Novel metalloproteases

Patent metadata

Field	Value
Publication number	US-2018023068-A1
Application number	US-201715726719-A
Country	US
Kind code	A1
Filing date	Oct 6, 2017
Priority date	May 29, 2013
Publication date	Jan 25, 2018
Grant date	—

Abstract

Aspects of the present compositions and methods relate to novel metalloproteases polynucleotides encoding the novel metalloprotease, compositions and methods for use thereof.

First claim

1 . A polypeptide comprising an amino acid sequence having at least 60% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 3, 6, 9 and 13.

2 . The polypeptide of claim 1 , wherein said polypeptide has at least 80% sequence identity to the amino acid sequence selected from the group consisting of SEQ ID NO: 3, 6, 9 and 13.

3 . The polypeptide of any of claim 1 or 2 , wherein said polypeptide has at least 95% sequence identity to the amino acid sequence selected from the group consisting of SEQ ID NO: 3, 6, 9 and 13.

4 . The polypeptide of any of the above claims, wherein said amino acid sequence is the amino acid sequence selected from the group consisting of SEQ ID NO: 3, 6, 9 and 13.

5 . The polypeptide of any of the above claims, wherein said polypeptide is derived from a member of the Actinomycetales.

6 . The polypeptide of any of the above claims, wherein said polypeptide is derived from a member of the Streptomyces . spp.

7 . The polypeptide of claim 6 , wherein said is Streptomyces rubiginosus.

8 . The polypeptide of claim 6 , wherein said is Streptomyces lividans.

9 . The polypeptide of claim 6 , wherein said is Streptomyces scabiei.

10 . The polypeptide of any of the above claims, wherein said polypeptide has protease activity.

11 . The polypeptide of claim 10 , wherein said protease activity comprises casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis.

12 . The polypeptide of any of the above claims, wherein said polypeptide retains at least 50% of its maximal activity between pH 4.5 and 9.5.

13 . The polypeptide of any of the above claims, wherein said polypeptide retains at least 50% of its maximal activity between 30° C. and 65° C.

14 . The polypeptide of any of the above claims, wherein said polypeptide has cleaning activity in a detergent composition.

15 . The polypeptide of claim 14 , wherein said detergent composition is an ADW detergent composition.

16 . The polypeptide of claim 14 , wherein said detergent composition is a laundry detergent composition.

17 . The polypeptide of claim 16 , wherein said detergent composition is a liquid laundry detergent composition.

18 . The polypeptide of claim 16 , wherein said detergent composition is a powder laundry detergent composition.

19 . The polypeptide of claim 14 , wherein said detergent composition comprises a bleach component.

20 . The polypeptide of any of the above claims, wherein said polypeptide is a recombinant polypeptide.

21 . A composition comprising the polypeptide of any of the above claims.

22 . The composition of claim 21 , wherein said composition is a cleaning composition.

23 . The composition of claim 22 , wherein said composition is a detergent composition.

24 . The composition of claim 23 , wherein said detergent composition is selected from the group consisting of a laundry detergent, a fabric softening detergent, a dishwashing detergent, and a hard-surface cleaning detergent.

25 . The composition of any of claims 21 to 24 , wherein said composition further comprises a surfactant.

26 . The composition of claim 25 , wherein said surfactant is selected from the group consisting of an anionic surfactant, a cationic surfactant, a zwitterionic surfactant, a ampholytic surfactant, a semi-polar non-ionic surfactant, and a combination thereof.

27 . The composition of claim 25 , wherein said surfactant is an ionic surfactant.

28 . The composition of claim 25 , wherein said surfactant is a non-ionic surfactant.

29 . The composition of any of claims 21 - 28 , wherein said composition further comprises at least one calcium ion and/or zinc ion.

30 . The composition of any of claims 21 - 29 , wherein said composition further comprises at least one stabilizer.

31 . The composition of any of claims 21 - 30 , wherein said composition comprises from about 0.001 to about 0.1 weight % of said polypeptide.

32 . The composition of any of claims 21 - 31 , further comprising at least one bleaching agent.

33 . The composition of any of claims 21 - 32 , wherein said cleaning composition is phosphate-free.

34 . The composition of any of claims 21 - 32 , wherein said cleaning composition contains phosphate.

35 . The composition of any of claims 21 - 34 , further comprising at least one adjunct ingredient.

36 . The composition of any of claims 21 - 35 , wherein said composition is a granular, powder, solid, bar, liquid, tablet, gel, or paste composition.

37 . The composition of any of claims 21 - 36 , further comprising one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, additional metallopotease enzymes and combinations thereof.

38 . The composition of any of claims 21 - 37 , wherein said composition is formulated at a pH of from about 5.5 to about 8.5.

39 . A method for the pretreatment of animal feed comprising treating an animal feed pre-product with the polypeptide of any one of claims 1 - 20 .

40 . A method of cleaning, comprising contacting a surface or an item with a cleaning composition comprising the polypeptide of any one of claims 1 - 20 .

41 . A method of cleaning comprising contacting a surface or an item with the composition of any one of claims 21 - 38 .

42 . The method of claim 40 or 41 , further comprising rinsing said surface or item after contacting said surface or item, respectively, with said composition.

43 . The method of any one of claims 40 - 42 , wherein said item is dishware.

44 . The method of any one of claims 40 - 42 , wherein said item is fabric.

45 . The method of any one of claims 40 - 44 , further comprising the step of rinsing said surface or item after contacting said surface or item with said composition.

46 . The method of claim 45 , further comprising the step of drying said surface or item after said rinsing of said surface or item.

47 . A method of cleaning a surface or item, comprising: providing the composition of any of claims 22 - 37 and a surface or item in need of cleaning; and contacting said composition with said surface or item in need of cleaning under conditions suitable for the cl

Assignees

• Danisco Us Inc

Inventors

• Babe Lilia M
• Ghirnikar Roopa
• Goedegebuur Frits
• Gu Xiaogang
• Kolkman Marc
• Yao Jian
• Yu Shukun

Classifications

• C12N9/52 Primary

  derived from bacteria {or Archaea} · CPC title

• C12Y304/24

  Metalloendopeptidases (3.4.24) · CPC title

• A23K10/14

  Pretreatment of feeding-stuffs with enzymes · CPC title

• C11D3/386

  Preparations containing enzymes {, e.g. protease or amylase} · CPC title

• A23K20/189

  Enzymes · CPC title