Methods and Compositions for Generating Bioactive Assemblies of Increased Complexity and Uses
US-2015374846-A1 · Dec 31, 2015 · US
Modular, Multifunctional Nanoparticle-Based Bioconjugate for Realtime Visualization of Cellular Membrane Potential
US2018217153A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2018217153-A1 |
| Application number | US-201815882259-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jan 29, 2018 |
| Priority date | Jan 30, 2017 |
| Publication date | Aug 2, 2018 |
| Grant date | — |
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Abstract
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A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C 60 fullerene. Emission correlates with cellular membrane potential.
First claim
Opening claim text (preview).
What is claimed is: 1 . A construct for detecting cellular membrane potential, comprising: a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. 2 . The construct of claim 1 , wherein the modular peptide is selected from the group consisting of SEQ ID No: 1, SEQ ID No: 2, and SEQ ID No: 3. 3 . The construct of claim 1 , wherein the nanoparticle is a quantum dot having a hydrophilic coating. 4 . The construct of claim 1 , wherein the electron acceptor is C 60 . 5 . A construct for detecting cellular membrane potential, comprising: a quantum dot operable as an electron donor and having a hydrophullilc coating; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor comprising C 60 . 6 . The construct of claim 5 , wherein the modular peptide is selected from the group consisting of SEQ ID No: 1, SEQ ID No: 2, and SEQ ID No: 3. 7 . A method of detecting cellular membrane potential, the method comprising: providing a construct comprising a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor; contacting a cell with the construct; and detecting emission from the construct, wherein the emission correlates with cellular membrane potential. 8 . The method of claim 7 , wherein the modular peptide is selected from the group consisting of SEQ ID No: 1, SEQ ID No: 2, and SEQ ID No: 3. 9 . The method of claim 7 , wherein the nanoparticle is a quantum dot. 10 . The method of claim 7 , wherein the electron acceptor is C 60 .
Assignees
Inventors
Classifications
Intracellular protein regulatory factors and their receptors, e.g. including ion channels · CPC title
Nanoparticles · CPC title
with steric inhibition or signal modification, e.g. fluorescent quenching · CPC title
containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins · CPC title
- G01N33/588Primary
with semiconductor nanocrystal label, e.g. quantum dots · CPC title
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- What does patent US2018217153A1 cover?
- A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nano…
- Who is the assignee on this patent?
- Us Gov Sec Navy
- What technology area does this patent fall under?
- Primary CPC classification G01N33/588. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Thu Aug 02 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).