Multi-Layered Biomimetic Material and Method of Formation

Patent metadata

Field	Value
Publication number	US-2018228938-A1
Application number	US-201615758528-A
Country	US
Kind code	A1
Filing date	Sep 8, 2016
Priority date	Sep 8, 2015
Publication date	Aug 16, 2018
Grant date	—

Abstract

Biocompatible biomimetic materials that exhibit desirable mechanical properties while also encouraging cell ingrowth and proliferation are described. The biomaterials include a multi-layer laminate of three or more decellularized aligned collagen tissues. The individual layers are aligned with one another in an angle-ply arrangement, with the collagen of each layer aligned at an angle to the collagen of the adjacent layer. The biomaterials are useful as collagenous graft materials such as a patch for a hernia in an annulus fibrosus or grafting materials for repair of tendons, ligaments, cartilage and other musculoskeletal tissues.

First claim

What is claimed is:

1 . A biocompatible multi-laminate, angle-ply construct comprising:
a first layer including a first decellularized tissue, the first decellularized tissue including collagen generally aligned in a first direction; a second layer including a second decellularized tissue, the second decellularized tissue including collagen generally aligned in a second direction, the first direction and the second direction defining an angle therebetween; a third layer including a third decellularized tissue, the third decellularized tissue including collagen generally aligned in a third direction, the third direction being essentially the same as the first direction, the second layer being between the first layer and the third layer; wherein the first, second and third layers are attached to one another.

2 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein the angle is from about 25° to about 90°.

3 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein the angle is about 25° or less.

4 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein one or more of the first, second, or third decellularized tissue comprises one or more layers.

5 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein two or more of the first, second, and third decellularized tissues are derived from the same source tissue.

6 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein one or more of the first, second, and third decellularized tissues is decellularized pericardium.

7 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein one or more of the first, second, and third decellularized tissues are crosslinked.

8 . The biocompatible multi-laminate, angle-ply construct of claim 1 , further comprising a hydrogel between at least the first and second layer.

9 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein the biocompatible construct is an annulus fibrosus patch.

10 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein the biocompatible construct is a musculoskeletal graft.

11 . The biocompatible multi-laminate, angle-ply construct of claim 1 , wherein the biocompatible construct exhibits a biaxial burst pressure of about 2 megapascals or greater, an ultimate tensile strength of about 3 megapascals or greater, and/or an elastic modulus of from about 12 megapascals to about 24 megapascals.

12 . A method for forming a biocompatible construct, the method comprising:
locating a first layer adjacent to a second layer, the first layer including a first decellularized tissue, the first decellularized tissue including collagen generally aligned in a first direction, the second layer including a second decellularized tissue, the second decellularized tissue including collagen generally aligned in a second direction, the first layer and the second layer being located adjacent to one another such that an angle is defined between the first direction and the second direction; locating a third layer adjacent to the second layer such that the second layer is between the first layer and the third layer, the third layer including a third decellularized tissue, the third decellularized tissue including collagen generally aligned in a third direction, the third layer being located such that the third direction and the first direction are essentially the same; and attaching the first, second, and third layers to one another.

13 . The method of claim 12 , the angle being from about 25° to about 90°.

14 . The method of claim 12 , the angle being about 25° or less.

15 . The method of claim 12 , further comprising decellularizing the first, second, and third tissues.

16 . The method of claim 12 , further comprising crosslinking one or more of the first, second, and third decellularized tissues.

17 . The method of claim 12 , wherein two or more of the first, second, and third decellularized tissues are derived from the same source tissue.

18 . The method of claim 12 , wherein one or more of the first, second, and third decellularized tissues is decellularized pericardium.

19 . The method of claim 12 , further comprising locating a hydrogel or a hydrogel precursor between at least the first and second layers.

Assignees

• Univ Clemson

Inventors

• Mcguire Rachel
• Borem Ryan
• Mercuri Jeremy

Classifications

• A61L27/3654

  Cartilage, e.g. meniscus · CPC title

• A61F2002/4495

  having a fabric structure, e.g. made from wires or fibres · CPC title

• A61L2430/10

  for reconstruction of tendons or ligaments · CPC title

• A61F2/442

  Intervertebral or spinal discs, e.g. resilient (non-disc-shaped intervertebral inflatable pockets A61F2/441; non-disc-shaped intervertebral fusion implants A61F2/4455) · CPC title

• A61L27/52

  Hydrogels or hydrocolloids · CPC title