Methods and systems relating to dielectrophoretic manipulation of molecules

Patent metadata

Field	Value
Publication number	US-2017326558-A1
Application number	US-201715596655-A
Country	US
Kind code	A1
Filing date	May 16, 2017
Priority date	May 16, 2016
Publication date	Nov 16, 2017
Grant date	—

Abstract

There is described herein methods and devices for confining and/or manipulating molecules. At least one molecule is introduced into a fluidic chamber. The fluidic chamber is formed inside a device comprising at least one first electrode having a first surface spaced from at least one second electrode having a second surface facing the first surface. The at least one second electrode has a plurality of dielectric structures arranged to form openings along the second surface. At least one electrical signal is applied across the at least one first electrode and the at least one second electrode to generate a non-uniform electric field having electric field lines extending from the first surface of the at least one first electrode to the second surface of the at least one second electrode in the openings formed between the dielectric structures. The at least one electrical signal has a frequency level causing the at least one molecule to move inside the fluidic chamber in accordance with a predetermined movement.

First claim

1 . A confinement device for molecules comprising:
at least one first electrode having a first surface; at least one second electrode having a second surface spaced from the first surface of the first electrode and forming a fluidic chamber therebetween; and a plurality of dielectric structures on the second surface arranged to form openings along the second surface, wherein application of an electrical signal across the at least one first electrode and the at least one second electrode generates a non-uniform electric field having electric field lines extending from the first surface of the at least one first electrode to the second surface of the at least one second electrode in the openings formed between the dielectric structures.

2 . The device of claim 1 , wherein at least one of the at least one first electrode and the at least one second electrode comprises a matrix of electrodes.

3 . The device of claim 2 , wherein electrodes in the matrix of electrodes are individually connected to electrode pads for selective application of an electrical signal.

4 . The device of claim 1 , further comprising at least one spacer between the at least one first electrode and one of the at least one second electrode and outer ones of the plurality of dielectric structures.

5 . The device of claim 1 , wherein at least one of the at least one first electrode and the at least one second electrode is composed of an optically transparent indium tin oxide.

6 . The device of claim 1 , wherein the at least one second electrode has a third surface opposite to the second surface, and further comprising a substrate in contact with the third surface.

7 . The device of claim 1 , further comprising a casing surrounding the at least one first electrode and the at least one second electrode, the casing composed of a transparent, biologically inert material.

8 . The device of claim 1 , further comprising at least one port extending through one of the at least one first electrode and the at least one second electrode and in fluid communication with the fluidic chamber.

9 . The device of claim 1 , wherein the openings formed along the second surface of the second electrode by the dielectric structures are channels that extend at least partially across the second surface.

10 . The device of claim 1 , wherein the second surface of the at least one second electrode is coated with a layer of dielectric material.

11 . A method for manipulating molecules, the method comprising:
introducing at least one molecule into a fluidic chamber, the fluidic chamber formed inside a device comprising at least one first electrode having a first surface spaced from at least one second electrode having a second surface facing the first surface, the at least one second electrode having a plurality of dielectric structures arranged to form openings along the second surface; and applying at least one electrical signal across the at least one first electrode and the at least one second electrode to generate a non-uniform electric field having electric field lines extending from the first surface of the at least one first electrode to the second surface of the at least one second electrode in the openings formed between the dielectric structures, the at least one electrical signal having a frequency level causing the at least one molecule to move inside the fluidic chamber in accordance with a predetermined movement.

12 . The method of claim 11 , wherein applying the at least one electrical signal comprises selecting the frequency level to cause the at least one molecule to align with the electric field lines.

13 . The method of claim 11 , wherein applying the at least one electrical signal comprises selecting the frequency level to cause the at least one molecule to be driven and confined into the openings formed between the dielectric structures.

14 . The method of claim 11 , wherein applying the at least one electrical signal comprises selecting the frequency level to cause one of linearization and accumulation of the at least one molecule.

15 . The method of claim 11 , wherein applying the at least one electrical signal comprises selectively attracting and repelling the at least one molecule from regions of the fluidic chamber by varying the frequency level of the at least one electrical signal.

16 . The method of claim 11 , wherein applying the at least one electrical signal comprises applying multiple electrical signals to selectively displace different ones of the at least one molecule within the fluidic chamber.

17 . The method of claim 11 , wherein applying the at least one electrical signal comprises selectively applying the at least one electrical signal to different regions of the device to confine and release the at least one molecule as a function of a position in the fluidic chamber.

18 . The method of claim 11 , further comprising applying one of fluidic pressure and hydrodynamic pressure across the device to displace the at least one molecule within the fluidic chamber.

19 . The method of claim 18 , wherein applying one of fluidic pressure and hydrodynamic pressure across the device comprises first removing the at least one electrical signal to release the at least one molecule.

20 . The method of claim 11 , wherein applying at least one electrical signal comprises applying at least one first electrical signal to cause a dielectrophoretic force to act on the at least one molecule and applying at least one second electrical signal to cause an electrophoretic force to act on the at least one molecule.

21 . The method of claim 20 , wherein the at least one first electrical signal and the at least one second electrical signal are applied concurrently.

Assignees

• The Royal Institution For The Advancement Of Learning/Mcgill Univ

Inventors

• MAHSHID Sara
• Ahamed Mohammed Jalal
• Reisner Walter
• Sladek Robert

Classifications

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C5/026 Primary

  using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC] · CPC title

• B03C5/005

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• B03C5/02 Primary

  Separators · CPC title

• G01N27/44791

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title