New polypeptide having a polyester degrading activity and uses thereof

Patent metadata

Field	Value
Publication number	US-2018051264-A1
Application number	US-201615556655-A
Country	US
Kind code	A1
Filing date	Mar 11, 2016
Priority date	Mar 13, 2015
Publication date	Feb 22, 2018
Grant date	—

Abstract

The present invention relates to a new isolated polypeptide comprising an amino acid sequence having at least 75%, 80%, 85%, 90%, 95%, 99% or 100% identity to the full length amino acid sequence set forth in SEQ ID NO:1, and having a polyester degrading activity, and uses thereof.

First claim

1 - 20 . (canceled)

21 . A polypeptide having a polyester degrading activity and having at least 90% identity to the full length amino acid sequence of SEQ ID NO: 1.

22 . The polypeptide of claim 21 , further comprising an amino acid sequence with at least 75% identity to the full length amino acid sequence of SEQ ID NO: 3.

23 . The polypeptide of claim 21 , which is active in a range of temperatures between 20° C. and 70° C.

24 . The polypeptide of claim 21 , which is active in a range of pH of 5-11.

25 . The polypeptide of claim 21 , which has a degrading activity selecting from a polylactic acid (PLA) degrading activity, a poly(L-lactic acid) (PLLA) degrading activity, a poly(D-lactic acid) (PDLA) degrading activity, a polybutylene succinate (PBS) degrading activity, and a polyhydroxyalkanoates (PHAs) degrading activity.

26 . A nucleic acid encoding the polypeptide of claim 21 .

27 . An expression cassette comprising the nucleic acid of claim 26 .

28 . A vector comprising the nucleic acid of claim 26 .

29 . A recombinant cell containing a nucleic acid of claim 26 .

30 . An isolated bacterial strain of the genus Micromonospora expressing a polypeptide of claim 21 .

31 . A method of producing a polypeptide, comprising:
(i) culturing a recombinant cell of claim 29 and (ii) recovering the culture supernatant or the cells.

32 . A method of producing a polypeptide, comprising:
(i) culturing a recombinant cell of or a Micromonospora strain of claim 30 and (ii) recovering the culture supernatant or the cells.

33 . A composition comprising a polypeptide of claim 21 .

34 . The composition of claim 33 , wherein said composition is selected from a liquid solution, a solid composition, and a lyophilized composition.

35 . A method for degrading a polyester containing material, comprising the step of exposing the polyester containing material to a polypeptide of claim 21 .

36 . The method of claim 35 , wherein at least one polyester of the polyester containing material is depolymerized up to monomers and/or oligomers.

37 . The method of claim 35 , comprising a pretreatment step of the polyester containing material to submit the polyester containing material to at least one modification selected from mechanical modification, physical modification, chemical modification, biological modification.

38 . The method of claim 35 , comprising a subsequent step of b) recovering the resulting monomers and/or oligomers.

39 . A plastic compound containing a polypeptide of claim 21 and at least one polyester.

40 . A process for producing the plastic compound, wherein at least one polyester and a polypeptide of claim 21 are mixed at a temperature at which the polyester is in a partially or totally molten state.

Assignees

• Carbios
• Centre Nat Rech Scient
• Univ Poitiers

Inventors

• Li Luen Luen
• Ferreira Thierry
• Aucher Willy
• Colas Steven
• Kadri Linette Lionelle

Classifications

• C12P7/56

  Lactic acid · CPC title

• C12P7/625

  Polyesters of hydroxy carboxylic acids · CPC title

• C12N15/00

  Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00) · CPC title

• C08J11/105

  by treatment with enzymes · CPC title

• C12P7/42

  Hydroxy-carboxylic acids · CPC title