Ceramic-containing bioactive inks and printing methods for tissue engineering applications

Patent metadata

Field	Value
Publication number	US-2018243484-A1
Application number	US-201815963348-A
Country	US
Kind code	A1
Filing date	Apr 26, 2018
Priority date	Aug 2, 2013
Publication date	Aug 30, 2018
Grant date	—

Abstract

Ink formulations comprising bioactive particles, methods of printing the inks into three-dimensional (3D) structures, and methods of making the inks are provided. Also provided are objects, such as tissue growth scaffolds and artificial bone, made from the inks, methods of forming the objects using 3D printing techniques, and method for growing tissue on the tissue growth scaffolds. The inks comprise a plurality of bioactive ceramic particles, a biocompatible polymer binder, optionally at least one bioactive factor, and a solvent.

First claim

What is claimed is:

1 . A porous tissue growth material comprising at least one layer comprising one or more porous fibers, wherein the one or more porous fibers comprise bioactive ceramic particles in a biocompatible polymer binder and the bioactive ceramic particles make up at least 70 weight percent of the one or more porous fibers, the tissue growth material being characterized in that it is osteogenically active and can undergo a reversible deformation when a compressive or tensile stress is applied and then removed.

2 . The material of claim 1 comprising a plurality of stacked layers, each layer in the plurality of stacked layers comprising one or more of the porous fibers.

3 . The materials of claim 1 comprising a plurality of the porous fibers.

4 . The material of claim 1 , wherein the bioactive ceramic particles make up at least 90 weight percent of the porous fibers.

5 . The material of claim 1 , wherein the bioactive ceramic particles are calcium phosphate particles.

6 . The material of claim 5 , wherein the biocompatible polymer binder comprises polylactic-co-glycolic acid or polylactide-co-glycolide.

7 . The material of claim 5 , wherein the biocompatible polymer binder comprises polycaprolactone.

8 . The material of claim 3 , wherein the porous fibers in the material are spaced apart and run substantially parallel with one another.

9 . The material of claim 1 , wherein the at least some portions of the one or more porous fibers run substantially parallel with one another and are in contact along their long axes.

10 . The material of claim 1 , wherein the one or more porous fibers include intra-fiber pores with diameters in the range from about 1 μm to about 10 μm.

11 . The material of claim 1 , wherein the one or more porous fibers have diameters greater than 100 μm.

12 . The material of claim 11 , wherein the one or more porous fibers have diameters of less than 400 μm.

13 . The material of claim 3 , wherein the porous fibers are spaced apart and intra-scaffold pores are defined by spaces between the fibers, the intra-scaffold pores having diameters of greater than 200 μm.

14 . The material of claim 1 further comprising living cells seeded into the material.

15 . The material of claim 14 , wherein the living cells comprise mesenchymal stem cells.

16 . The material of claim 1 , characterized in that it can undergo a deformation from an original shape to a deformed shaped under a compressive stress.

17 . A method of growing cells, tissue, or both using the material of claim 1 , the method comprising seeding the material with living cells, and culturing the cell-seeded material in a cell or tissue growth culture medium.

18 . A method of promoting in vivo tissue growth on the material of claim 1 , the method comprising implanting the material into a living animal.

19 . The method of claim 18 , wherein the animal is a human.

20 . The method of claim 18 , wherein the material comprises a plurality of stacked layers, each layer in the plurality of stacked layers comprising one or more of the porous fibers.

Assignees

• Univ Northwestern

Inventors

• Shah Ramille N
• Jakus Adam E

Classifications

• A61L2430/02

  for reconstruction of bones; weight-bearing implants · CPC title

• A61L27/56 Primary

  Porous materials, {e.g. foams or sponges} · CPC title

• C09D11/037

  characterised by the pigment · CPC title

• C09D11/322

  Pigment inks · CPC title

• A61L2430/06

  for cartilage reconstruction, e.g. meniscus · CPC title