Separation/purification apparatus

Patent metadata

Field	Value
Publication number	US-2018231510-A1
Application number	US-201515751500-A
Country	US
Kind code	A1
Filing date	Aug 24, 2015
Priority date	Aug 24, 2015
Publication date	Aug 16, 2018
Grant date	—

Abstract

A preparative purification apparatus capable of adjusting a mixing ratio of a mobile phase and an elution solvent contained in a solution to be collected. A liquid feeding unit which feeds an elution solvent to an inlet of a trap column; a flow path switching unit which selectively connects an outlet of the trap column to one of a waste liquid flow path or a recovery flow path; a liquid feed amount measurement unit; and a flow path control unit which performs a control such that the flow path switching unit is connected to the recovery flow path when the amount of the elution solvent fed to the trap column reaches a predetermined initial waste liquid amount, and thereafter, the flow path switching unit is connected to the waste liquid flow path at a timing of reaching a predetermined solution recovery amount.

First claim

1 - 4 . (canceled)

5 . A preparative purification apparatus which causes a solution eluted from a liquid chromatograph to flow through a trap column to capture a target component contained in the solution in the trap column, and thereafter, causes an elution solvent different from a mobile phase of the liquid chromatograph to flow through the trap column to elute the target component from the trap column, the apparatus comprising:
a) a liquid feeding unit which feeds the elution solvent to an inlet of the trap column; b) a flow path switching unit which selectively connects an outlet of the trap column to one of a waste liquid flow path and a recovery flow path; c) a liquid feed amount measurement unit which measures an amount of the elution solvent fed to the trap column by the liquid feeding unit; and d) a flow path control unit which controls the flow path switching unit to connect the outlet of the trap column to the recovery flow path at a timing when the amount of the elution solvent fed to the trap column, which is measured by the liquid feed amount measurement unit and, reaches a predetermined initial waste liquid amount, and thereafter, to connect the outlet of the trap column to the waste liquid flow path at a timing -when the amount of the elution solvent fed to the trap column, which is measured by the liquid feed amount measurement unit, reaches a predetermined solution recovery amount, wherein the mobile phase and the elution solvent in a mixing ratio determined by the predetermined initial waste liquid amount and the predetermined solution recovery amount are recovered from the recovery flow path without performing an evaporation and dryness process.

6 . The preparative purification apparatus according to claim 5 , wherein the elution solvent has a boiling point lower than that of water.

7 . The preparative purification apparatus according to claim 5 further comprising a plurality of the trap columns,
wherein, when the target components captured in the respective trap columns are sequentially eluted and recovered into individual recovery containers, the flow path control unit makes the mixing ratio of the mobile phase and the elution solvent recovered for each type of target component captured in each trap column constant, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values for the types of the target components captured in the respective trap columns

8 . The preparative purification apparatus according to claim 6 further comprising a plurality of the trap columns,
wherein, when the target components captured in the respective trap columns are sequentially eluted and recovered into individual collection containers, the flow path control unit makes the mixing ratio of the mobile phase and the elution solvent recovered for each type of target component captured in each trap column constant, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values for the types of the target components captured in the respective trap columns

9 . The preparative purification apparatus according to claim 5 , wherein, when the target component is recovered using the same sample, the flow path control unit constantly sets the mixing ratio of the recovered mobile phase and the elution solvent, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values.

10 . The preparative purification apparatus according to claim 6 , wherein, when the target component is recovered using the same sample, the flow path control unit constantly sets the mixing ratio of the recovered mobile phase and the elution solvent, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values.

11 . The preparative purification apparatus according to claim 7 , wherein, when the target component is recovered using the same sample, the flow path control unit constantly sets the mixing ratio of the recovered mobile phase and the elution solvent, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values.

12 . A preparative purification method in which a solution eluted from a liquid chromatograph is caused to flow through a trap column to capture a target component contained in the solution in the trap column, and thereafter, an elution solvent different from a mobile phase of the liquid chromatograph is caused to flow through the trap column to elute the target component from the trap column, the method comprising:
a) feeding the elution solvent to an inlet of the trap column; b) measuring an amount of the elution solvent fed to the trap column; and c) connecting the outlet of the trap column to the recovery flow path at a timing when the measured amount of the elution solvent fed to the trap column reaches a predetermined initial waste liquid amount, and thereafter, connecting the outlet of the trap column to the waste liquid flow path at a timing when the measured amount of the elution solvent fed to the trap column reaches a predetermined solution recovery amount, thereby recovering the mobile phase and the elution solvent in a mixing ratio determined by the predetermined initial waste liquid amount and the predetermined solution recovery amount from the recovery flow path without performing an evaporation and dryness process.

13 . The preparative purification method according to claim 12 , wherein, when the target components captured in the plurality of trap columns are sequentially eluted and recovered into individual collection containers, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values for types of the target components captured in the respective trap columns, the mixing ratio of the mobile phase and the elution solvent recovered for each type of target component captured in each trap column is made constant.

14 . The preparative purification method according to claim 12 , wherein, when the target component is recovered using the same sample, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values, the mixing ratio of the recovered mobile phase and the elution solvent is made constant.

15 . The preparative purification method according to claim 13 , wherein, when the target component is recovered using the same sample, by setting the predetermined initial waste liquid amount and the predetermined solution recovery amount to the same values, the mixing ratio of the recovered mobile phase and the elution solvent is made constant.

Assignees

• Shimadzu Corp

Inventors

• Yamazaki Tomoyuki
• Iwata Yosuke

Classifications

• G01N2030/027

  Liquid chromatography · CPC title

• G01N30/46

  using more than one column {(G01N30/44 takes precedence)} · CPC title

• G01N30/84

  Preparation of the fraction to be distributed · CPC title

• G01N30/466

  with separation columns in parallel · CPC title

• G01N30/80

  Fraction collectors · CPC title