candida parapsilosis is able to express a carbonyl reductase.

2 . A carbonyl reductase, wherein the amino acid sequence of the carbonyl reductase is as set forth in SEQ ID Nos. 2, 4 or 6.

3 . A carbonyl reductase encoding gene, wherein the gene is:
(1) a nucleotide sequence as set forth in SEQ ID Nos. 1, 3 or 5; (2) an encoding gene of a protein with an amino acid sequence as set forth in SEQ ID Nos. 2, 4 or 6.

4 . A recombinant expression vector, wherein the vector comprises the gene according to claim 3 , and optionally an encoding gene of a glucose dehydrogenase, wherein the glucose dehydrogenase is as set forth in SEQ ID No. 14.

5 . A recombinant expression transformant, wherein the transformant comprises the recombinant expression vector according to claim 4 .

6 . A method of preparing a carbonyl reductase, wherein the method comprises a step of culturing the Candida parapsilosis according to claim 1 .

7 . The method of claim 6 , further comprising catalyzing an asymmetric reduction reaction of prochiral carbonyl compounds using the carbonyl reductase as a catalyst in the reduction reaction.

8 . The method according to claim 7 , wherein the concentration of the prochiral carbonyl compounds is 1 to 1500 mmol/L, and the amount of the carbonyl reductase is optionally 8.0˜800 U/mmol prochiral carbonyl compounds.

9 . The method according to claim 7 , wherein in the asymmetric reduction reaction, (1) NADPH or (2) glucose dehydrogenase, glucose and NADP + are further added, and
the amount of addition for coenzyme NADPH is 0 to 10 mmol/L, if added,
the amount of addition for glucose dehydrogenase is 0 to 340 U/mmol prochiral carbonyl compounds, if added,
the amount of addition for glucose is 0 to 0.3 g/mmol prochiral carbonyl compounds, if added, and
the amount of addition for coenzyme NADP + is 0 to 1.0 mmol/L, if added.

10 . The method according to claim 7 , wherein the prochiral carbonyl compounds are selected from the followings:

wherein R 1 is selected from —CH 2 (CH 3 ) 2 , -2-Cl—C 6 H 4 and —(CH 2 ) 2 C 6 H 5 ; R 2 is —CH 3 or —CH 2 CH 3 ; R 3 is —CH 2 Cl, —CH 2 CH 3 or —(CH 2 ) 2 CH 3 ; R 4 is —CH 3 or —CH 2 CH 3 .

11 . The method according to claim 10 , wherein the prochiral carbonyl compound is ethyl 6-carbonyl-8-chlorocaprylate.

Assignees

• Univ East China Science & Tech

Inventors

• Xu Jianhe
• Zhang Yujun
• Zheng Gaowei
• Pan Jiang

Classifications

• C12N1/16

  Yeasts; Culture media therefor · CPC title

• C12P7/42

  Hydroxy-carboxylic acids · CPC title

• C12Y101/01184

  Carbonyl reductase (NADPH) (1.1.1.184) · CPC title

• C12R1/72

  Chemistry & Metallurgy · mapped topic

• C12N15/81

  for yeasts · CPC title