Nanowire-Coated Microdevice and Method of Making and Using the Same

Patent metadata

Field	Value
Publication number	US-2017360717-A1
Application number	US-201515536071-A
Country	US
Kind code	A1
Filing date	Dec 11, 2015
Priority date	Dec 15, 2014
Publication date	Dec 21, 2017
Grant date	—

Abstract

A microdevice containing a plurality of nanowires on a biocompatible surface, and methods of making and using the same are provided. Aspects of the present disclosure include forming a plurality of microdevices on a substrate where each microdevice includes a plurality of nanowires. The nanowires may be loaded with an active agent by disposing the active agent onto the surface of the nanowires. Also provided herein are kits that include the subject microdevices.

First claim

What is claimed is:

1 . A method of forming a plurality of nanowires on a biocompatible surface, comprising:
a) depositing onto a surface of a first biocompatible polymeric layer a second biocompatible polymeric layer; b) contacting a surface of the second biocompatible polymeric layer with a nanoporous membrane; and c) forming the second biocompatible polymeric layer into a plurality of nanowires using the nanoporous membrane as a template.

2 . The method of claim 1 , wherein the forming the second biocompatible polymeric layer into a plurality of nanowires using the nanoporous membrane as a template comprises extruding the second biocompatible polymeric layer through the nanoporous membrane using heat.

3 . The method according to any one of claim 1 or 2 , wherein the method further comprises dissolving the nanoporous membrane after forming the second biocompatible polymeric layer into a plurality of nanowires.

4 . The method of claim 3 , wherein the dissolving comprises dissolving the nanoporous membrane in an alkaline solution.

5 . The method of any of claims 1 to 4 , wherein the depositing step a) comprises contacting a heated first biocompatible polymeric layer with the second biocompatible polymeric layer.

6 . The method of any of claims 1 to 5 , wherein the first biocompatible polymeric layer comprises a biocompatible polymer selected from the group consisting of: polymethyl methacrylate (PMMA), collagen, PLA, PGA, poly(anhydrides), poly(hydroxy acids), poly(lactic-co-glycolic acid) (PLGA), and chitosan.

7 . The method of any of claims 1 to 6 , wherein the second biocompatible polymeric layer comprises a biocompatible polymer selected from the group consisting of: polycaprolactone (PCL), gelatin, agarose, poly(anhydrides), poly(hydroxy acids), poly(propylfumerates), poly(lactic-co-glycolic acid) (PLGA), and chitosan.

8 . The method of any of claims 1 to 7 , wherein the nanoporous membrane is an anodized metal oxide membrane or a nanoporous silica membrane.

9 . The method of claim 8 wherein the anodized metal oxide comprises aluminum, tin or titanium.

10 . The method of claim 9 wherein the nanoporous membrane is a nanoporous anodized aluminum oxide (AAO) membrane.

11 . The method of any of claims 1 to 10 , wherein the method comprises providing the first biocompatible polymeric layer on a substrate prior to step a).

12 . The method of claim 11 , wherein the method comprises creating a micropattern in the first biocompatible polymeric layer prior to step a).

13 . The method of any of claims 1 to 12 , wherein the method comprises creating a micropattern in the nanoporous membrane prior to step b).

14 . The method of any one of claims 12 and 13 , wherein creating the micropattern comprises using photolithography.

15 . The method of any one of claims 11 - 14 , wherein the substrate is a silicon wafer.

16 . The method of any of claims 1 to 15 , wherein the first polymeric layer has an average thickness in the range of 1 to 100 μm.

17 . The method of any of claims 1 to 16 , wherein the second biocompatible polymeric layer has an average thickness in the range of 1 to 100 μm.

18 . The method of any of claims 1 to 17 , wherein the average diameter of the pores of the nanoporous membrane is in the range of 10 to 500 nm.

19 . A device comprising a first biocompatible layer, a second biocompatible layer disposed on a surface of the first biocompatible layer, the second biocompatible layer comprising a plurality of nanowires formed from the second biocompatible layer, where the nanowires are substantially perpendicular to the surface of the first biocompatible layer.

20 . A device comprising a substrate comprising a micropattern of a plurality of nanowire devices, wherein each of the nanowire devices comprises a first biocompatible layer disposed on a surface of the substrate, a second biocompatible layer disposed on a surface of the first biocompatible layer, the second biocompatible layer comprising a plurality of nanowires formed from the second biocompatible layer, wherein the nanowires are substantially perpendicular to the surface of the first biocompatible layer.

21 . The device of any one of claims 19 - 20 , wherein the nanowires have an average length of 1 to 100 μm.

22 . The device of any one of claims 19 - 21 , wherein the nanowires have an average diameter of 100 to 500 nm.

23 . The device of any of claims 19 to 22 , wherein an active agent is disposed on the plurality of nanowires.

24 . A method of loading an active agent on a device comprising a plurality of nanowire devices, the method comprising:
contacting a device of any one of claims 20 - 23 with a solution comprising an active agent, wherein the contacting comprises contacting the plurality of the nanowire devices thereby loading the microdevice comprising a plurality of nanowires on a biocompatible surface with an active agent.

25 . The method of claim 24 , wherein the method comprises drying the microdevice.

26 . The method according to claim 25 , wherein the drying comprises inverting the microdevice such that the biocompatible surface comprising the nanowires substantially faces down.

27 . A method of delivering an active agent to a mucosal surface, comprising:
contacting a plurality of nanowires of a microdevice comprising the plurality of nanowires on a biocompatible surface with a mucosal surface, wherein an active agent is disposed on the plurality of nanowires.

Assignees

• Univ California

Inventors

• Desai Tejal A
• Dinakar Hariharasudhan Chirra
• Fox Cade B

Classifications

• B81B2207/056

  of static structures · CPC title

• A61L31/16

  Biologically active materials, e.g. therapeutic substances {(A61L31/047 takes precedence)} · CPC title

• B81C1/00031

  Regular or irregular arrays of nanoscale structures, e.g. etch mask layer (photomechanical, e.g. photolithographic, production of textured or patterned surfaces G03F7/00; lithographic processes for making patterned surfaces using printing and stamping G03F7/0002) · CPC title

• A61K47/34

  Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers (A61K47/10 takes precedence) · CPC title

• A61K9/70 Primary

  Web, sheet or filament bases {; Films; Fibres of the matrix type containing drug (hollow drug-filled fibres A61K9/0092)} · CPC title