Mass spectrometric determination of eicosapentaenoic acid and docosahexaenoic acid

Patent metadata

Field	Value
Publication number	US-2018024151-A1
Application number	US-201715635509-A
Country	US
Kind code	A1
Filing date	Jun 28, 2017
Priority date	Sep 16, 2010
Publication date	Jan 25, 2018
Grant date	—

Abstract

The invention relates to the detection of DHA and EPA. In a particular aspect, the invention relates to methods for detecting DHA and EPA by mass spectrometry and kits for carrying out such methods.

First claim

That which is claimed is:

1 . A method for determining the amount of one or more omega-3 fatty acids in a sample by mass spectrometry, the method comprising:
(i) ionizing said one or more omega-3 fatty acids from the sample to generate one or more ions detectable by mass spectrometry; (ii) determining the amount of said one or more omega-3 fatty acids ions by mass spectrometry; and (iii) relating the amount of DHA ions to the amount of one or more omega-3 fatty acids in the sample, wherein said one or more omega-3 fatty acids comprises docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA).

2 . The method of claim 1 , wherein said ionizing comprises atmospheric pressure chemical ionization (APCI).

3 . The method of claim 2 , wherein said APCI is in negative ionization mode.

4 . The method of claim 1 , wherein said ionizing comprises electrospray ionization (ESI).

5 . The method of claim 1 , wherein mass spectrometry comprises tandem mass spectrometry.

6 . The method of claim 1 , wherein said one or more ions comprise an ion with a mass to charge ratio (m/z) of 327.2±0.5.

7 . The method of claim 1 , wherein said one or more ions comprise an ion with a mass to charge ratio (m/z) of 301.2±0.5.

8 . The method of claim 1 , wherein said sample comprises human serum or plasma.

9 . The method of claim 1 , wherein said sample is subjected to a hydrolyzing agent prior to ionization.

10 . The method of claim 8 , wherein said hydrolyzing agent is an acid.

11 . The method of claim 1 , wherein said one or more omega-3 fatty acids is subjected to liquid/liquid extraction prior to ionization.

12 . The method of claim 1 , wherein said one or more omega-3 fatty acids is purified by liquid chromatography prior to ionization.

13 . The method of claim 12 , wherein said liquid chromatography comprises high performance liquid chromatography (HPLC).

14 . The method of claim 1 , wherein said method comprises determining the amount of an internal standard.

15 . The method of claim 14 , wherein said internal standard is a deuterated omega-3 fatty acid.

16 . The method of claim 15 , wherein said deuterated omega-3 fatty acid is DHA- 2 H 5 .

17 . The method of claim 15 , wherein said deuterated omega-3 fatty acid is EPA- 2 H 5 .

Assignees

• Quest Diagnostics Invest Inc

Inventors

• Goldman Scott M
• Neidich Julie A

Classifications

• Y10T436/25375

  Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] · CPC title

• H01J49/145

  using chemical ionisation · CPC title

• Y10T436/104165

  Lipid, cholesterol, or triglyceride standard or control · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• G01N30/7266

  by electric field, e.g. electrospray · CPC title