Compositions and methods for urinary bladder regeneration

Patent metadata

Field	Value
Publication number	US-2018154045-A1
Application number	US-201715652983-A
Country	US
Kind code	A1
Filing date	Jul 18, 2017
Priority date	Jun 16, 2009
Publication date	Jun 7, 2018
Grant date	—

Abstract

The present invention provides compositions and methods for the regeneration of tissue. In particular, the present invention provides mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) for bladder regeneration.

First claim

1 - 19 . (canceled)

20 . A method for urinary bladder regeneration in a subject comprising transplanting mesenchymal stem cells and endothelial progenitor cells onto existing bladder tissue in the subject, wherein the mesenchymal stem cells are CD29+, CD44+, CD105+, and CD169+ and are at least 98% pure, and wherein the endothelial progenitor cells are CD34+ and CD133+ and are at least 98% pure.

21 . The method of claim 20 , wherein the existing bladder tissue is injured or diseased tissue.

22 . The method of claim 21 , wherein the existing bladder tissue is injured as the result of trauma.

23 . The method of claim 21 , wherein the subject has bladder cancer.

24 . The method of claim 21 , wherein the subject has spina bifida.

25 . The method of claim 20 , wherein the mesenchymal stem cells and endothelial progenitor cells are autologous to the subject.

26 . The method of claim 20 , wherein the mesenchymal stem cells and endothelial progenitor cells can be differentiated.

27 . The method of claim 26 , wherein the mesenchymal stem cells and endothelial progenitor cells differentiate into bladder tissue in the subject.

28 . The method of claim 20 , wherein the mesenchymal stem cells and endothelial progenitor cells scaffold, and the scaffold is transplanted onto the existing bladder tissue in the subject.

29 . The method of claim 28 , wherein the scaffold is a thin-film scaffold.

30 . The method of claim 29 , wherein the thin-film scaffold comprises poly(1,8 octanediol-co-citrate) (POC).

31 . The method of claim 20 , wherein the mesenchymal stem cells and endothelial progenitor cells comprise adult mesenchymal stem cells and endothelial progenitor cells.

32 . The method of claim 20 , wherein the mesenchymal stem cells and endothelial progenitor cells comprise pediatric mesenchymal stem cells and endothelial progenitor cells.

Assignees

• Univ Northwestern

Inventors

• Sharma Arun
• Hota Partha
• Fuller Natalie
• Matoka Derek
• Cheng Earl

Classifications

• A61K35/44

  Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells · CPC title

• A61L2430/22

  for reconstruction of hollow organs, e.g. bladder, esophagus, urether, uterus · CPC title

• A61L27/3886 Primary

  comprising two or more cell types · CPC title

• A61K35/28

  Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells · CPC title

• A61P13/10

  of the bladder · CPC title