High Capacity Redox Electrodes and Their Use in Cell Lysis

Patent metadata

Field	Value
Publication number	US-2017363561-A1
Application number	US-201515532233-A
Country	US
Kind code	A1
Filing date	Dec 3, 2015
Priority date	Dec 3, 2014
Publication date	Dec 21, 2017
Grant date	—

Abstract

The present disclosure relates to the manufacture and use of redox electrodes and their use in cell lysis. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 Volts.

First claim

1 . An oxidation-reduction (redox) electrode, comprising:
a support substrate comprising one or more of particles or fibers; and a redox polymer combined with the support substrate particles or fibers and having a thickness greater than 1 micron with respect to the surface of the support substrate.

2 . The redox electrode of claim 1 , wherein the redox polymer comprises a conjugated redox polymer.

3 . The redox electrode of claim 2 , wherein the conjugated redox polymer comprises poly(3,4-ethylenedioxythiophene) (PEDOT).

4 . The redox electrode of claim 2 , wherein the conjugated redox polymer comprises one or more of a conjugated polythiophene polymer, a conjugated polyaniline polymer, or a conjugated polypyrrole polymer.

5 . The redox electrode of claim 1 , wherein the support substrate comprises textile fibers, a plastic substrate, or a polymer substrate.

6 . The redox electrode of claim 1 , wherein the support substrate comprises cellulose fibers.

7 . The redox electrode of claim 1 , wherein the redox polymer comprises a supporting counter-ion or anionic copolymer.

8 . The redox electrode of claim 7 , wherein the supporting counterion or anionic copolymer comprises polystyrene sulfonate (PSS).

9 . The redox electrode of claim 1 , wherein the redox polymer comprises poly(3,4-ethylenedioxythiophene) (PEDOT): polystyrene sulfonate (PSS).

10 . The redox electrode of claim 1 , further comprising an ionic cross-linking agent.

11 . The redox electrode of claim 10 , wherein the ionic cross-linking agent comprises MgSO 4 .

12 . The redox electrode of claim 1 , further comprising an ionomer coating.

13 . The redox electrode of claim 1 , further comprising:
a metallic wire, foil, or mesh about which the support substrate and redox polymer are disposed.

14 . A method for manufacturing a redox electrode, comprising:
blending an aqueous dispersion of a redox polymer with cellulose fibers to form a slurry; solution casting the slurry into one or more molds each having a form factor corresponding to the redox electrode to form a respective redox electrode in each mold; and recovering the respective redox electrode from each mold.

15 . The method of claim 14 , wherein the aqueous dispersion comprises a PEDOT:PSS aqueous dispersion.

16 . The method of claim 14 , wherein the slurry is 5-25 wt % PEDOT:PSS.

17 . The method of claim 14 , wherein the slurry is 14-18 wt % PEDOT:PSS.

18 . The method of claim 14 , further comprising:
cross-linking the recovered redox electrodes using an MgSO4 aqueous solution.

19 . A method for manufacturing a redox electrode, comprising:
dip coating a cellulose paper substrate in an aqueous dispersion of a redox polymer one or more times; and forming the redox electrode using the dip coated cellulose paper.

20 . The method of claim 19 , wherein the aqueous dispersion comprises a PEDOT:PSS aqueous dispersion.

21 . A method for manufacturing a redox electrode, comprising:
solution casting an aqueous dispersion of a redox polymer onto a cellulose paper substrate; and forming the redox electrode using the solution cast onto the cellulose paper.

22 . The method of claim 21 , further comprising:
mounting the cellulose paper substrate in an aluminum frame at between about 100° C. to about 150° C. prior to solution casting.

23 . Apparatus for lysing a cell in order to release DNA from said cell, the apparatus comprising a volume from holding one or more cells in suspension, and an electrode pair capable of providing a potential difference across the volume suitable for rupturing the wall of said cell(s), at least one of said electrode pair being formed in accordance with claim 1 .

24 . A method for cell lysis, the method comprising the steps of:
a) introducing one or more cells into a volume, said volume including an electrode pair, at least one of said electrode pair being formed in accordance with claim 1 ; and b) providing a potential difference across the electrode pair of sufficient amplitude for rupturing the wall of said cell(s) and preferably in the order of 25 to 2000V/cm, preferably 25 to 1000V/cm.

Assignees

• Gen Electric

Inventors

• Peters Andrea Jeannine
• Stecher Thomas Elliot
• Galligan Craig Patrick
• Puleo Christopher Michael
• Lenigk Ralf

Classifications

• Y02E60/13

  Energy storage using capacitors · CPC title

• H01G9/042

  characterised by the material (H01G11/22 takes precedence) · CPC title

• H01G11/28

  arranged or disposed on a current collector; Layers or phases between electrodes and current collectors, e.g. adhesives · CPC title

• H01M4/602

  Polymers · CPC title

• H01G11/48

  Conductive polymers · CPC title