Method for imaging biologic fluid samples using a predetermined distribution

Patent metadata

Field	Value
Publication number	US-2018156773-A1
Application number	US-201815886324-A
Country	US
Kind code	A1
Filing date	Feb 1, 2018
Priority date	Dec 6, 2012
Publication date	Jun 7, 2018
Grant date	—

Abstract

A method for analyzing a biologic fluid sample includes the steps of: a) providing a spatially mapped chamber; b) providing a predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample, which distribution indicates the presence or absence of a statistically significant number of constituents within the sample in each chamber sub-region; c) selecting one or more image techniques for each sub-region based on the presence or absence of the statistically significant number of one or more constituents in that sub-region as indicated by the distribution; d) creating image data representative of the biologic fluid sample in each sub-region, using the one or more image techniques selected for that sub-region; and e) analyzing the sample.

First claim

What is claimed is:

1 . A method for imaging a biologic fluid sample having one or more constituents, which sample is quiescently disposed within an analysis chamber, comprising:
spatially mapping a biologic fluid sample quiescently residing within an analysis chamber, which biologic fluid sample includes one or more constituents, the spatial mapping defining a plurality of sub-regions; providing a predetermined repeatable non-uniform spatial distribution representative of at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber, wherein the distribution indicates a presence or absence of the at least one constituent residing in select one or more sub-regions of the spatially mapped biologic fluid sample quiescently residing within the analysis chamber; selecting at least one imaging method for imaging the select one or more sub-regions based on the presence or absence of the at least one constituent in the select one or more sub-regions as indicated by the distribution; and creating image data representative of the biologic fluid sample in the select one or more sub-regions, using the at least one imaging method.

2 . The method of claim 1 , wherein the biologic fluid sample is blood and the constituents include at least one of white blood cells, red blood cells, plasma, or sphered red blood cells.

3 . The method of claim. 1 , wherein the chamber is a microscope slide.

4 . The method of claim 1 , wherein the step of creating image data representative of the biologic fluid sample in the select one or more sub-regions includes creating image data representative of the biologic fluid sample in all sub-regions.

5 . The method of claim 1 , wherein the predetermined repeatable non-uniform spatial distribution of at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber indicates a plurality of sub-regions where white blood cells are likely present.

6 . The method of claim 1 , wherein the predetermined repeatable non-uniform spatial distribution of at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber indicates a plurality of sub-regions where red blood cells are likely present.

7 . The method of claim 1 , further comprising analyzing the biologic fluid sample, using the image data representative of the biologic fluid sample in the select one or more sub-regions.

8 . The method of claim 1 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber in the select one or more sub-regions is based on empirical data.

9 . An apparatus for imaging a biologic fluid sample within a chamber configured to quiescently hold the biologic fluid sample, the apparatus comprising:
at least one sample illuminator; at least one image dissector; and a processor in communication with the at least one sample illuminator, the at least one image dissector, and a non-transitory memory storing instructions, which instructions include a predetermined repeatable non-uniform spatial distribution representative of at least one constituent within the biologic fluid sample quiescently disposed within the chamber, wherein the spatial distribution indicates a presence or an absence of the at least one constituent residing in particular sub-regions of the biologic fluid sample quiescently residing within the chamber, and when executed the instructions cause the processor to:
spatially map the biologic fluid sample quiescently residing within the analysis chamber, the spatial mapping defining the sub-regions of the biologic fluid sample quiescently residing within the chamber;
select at least one imaging method for imaging select one or more of the sub-regions based on the presence or absence of the at least one constituent in the select sub-regions as indicated by the distribution;
control the sample illuminator and the image dissector to create image data representative of the biologic fluid sample in the select one or more sub-regions, using the at least one imaging method.

10 . The apparatus of claim 9 , wherein the chamber is a microscope slide.

11 . The apparatus of claim 9 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently disposed within the chamber indicates a plurality of sub-regions where white blood cells are likely present.

12 . The apparatus of claim 9 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently disposed within the chamber indicates a plurality of sub-regions where red blood cells are likely present.

13 . The apparatus of claim 9 , wherein the biologic fluid sample is blood and the constituents include at least one of white blood cells, red blood cells, plasma, or sphered red blood cells.

14 . The apparatus of claim 9 , wherein the predetermined repeatable non-uniform spatial distribution representative of the at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber in the select one or more sub-regions is based on empirical data.

Assignees

• Abbott Point Of Care Inc

Inventors

• Xie Min
• Wardlaw Stephen C

Classifications

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G01N33/483

  Physical analysis of biological material · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N15/0227

  using imaging; using holography · CPC title

• G01N2015/0065

  Physics · mapped topic