Oyster peptide capable of enhancing sexual function, and preparation method and application thereof

Patent metadata

Field	Value
Publication number	US-2017362629-A1
Application number	US-201615542743-A
Country	US
Kind code	A1
Filing date	Aug 30, 2016
Priority date	Nov 5, 2015
Publication date	Dec 21, 2017
Grant date	—

Abstract

An oyster peptide capable of enhancing a sexual function, and a preparation method and application thereof are provided. In the method, oyster meat is pre-treated by using calcium salt before enzymatic hydrolysis, so as to activate and release endogenous enzymes of oysters, such that enzymatic preparations consumed in subsequent enzymatic hydrolysis can be reduced.

First claim

1 . A method for preparing a sexual function-improving oyster peptide, comprising:
1) grinding oyster flesh, calcium salt and water to obtain oyster flesh pulp; 2) enzymatic hydrolyzing oyster flesh pulp, centrifuging and collecting the supernatant to obtain an oyster enzyme-hydrolyzed raw solution; 3) decoloring the oyster enzyme-hydrolyzed raw solution and removing impurities to obtain a fine oyster peptide solution; and 4) concentrating and spay drying the fine oyster peptide solution to obtain the oyster peptide.

2 . The method according to claim 1 , wherein the calcium salt is food grade calcium chloride, calcium lactate, calcium carbonate, calcium hydrogen phosphate or calcium citrate.

3 . The method according to claim 1 , wherein the amount of the calcium salt in step 1) is 0.1%˜0.3% of the oyster flesh mass.

4 . The method according to claim 1 , wherein the step 2) comprises:
enzymatic hydrolyzing the oyster flesh pulp by neutral proteases or alkaline proteases; adding flavourzyme for further hydrolyzation; inactivating enzymes after enzymatic hydrolyzation; and centrifuging and collecting the supernatant to obtain an oyster enzyme-hydrolyzed raw solution.

5 . The method according to claim 4 , wherein the step 2) comprises:
stirring the oyster flesh pulp at 35° C.˜45° C. for 1 h˜2 h; adding neutral protease or alkaline protease; adjusting the pH and heat to 50° C.˜60° C. to continue hydrolysis for 5 h˜8 h; adjusting the pH to 5.0 to 5.5 and adding flavourzyme to continue hydrolysis at 50° C.˜60° C.; inactivating the enzymes 2 h—3 h after the hydrolysis; and centrifuging and collecting the supernatant to obtain an oyster enzyme-hydrolyzed raw solution.

6 . The method according to claim 4 , wherein the neutral protease is one or more of papain, bromelain, animal proteolytic enzyme and complex protease Protamex; the alkaline protease is one or more of Alcalase alkaline protease, FoodPro Alkaline Protease alkaline protease and trypsin.

7 . The method according to claim 4 , wherein the amount of the neutral protease or the alkaline protease is 0.3‰˜0.8‰ of the oyster flesh pulp mass.

8 . The method according to claim 4 , wherein the amount of the flavourzyme is 0.5‰˜1.0‰ of the oyster flesh pulp mass.

9 . An oyster peptide prepared by the method according to claim 1 .

10 . A method for improving sexual function and/or antioxidant in a subject in need thereof comprising administering to the subject the oyster peptide according to claim 9 .

Assignees

• Infinitus China Co Ltd

Inventors

• Xiao Xiaochun
• Zhai Xufeng
• Guo Xiaolei
• Lou Yongjun
• Ma Chung Wah

Classifications

• C12P21/06 Primary

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title

• C07K14/43509

  from crustaceans · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title