Liquid chromatograph and method for correcting detector output value fluctuation of liquid chromatograph

Patent metadata

Field	Value
Publication number	US-2017356887-A1
Application number	US-201715617790-A
Country	US
Kind code	A1
Filing date	Jun 8, 2017
Priority date	Jun 10, 2016
Publication date	Dec 14, 2017
Grant date	—

Abstract

A minimum peak is determined from analysis results, a correction wavelength at which an S/N ratio of the minimum peak is greatest is determined, and the determined correction wavelength is used to execute correction of the minimum peak. A plurality of detector output value correction method are registered in a processor, correction method is selected from default correction method or from among a plurality of preset correction methods according to an object to perform correction.

First claim

What is claimed is:

1 . A method for correcting detector output value of a liquid chromatograph, the method comprising:
selecting an analysis condition for using a liquid chromatograph, the analysis condition being selected from preset recipes that are registered in advance according to an examination object, the analysis condition being a condition to be set with respect to devices included in the liquid chromatograph such as a pump, an auto sampler, a column oven, and a detector, starting analysis according to the analysis condition that is selected in the previous step, measuring a characteristic of a sample using the detector, the characteristic including at least one of an absorbency and a fluorescence amount, and storing and displaying a signal strength of the detector indicating the characteristic of the sample in relation to time as analysis results; obtaining a chromatogram; recognizing peaks included in the chromatogram; determining a most minute peak of the signal strength of the analysis results in peaks which are recognized as a correction target peak; determining correction candidate wavelengths with respect to the correction target peak; performing a correction process on detector output value fluctuation based on correction method which is selected from among a plurality of preset correction methods; and outputting the analysis conditions, the correction condition, and corrected results of the characteristic of the sample.

2 . The method of claim 1 further comprising:
selecting one wavelength of a correction wavelength at which an S/N ratio, which is a ratio between a detector output signal of the correction target peak and a detector output signal in the correction wavelength, is greatest and correcting the detector output signal of the correction target peak.

3 . The method of claim 1 further comprising:
selecting one of a correction wavelength at which reproducibility of an S/N ratio is most favorable and correcting a detector output signal of the correction target peak.

4 . The method of claim 1 further comprising:
selecting a plurality of correction wavelengths at which reproducibility of an S/N ratio is most favorable and correcting a detector output signal of the correction target peak using an average value of the detector output values in the plurality of selected wavelengths.

5 . The method of claim 1 further comprising:
selecting each correction wavelength such that an S/N ratio of the correction target peak of each measurement target component is greatest and correcting a detector output signal of each of the correction target peaks.

6 . The method of claim 1 further comprising:
selecting a plurality of correction wavelengths at which reproducibility of an S/N ratio of the correction target peak of each measurement target component is most favorable and correcting a detector output signal of the correction target peak using an average value of the detector output values in the plurality of selected wavelengths.

7 . The method of claim 1 further comprising:
registering absorption wavelengths of a mobile phase which is used in liquid chromatography in advance as a database;
selecting correction wavelength from the database in accordance with the mobile phase being selected; and
correcting a detector output signal of the correction target peak using the selected correction wavelength.

8 . A liquid chromatograph comprising:
a pump that pumps a mobile phase; an auto sampler that injects a sample; a column oven that maintains a column that separates components at a constant temperature; a detector that detects separated components; a processor that selects a recipe during measurement from analysis conditions which are preset in advance, selects detector output value correction process method which is registered, and examines analysis results; a data storage that stores the analysis results and examination results; and a display unit that displays the analysis conditions, the analysis results, and the examination results, wherein the processor operates to perform a process comprising:
selecting analysis conditions from a recipe which is registered in advance according to an examination object;
performing analysis according to the selected analysis conditions;
measuring a characteristic of a sample, the characteristic including at least one of an absorbency and a fluorescence amount;
determining a signal strength of the detector such as the absorbency or the fluorescence amount of the sample as analysis results;
recognizing peaks included in the chromatogram;
setting a minimum peak from among the recognized peaks as a correction target peak;
selecting a correction candidate wavelength for the correction target peak;
selecting detector output value correction method which is registered in advance; and
performing a correction process based on the selected detector output value correction method.

9 . A liquid chromatograph comprising:
a pump that pumps a mobile phase; an auto sampler that injects a sample; a column oven that maintains a column that separates components at a constant temperature; a detector that detects separated components; a processor that selects a recipe during measurement from analysis conditions which are preset in advance, selects detector output value correction process method which is registered, and examines analysis results; a data storage that stores the analysis results and examination results; and a display unit that displays the analysis conditions, the analysis results, and the examination results, wherein the processor operates to perform a process comprising:
selecting analysis conditions from a recipe which is registered in advance according to an examination object;
performing analysis according to the selected analysis conditions;
measuring a characteristic of a sample, the characteristic including at least one of an absorbency and a fluorescence amount;
determining a signal strength of the detector such as the absorbency or the fluorescence amount of the sample as analysis results;
recognizing peaks included in the chromatogram;
setting a plurality of peaks from among the recognized peaks as a correction target peaks;
selecting a plurality of correction candidate wavelengths for the correction target peaks;
selecting detector output value correction method which is registered in advance; and
performing a correction process based on the selected detector output value correction method.

Assignees

• Hitachi High-Tech Science Corp

Inventors

• Yamamura Shuhei
• Akieda Daisuke
• Nakaogami Ayumi
• Shimizu Katsutoshi

Classifications

• G01N21/314 Primary

  with comparison of measurements at specific and non-specific wavelengths (dual wavelength spectrometry G01J3/427) · CPC title

• G01N30/34

  of fluid composition, e.g. gradient (G01N30/36 takes precedence) · CPC title

• G01N30/60 Primary

  Construction of the column · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N2030/027

  Liquid chromatography · CPC title