Optical measurement device and optical measurement method

Patent metadata

Field	Value
Publication number	US-2018045571-A1
Application number	US-201515556190-A
Country	US
Kind code	A1
Filing date	Mar 11, 2015
Priority date	Mar 11, 2015
Publication date	Feb 15, 2018
Grant date	—

Abstract

In multiplex CARS, a fingerprint region is a region where signals are densely concentrated. Information about the intensities and spatial distribution of these signals is important in cell analysis. However, there has been a problem in that the signal intensities are low. Accordingly, mechanisms 702 and 703 for adjusting the power branching ratio between light that enters a photonic crystal fiber 705 and pumping light; mechanisms 710 and 711 for adjusting the divergence/convergence state of the pumping light; and mechanisms 706 and 2101 for adjusting the divergence/convergence state of Stokes light are provided to enable adjustment for emphasizing a desired wavelength band.

First claim

1 . An optical measurement device comprising:
a short pulse laser light source; a splitting unit configured to split emitting light from the short pulse laser light source into a first light flux and a second light flux; an optical fiber configured to generate supercontinuum light from the first light flux; a combining unit configured to combine a long wavelength component of the supercontinuum light serving as Stokes light with the second light flux serving as pumping light; a focus optical system configured to focus light combined in the combining unit onto a sample; a spectroscope configured to detect light generated from the sample; and a light amount ratio adjusting unit configured to adjust a light amount ratio between the first light flux and the second light flux in the splitting unit based on an intensity of a desired band of a spectrum detected in the spectroscope.

2 . The optical measurement device according to claim 1 , comprising:
a second optical fiber configured to cause the second light flux serving as the pumping light to pass.

3 . The optical measurement device according to claim 1 , comprising:
an adjusting unit configured to adjust a focal point of the Stokes light in the sample.

4 . The optical measurement device according to claim 1 , comprising:
an adjusting unit configured to adjust a focal point of the pumping light in the sample.

5 . The optical measurement device according to claim 4 , wherein the adjusting unit includes a spatial light phase modulator.

6 . An optical measurement device comprising:
a short pulse laser light source; a splitting unit configured to split emitting light from the short pulse laser light source into a first light flux and a second light flux; an optical fiber configured to generate supercontinuum light from the first light flux; a combining unit configured to combine a long wavelength component of the supercontinuum light serving as Stokes light with the second light flux serving as pumping light; a focus optical system configured to focus light combined in the combining unit onto a sample; a spectroscope configured to detect light generated from the sample; and an adjusting unit configured to adjust a focal point of the Stokes light in the sample based on an intensity of a desired band of a spectrum detected in the spectroscope.

7 . The optical measurement device according to claim 6 , wherein the adjusting unit adjusts a divergence/convergence amount of the Stokes light.

8 . The optical measurement device according to claim 6 , comprising:
a second optical fiber configured to cause the second light flux serving as the pumping light to pass.

9 . An optical measurement device comprising:
a short pulse laser light source; a splitting unit configured to split emitting light from the short pulse laser light source into a first light flux and a second light flux; an optical fiber configured to generate supercontinuum light from the first light flux; a combining unit configured to combine a long wavelength component of the supercontinuum light serving as Stokes light with the second light flux serving as pumping light; a focus optical system configured to focus light combined in the combining unit onto a sample; a spectroscope configured to detect light generated from the sample; and an adjusting unit configured to adjust a focal point of the pumping light in the sample based on an intensity of a desired band of a spectrum detected in the spectroscope.

10 . The optical measurement device according to claim 9 , wherein the adjusting unit adjusts a divergence/convergence amount of the pumping light.

11 . The optical measurement device according to claim 9 , comprising:
a second optical fiber configured to cause the second light flux serving as the pumping light to pass.

12 . The optical measurement device according to claim 9 , wherein the adjusting unit includes a spatial light phase modulator.

13 . An optical measurement method comprising:
splitting emitting light from a short pulse laser light source into a first light flux and a second light flux; coaxially combining a long wavelength component of supercontinuum light generated from the first light flux serving as Stokes light with the second light flux serving as pumping light; and detecting a spectrum generated by focusing combined light onto a sample, wherein, based on an intensity of a desired band of the detected spectrum, at least any of adjustments (1), (2), and (3) is performed: (1) an adjustment of a light amount ratio between the first light flux and the second light flux, (2) an adjustment of a focal point of the Stokes light in the sample, and (3) an adjustment of a focal point of the pumping light in the sample.

14 . The optical measurement device according to claim 6 , wherein the adjustment of the focus position of the Stokes light in the sample is performed by adjusting in a direction of an optical axis a position of a lens provided in an optical path for the Stokes light.

15 . The optical measurement device according to claim 9 , wherein the adjustment of the focus position of the pumping light in the sample is performed by adjusting in a direction of an optical axis a position of a lens provided in an optical path for the pumping light.

16 . The optical measurement device according to claim 6 , wherein the adjustment of the focus position of the Stokes light in the sample is performed at a plurality of wavenumber bands, and
wherein measurement results at the plurality of wavenumber bands are connected and displayed.

Assignees

• Hitachi High Tech Corp

Inventors

• Shiozawa Manabu
• Watanabe Koichi
• Shirai Masataka

Classifications

• G01J3/0237

  Adjustable, e.g. focussing · CPC title

• G01N21/65 Primary

  Raman scattering · CPC title

• G01J3/0218

  using optical fibers · CPC title

• G01J3/0208

  using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction · CPC title

• G01J3/4412 Primary

  Scattering spectrometry (particle sizing by light scattering G01N15/0205; optical velocimetry of particles G01P5/20, G01P5/26) · CPC title