Nucleic acid analysis device

Patent metadata

Field	Value
Publication number	US-2017362634-A1
Application number	US-201415533087-A
Country	US
Kind code	A1
Filing date	Dec 26, 2014
Priority date	Dec 26, 2014
Publication date	Dec 21, 2017
Grant date	—

Abstract

The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.

First claim

1 .- 8 . (canceled)

9 . A nucleic acid analysis device comprising:
a flow cell for use in analysis of nucleic acid having a substrate for use in analysis of nucleic acid,
the substrate for use in analysis of nucleic acid having a plurality of analysis areas partitioned on the substrate to perform measurement by sequentially changing each analysis area,
the analysis area has an absorbent portion capable of absorbing a DNA fragment or a vector where the DNA fragment is borne and a non-absorbent portion other than the absorbent portion,
at least a part of the non-absorbent portion being provided with a marker portion having a predetermined shape to compute a position of the analysis area;
a circulation unit that circulates a fluid in a flow passage of the flow cell for use in analysis of nucleic acid; a temperature control unit that controls a reactive temperature of the DNA fragment; an irradiation unit that irradiates excitation light onto an analysis area serving as an analysis target through the light-transmitting cover; a detection unit that detects fluorescence emitted from the DNA fragment by irradiating the excitation light using the light-transmitting cover and detects a position of the marker portion in the analysis area from the detected fluorescence; and a carriage unit that carries the flow cell for use in analysis of nucleic acid and shifts the analysis area to a predetermined position by delivering the flow cell for use in analysis of nucleic acid with respect to the marker portion.

10 . The nucleic acid analysis device according to claim 9 , wherein movement to a predetermined position using the carriage unit in second and subsequent cycles is performed in an initial analysis area analyzed at least in the first cycle.

11 . The nucleic acid analysis device according to claim 9 , wherein the carriage unit has an XY-stage capable of delivering the flow cell for use in analysis of nucleic acid in each of X-axis and Y-axis directions coplanarly perpendicular to each other and a driving motor that drives the XY-stage.

Assignees

• Hitachi High Tech Corp

Inventors

• Ota Yuichiro
• Shoji Tomohiro
• Yokoyama Toru
• Narahara Masatoshi

Classifications

• C12Q1/68 Primary

  involving nucleic acids · CPC title

• G01N33/53

  Immunoassay; Biospecific binding assay; Materials therefor · CPC title

• G01B11/00

  Measuring arrangements characterised by the use of optical techniques · CPC title

• G01N37/00

  Details not covered by any other group of this subclass · CPC title

• G01N2021/6439

  with indicators, stains, dyes, tags, labels, marks · CPC title