Device for solid phase extraction and method for use thereof

Patent metadata

Field	Value
Publication number	US-2018001229-A1
Application number	US-201515536506-A
Country	US
Kind code	A1
Filing date	Dec 16, 2015
Priority date	Dec 17, 2014
Publication date	Jan 4, 2018
Grant date	—

Abstract

Disclosed is a device for a solid phase extraction comprising two or more of the sorbents to remove phospholipids and salts from a sample, to thereby eliminate matrix effects during mass spectrometry analysis. In particular, the sorbents includes at least one sorbent which is water-wettable and contains at least one hydrophobic component and at least one hydrophilic component and at least one of sorbent having a specific affinity for a matrix interference like phospholipids. Further disclosed is a method using the device of the present invention.

First claim

1 . A separation device comprising two or more sorbents wherein:
the matrix interferences retained on one of the sorbents is substantially removed by the other sorbents as compared to a separation device comprising only one sorbent; the flowrate of the sorbents is substantially improved as compared to a separation device comprising only one sorbent; or the backpres sure of the sorbents is substantially reduced as compared to a separation device comprising only one sorbent.

2 . The separation device of claim 1 , wherein at least one sorbent contains at least one hydrophobic component and at least one hydrophilic component.

3 . The separation device of claim 2 , wherein the sorbent that contains at least one hydrophobic component and at least one hydrophilic component is water-wettable.

4 . The separation device of claim 1 , wherein at least one of the sorbents has retention for analytes having a log P of 0.05 or greater in aqueous solutions.

5 . The separation device of claim 4 , wherein the remainder of the sorbents, in total, have a specific affinity for matrix interferences in greater than 40% organic solvent solutions.

6 . The separation device of claim 1 , wherein at least one sorbent has a specific affinity to phospholipids.

7 . The separation device of claim 1 , wherein the sorbents are selected from the group consisting of a silica, a modified silica, an alumina, an modified alumina, titanium and zirconium oxides, graphitized carbon or activated carbon, magnesium-silica, a modified polymer and combinations thereof.

8 . The separation device of claim 1 , wherein the sorbents are layered.

9 . The separation device of claim 8 , wherein the top layer is water-wettable.

10 . The separation device of claim 9 , wherein the top layer is the sorbent containing at least one hydrophobic component and at least one hydrophilic component.

11 . The separation device of claim 1 , wherein the sorbents are mixed in a single layer.

12 . The separation device of claim 11 , wherein the single layer is water-wettable.

13 . The separation device of claim 11 , wherein a sorbent containing at least one hydrophobic component and at least one hydrophilic component and a sorbent having a specific affinity to phospholipid are mixed at a weight ratio of about 1:2.

14 . (canceled)

15 . (canceled)

16 . (canceled)

17 . (canceled)

18 . (canceled)

19 . (canceled)

20 . The separation device of claim 3 , wherein the sorbent is a reversed-phase water-wettable sorbent.

21 . The separation device of claim 20 , wherein the sorbent is OASIS® HLB.

22 . The separation device of claim 5 , wherein the sorbent is a silica containing a modified or unmodified octadecyl (C 18 ) or C 8 to a C 30 alkyl component.

23 . (canceled)

24 . A chromatographic column, comprising:
a column having a cylindrical interior for accepting a sorbent bed; and at least two or more sorbents.

25 . The chromatographic column of claim 24 , wherein at least one sorbent contains at least one hydrophobic component and at least one hydrophilic component.

26 . The chromatographic column of claim 25 , wherein the sorbent that contains at least one hydrophobic component and at least one hydrophilic component is water-wettable.

27 . The chromatographic column of claim 24 , wherein at least one of the sorbents has retention for analytes having a log P of 0.05 or greater in aqueous solutions.

28 . The chromatographic column of claim 27 , wherein the remainder of the sorbents, in total, have a specific affinity for matrix interferences in greater than 40% organic solvent solutions.

29 . The chromatographic column of claim 24 , wherein at least one sorbent has a specific affinity to phospholipids.

30 . The chromatographic column of claim 24 , wherein the sorbents are selected from the group consisting of a silica, a modified silica, an alumina, an modified alumina, titanium and zirconium oxides, graphitized carbon or activated carbon, magnesium-silica, a modified polymer and combinations thereof.

31 . The chromatographic column of claim 24 , wherein the sorbents are layered.

32 . The chromatographic column of claim 31 , wherein a top layer is water wettable

33 . The chromatographic column of claim 32 , wherein the top layer is the sorbent containing at least one hydrophobic component and at least one hydrophilic component.

34 . The chromatographic column of claim 24 , wherein the sorbents are mixed in a single layer.

35 . The chromatographic column of claim 34 , wherein the single layer is water wettable.

36 . The chromatographic column of claim 34 , wherein a sorbent containing at least one hydrophobic component and at least one hydrophilic component and a sorbent having a specific affinity to phospholipid are mixed at a weight ratio of about 1:2.

37 . (canceled)

38 . (canceled)

39 . (canceled)

40 . (canceled)

41 . (canceled)

42 . (canceled)

43 . The chromatographic column of claim 25 , wherein the sorbent is a reversed-phase water-wettable sorbent.

44 . The chromatographic column of claim 43 , wherein the sorbent is OASIS® HLB.

45 . The chromatographic column of claim 29 , wherein the sorbent is a silica containing modified or unmodified octadecyl (C 18 ) or C 8 to C 30 alkyl component.

46 . A chromatographic device, comprising:
a column having a cylindrical interior for accepting a sorbent bed; and at least two or more sorbents.

47 . The chromatographic device of claim 46 , wherein that at least one sorbent contains at least one hydrophobic component and at least one hydrophilic component and at least one sorbent has a specific affinity to phospholipids are included in the sorbent bed, wherein the sorbent that contains at least one hydrophobic component and at least one hydrophilic component is water-wettable

48 . The chromatographic device of claim 46 , wherein the sorbents are layered.

49 . The chromatographic device of claim 46 , wherein a top layer is water wettable.

50 . The chromatographic device of claim 49 , wherein the top layer comprises a sorbent containing at least one hydrophobic component and at least one hydrophilic component.

51 . The chromatographic device of claim 46 , wherein the sorbents are mixed in a single layer.

52 . The chromatographic device of claims 46 , wherein the sorbent containing at least one hydrophobic component and at least one hydrophilic component and the sorbent having a specific affinity to phospholipid are mixed at a weight ratio of about 1:2.

53 . (canceled)

54 . (canceled)

55 . (canceled)

56 . (canceled)

57 . (canceled)

58 . (canceled)

59 . The chromatographic device of claim 46 , wherein the sorbent containing at least one hydrophobic component and at least one hydrophilic

Assignees

• Waters Technologies Corp

Inventors

• Iraneta Pamela C
• Zhang Xin
• Marszalkowski Jr Frank John

Classifications

• B01D15/3847 Primary

  Multimodal interactions · CPC title

• B01J20/288

  Polar phases · CPC title

• B01J20/3285

  Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic · CPC title

• G01N2030/009

  Extraction · CPC title

• B01J20/3293

  Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers · CPC title