Device and method for supervising biofilm culturing

Patent metadata

Field	Value
Publication number	US-2018136168-A1
Application number	US-201815871145-A
Country	US
Kind code	A1
Filing date	Jan 15, 2018
Priority date	Nov 9, 2016
Publication date	May 17, 2018
Grant date	—

Abstract

A device for supervising biofilm culturing, the device including: a fixed seat including an upper end; a supervision chamber; two ultrasonic sensing probes; a first mounting chamber; a second mounting chamber disposed between the fixed seat and the supervision chamber; an ultrasonic generator; an oscilloscope; and a controller. The first and second mounting chambers are disposed at two opposite ends of the supervision chamber, respectively. The two ultrasonic sensing probes are disposed in the first and second mounting chambers, respectively. The second mounting chamber is connected to the upper end of the fixed seat. The two ultrasonic sensing probes each are respectively connected to the ultrasonic generator, the oscilloscope, and the controller successively.

First claim

The invention claimed is:

1 . A device for supervising biofilm culturing, the device comprising:
a fixed seat comprising an upper end; a supervision chamber; two ultrasonic sensing probes; a first mounting chamber; a second mounting chamber disposed between the fixed seat and the supervision chamber; an ultrasonic generator; an oscilloscope; and a controller; wherein the first and second mounting chambers are disposed at two opposite ends of the supervision chamber, respectively; the two ultrasonic sensing probes are disposed in the first and second mounting chambers, respectively; the second mounting chamber is connected to the upper end of the fixed seat; and the two ultrasonic sensing probes each are respectively connected to the ultrasonic generator, the oscilloscope, and the controller successively.

2 . The device of claim 1 , wherein the fixed seat comprises a circular pedestal and a semi-open cylinder.

3 . The device of claim 1 , wherein the supervision chamber is transparent and comprises a side wall and a bottom wall; the side wall comprises a water inlet and a water outlet which is located higher than the water inlet; and the bottom wall comprises a groove for receiving a filler film.

4 . The device of claim 2 , wherein the supervision chamber is transparent and comprises a side wall and a bottom wall; the side wall comprises a water inlet and a water outlet which is located higher than the water inlet; and the bottom wall comprises a groove for receiving a filler film.

5 . The device of claim 3 , wherein the first mounting chamber is disposed on the supervision chamber; the first mounting chamber comprises a cylindrical holder and a chamber for accommodating one of the two ultrasonic sensing probes; the chamber for accommodating the one of the two ultrasonic sensing probes is disposed on an upper surface of the cylindrical holder; a first fixation device is disposed in the chamber to fix the one of the two ultrasonic sensing probes; the one of the two ultrasonic sensing probes is fixed on a side wall of the first fixation device; the cylindrical holder comprises a side wall and a plurality of slots disposed on the side wall; the slots correspond to the water inlet and the water outlet of the supervision chamber; the second mounting chamber comprises a second fixation device for accommodating the other of the two ultrasonic sensing probes; and the other of the two ultrasonic sensing probes is fixed on a side wall of the second fixation device.

6 . The device of claim 4 , wherein the first mounting chamber is disposed on the supervision chamber; the first mounting chamber comprises a cylindrical holder and a chamber for accommodating one of the two ultrasonic sensing probes; the chamber for accommodating the one of the two ultrasonic sensing probes is disposed on an upper surface of the cylindrical holder; a first fixation device is disposed in the chamber to fix the one of the two ultrasonic sensing probes; the one of the two ultrasonic sensing probes is fixed on a side wall of the first fixation device; the cylindrical holder comprises a side wall and a plurality of slots disposed on the side wall; the slots correspond to the water inlet and the water outlet of the supervision chamber 4 ; the second mounting chamber comprises a second fixation device for accommodating the other of the two ultrasonic sensing probes; and the other of the two ultrasonic sensing probes is fixed on a side wall of the second fixation device.

7 . The device of claim 5 , wherein the two ultrasonic sensing probes are coated with a coupling agent and then fixed on sides wall of the first and second fixation devices.

8 . The device of claim 6 , wherein the two ultrasonic sensing probes are coated with a coupling agent and then fixed on the sides wall of the first and second fixation devices.

9 . The device of claim 5 , wherein the cylindrical holder of the first mounting chamber comprises a bottom edge, and an annular groove and a gasket are disposed on the bottom edge; the first mounting chamber and the supervision chamber are fixed and sealed using the annular groove and the gasket.

10 . The device of claim 6 , wherein the cylindrical holder of the first mounting chamber comprises a bottom edge, and an annular groove and a gasket are disposed on the bottom edge; the first mounting chamber and the supervision chamber are fixed and sealed using the annular groove and the gasket.

11 . A method for supervising biofilm culturing, the method comprising:
1) sampling a mixture of target muddy water, and homogenizing the mixture; 2) filtering the homogenized mixture of muddy water, and collecting a filtrate resulting therefrom; 3) dividing the filtrate into two parts, labeling one of the two parts as Filtrate A; filtering the other part of the two parts using a filter membrane to yield another filtrate which is labeled as Filtrate B; 4) assembling the device for supervising biofilm culturing of claim; 5) fixing a first filler film in the supervision chamber; 6) pumping the Filtrate A into the supervision chamber via a water inlet thereof; 7) starting the ultrasonic generator when the Filtrate A is about to overflow from a water outlet of the supervision chamber, turning on an ultrasonic reflection mode, adjusting working parameters of the ultrasonic generator to preset values, opening the oscilloscope, and on-line supervising the biofilm culturing on the first filler film through the controller; 8) substituting the Filtrate B for the Filtrate A, and repeating 6)-7); 9) recording a time with regard to the biofilm culturing on the first filler film according to an oscillogram on the oscilloscope; 10) replacing the first filler film using a second filler film, repeating 5)-9), and recording a time with regard to the biofilm culturing on the second filler film; and 11) based on the observation results in 9-10), selecting a filler with which the biofilm culturing is the quickest to use in a target water body for sewage treatment.

12 . The method of claim 11 , wherein in 1), the mixture is homogenized for 10-15 times.

13 . The method of claim 11 , wherein in 2), the homogenized mixture of muddy water is filtered using an 8-10 μm filter membrane; in 3), the other part of the two parts is filtered using a 0.22-0.45 μm filter membrane.

14 . The method of claim 11 , wherein in 6), a flow rate of the Filtrate A into the supervision chamber is 0.5-10 mL/min.

15 . The method of claim 11 , wherein in 7), the working parameters of the ultrasonic generator 9 are as follows: repetition frequency, 15-25 Hz; pulse resistance, 30-60Ω; pulse voltage, 200-400 V; gain, 60 dB; high-pass filtering, 0.03-0.1 mHz; and low-pass filtering, 1-3 mHz.

Assignees

• Nanjing University Of Technology

Inventors

• Ren Hongqiang
• Wang Jinfeng
• Ding Lili
• Li Jianxin
• Fu Huimin
• Huang Hui

Classifications

• G01N29/348

  with frequency characteristics, e.g. single frequency signals, chirp signals (measuring frequency of mechanical vibrations or acoustic waves in general G01H1/06, G01H3/04; measuring frequency or analysing frequency spectra G01R23/00) · CPC title

• G01N29/223

  Supports, positioning or alignment in fixed situation (mounting transducers per se G10K11/004) · CPC title

• G01N29/036 Primary

  by measuring frequency or resonance of acoustic waves · CPC title

• C02F3/006

  Regulation methods for biological treatment · CPC title

• C02F3/12

  Activated sludge processes · CPC title