Aqueous composition comprising at least one protein and one solubilizing agent, preparation thereof and uses thereof

Patent metadata

Field	Value
Publication number	US-2018214556-A1
Application number	US-201715694534-A
Country	US
Kind code	A1
Filing date	Sep 1, 2017
Priority date	May 14, 2014
Publication date	Aug 2, 2018
Grant date	—

Abstract

The present invention relates to a liquid composition which comprises, in an aqueous medium, one or more protein(s) and one or more solubilizing agent(s) chosen from the group consisting of anionic compounds of non-saccharide structure, said structure of which contains at least one aromatic nucleus comprising at least 6 ring members (6 atoms) and at least one carboxylic acid group in salified form, and which has, in its acid form, a molar mass of between 130 and 500 g/mol.
It also relates to the use of said solubilizing agent(s) for preparing compositions according to the invention.
It also relates to a process for solubilizing one or more protein(s), wherein at least one solubilizing agent chosen from the group consisting of anionic compounds of non-saccharide structure, said structure of which contains at least one aromatic nucleus comprising at least 6 ring members (6 atoms) and at least one carboxylic acid group in salified form, and which has, in its acid form, a molar mass of between 130 and 500 g/mol, is added to an aqueous protein preparation in order to solubilize the protein.

First claim

What is claimed is:

1 . A process for solubilizing one or more protein(s) in water, wherein at least one solubilizing agent chosen from the group consisting of anionic compounds of non-saccharide structure, said structure of which contains at least one aromatic nucleus comprising at least 6 ring members (6 atoms) and at least one carboxylic acid group in salified form and which, in its acid form, has a molar mass of between 130 and 500 g/mol, is added to an aqueous protein composition.

2 . A process for solubilizing proteins within an aqueous composition, of at least one solubilizing agent chosen from the group consisting of anionic compounds of non-saccharide structure, said structure of which contains at least one aromatic nucleus comprising at least 6 ring members (6 atoms) and at least one carboxylic acid group in salified form, and which has, in its acid form, a molar mass of between 130 and 500 g/mol.

3 . The process for solubilizing agent(s) correspond(s) to general formula (I) below:

with:
Ar denoting an aromatic nucleus comprising at least 6 ring members;
X denoting a saturated or unsaturated, linear or branched divalent radical, the main chain of which consists of 1 to 4 carbon atoms and optionally 1 or 2 heteroatoms chosen from nitrogen and oxygen atoms, it being possible for said main chain to optionally bear one or more substituents;
Y1 and Y2 denoting, independently of one another: a hydrogen atom; an —OH group; an —OR1 group with R1 denoting an alkyl radical containing from 1 to 6 carbon atoms or a hydroxyalkyl radical containing from 1 to 6 carbon atoms; and preferably Y1 and Y2 denote, independently of one another, a hydrogen atom or an —OH group; and
M denoting a cation.

4 . The process as claimed in claim 3 , wherein, in formula (I), Ar denotes a benzene nucleus or an indole nucleus.

5 . The process as claimed in claim 3 , wherein the solubilizing agent(s) correspond(s) to formula (Ia) below:

with X, Y1, Y2 and M as defined in claim 3 .

6 . The process as claimed in claim 3 , wherein the solubilizing agent(s) correspond(s) to formula (Ib) below:

with X, Y1, Y2 and M as defined in claim 3 .

7 . The process as claimed in claims 3 , wherein, in formula (I), (Ia) or (Ib), the divalent radical X bears, on its main chain, one or more substituents corresponding to the general formula:
-L-Z with: L denotes a single bond or a group chosen from an amide group —NHCO—, a carbamate group —NHCOO— or a urea group —NHCONH—; and Z denotes a hydroxyl group (—OH); a saturated or unsaturated, linear or branched radical comprising from 1 to 4 carbon atoms, said carbon atoms bearing at least one hydroxyl group —[CH2]X-[OH]Y; a salified carboxylic acid group (—COOM′ with M′=Na+or K+); or a saturated or unsaturated, linear or branched radical comprising from 1 to 12 carbon atoms and optionally one or more heteroatoms.

8 . The process as claimed in claim 7 , wherein L denotes a single bond or an amide group.

9 . The process as claimed in claim 1 , wherein the solubilizing agent is resulting from a natural or synthetic amino acid bearing an aromatic ring, chosen from phenylalanine, tyrosine and tryptophan, and phenylalanine.

10 . The process as claimed in claim 1 , wherein the solubilizing agent is chosen from the group consisting of N-hydroxyacetylphenylalanine and N-hydroxyacetyltryptophan, corresponding to the formulae below:

used in the form of a sodium salt or of a potassium salt.

11 . The process as claimed in claim 1 , wherein the solubilizing agent is resulting from a phenol.

12 . The process as claimed in claim 1 , wherein the solubilizing agent is chosen from the following compounds, used in the form of sodium salts or of potassium salts:

Name
Structure

Phenylacetic acid

Mandelic acid

Hydrocinnamic acid

Trans-cinnamic acid

2-Phenoxypropionic acid

3-Phenyllactic acid

Phenylsuccinic acid

Alpha-hydroxyhippuric acid

13 . The process as claimed in claim 1 , wherein said aqueous composition comprises the solubilizing agent(s) in a total concentration of between 1 g/l and 100 g/l.

14 . The process as claimed in claim 1 , wherein said aqueous composition contains a total concentration of protein(s) of between 0.5 and 400 mg/ml.

15 . The process as cl

Assignees

• Adocia

Inventors

• Soula Rémi

Classifications

• A61P37/00

  Drugs for immunological or allergic disorders · CPC title

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61P3/00

  Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00) · CPC title

• A61K39/39516

  from serum, plasma · CPC title

• A61K47/183

  Amino acids, e.g. glycine, EDTA or aspartame · CPC title