Systems and methods for electrochemical triglycerides assays

Patent metadata

Field	Value
Publication number	US-2017299539-A1
Application number	US-201715485871-A
Country	US
Kind code	A1
Filing date	Apr 12, 2017
Priority date	Apr 13, 2016
Publication date	Oct 19, 2017
Grant date	—

Abstract

A system for the electrochemical detection of triglyceride levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides.

First claim

What is claimed as new and desired to be protected by Letters Patent of the United States is:

1 . A reagent mixture for an electrochemical triglyceride assay comprising:
a surfactant; a triglyceride hydrolyzing reagent; Glycerol kinase; Glycerol-3-phosphate dehydrogenase; a coenzyme; and a redox agent capable of being oxidized or reduced to form a product.

2 . The reagent mixture of claim 1 , wherein the triglyceride hydrolyzing reagent is lipoprotein lipase.

3 . The reagent mixture of claim 1 , wherein the coenzyme is nicotinamide adenine dinucleotide (NAD).

4 . The reagent mixture of claim 1 , wherein the surfactant includes a bile acid derivative.

5 . The reagent mixture of claim 1 , wherein the surfactant includes a salt of a bile acid derivative.

6 . The reagent mixture of claim 4 , wherein the bile acid derivative is selected from a list of bile acids consisting of 5-Cholenic acid-3β-ol; 5α-Cholanic acid-3α-ol-6-one 3-acetate methyl ester; 5β-Cholanic acid 3,7-dione methyl ester; 5β-Cholanic acid; 5β-Cholanic acid-3α,12α-diol 3-acetate methyl ester; Chenodeoxycholic acid; Cholic acid; Deoxycholic acid; Glycoursodeoxycholic acid; Hyodeoxycholic acid methyl ester; Hyodeoxycholic acid; Lithocholic acid; Sodium cholate hydrate; Sodium deoxycholate; Sodium glycochenodeoxycholate; Sodium taurochenodeoxycholate; Sodium taurocholate hydrate; Taurocholic acid sodium salt hydrate; and Ursodeoxycholic acid.

7 . The reagent mixture of claim 1 , further comprising a Diaphorase enzyme.

8 . A system for the electrochemical detection of triglyceride levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides.

9 . The system of claim 8 , wherein the coating includes:
a surfactant; a triglyceride hydrolyzing reagent; Glycerol kinase; Glycerol-3-phosphate dehydrogenase; a coenzyme; and a redox agent capable of being oxidized or reduced to form a product.

10 . The system of claim 9 , wherein the triglyceride hydrolyzing reagent is lipoprotein lipase.

11 . The system of claim 9 , wherein the coenzyme is nicotinamide adenine dinucleotide (NAD).

12 . The system of claim 9 , wherein the surfactant includes a bile acid derivative.

13 . The system of claim 7 , wherein the surfactant includes a salt of a bile acid derivative.

14 . A reagent mixture for an electrochemical triglyceride assay comprising:
a triglyceride hydrolyzing reagent; Glycerol kinase; Glycerol-3-phosphate dehydrogenase; a coenzyme; and a redox agent capable of being oxidized or reduced to form a product.

15 . The reagent mixture of claim 14 , further comprising a surfactant.

16 . The reagent mixture of claim 15 , wherein the triglyceride hydrolyzing reagent is lipoprotein lipase.

17 . The reagent mixture of claim 16 , wherein the coenzyme is nicotinamide adenine dinucleotide (NAD).

18 . The reagent mixture of claim 17 , wherein the surfactant includes a bile acid derivative.

19 . The reagent mixture of claim 17 , wherein the surfactant includes a salt of a bile acid derivative.

20 . A system for the electrochemical detection of triglyceride levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides; and an analyzer for receiving the test strip and including instructions stored on a non-transitory medium for applying a current or a voltage to the test strip and responsively determining an amount of triglycerides.

21 . The system of claim 20 , wherein the coating includes:
a surfactant; a triglyceride hydrolyzing reagent; Glycerol kinase; Glycerol-3-phosphate dehydrogenase; a coenzyme; and a redox agent capable of being oxidized or reduced to form a product.

22 . The system of claim 21 , wherein the triglyceride hydrolyzing reagent is lipoprotein lipase.

23 . The system of claim 21 , wherein the coenzyme is nicotinamide adenine dinucleotide (NAD).

24 . The system of claim 21 , wherein the surfactant includes a bile acid derivative.

25 . The system of claim 21 , wherein the surfactant includes a salt of a bile acid derivative.

26 . A method of detecting triglycerides, the method comprising:
providing an electrochemical test strip; placing the electrochemical test strip in an analyzer; placing a blood sample on the electrochemical test strip; measuring a current provided through the blood sample and the electrochemical test strip; and calculating a level of triglycerides with the analyzer based on the current.

27 . The method of claim 26 , wherein the test strip includes an electrode and a counter electrode, the electrode and counter electrode located in a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides.

28 . The system of claim 27 , wherein the coating includes:
a surfactant; a triglyceride hydrolyzing reagent; Glycerol kinase; Glycerol-3-phosphate dehydrogenase; a coenzyme; and a redox agent capable of being oxidized or reduced to form a product.

29 . The system of claim 28 , wherein the triglyceride hydrolyzing reagent is lipoprotein lipase.

30 . The system of claim 28 , wherein the coenzyme is nicotinamide adenine dinucleotide (NAD).

31 . The system of claim 28 , wherein the surfactant includes a bile acid derivative.

32 . The system of claim 28 , wherein the surfactant includes a salt of a bile acid derivative.

Assignees

• Polymer Technology Systems Inc

Inventors

• Hughes Gary L

Classifications

• G01N27/3272 Primary

  Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52) · CPC title

• G01N2333/902

  Oxidoreductases (1.) · CPC title

• G01N2333/92

  Triglyceride splitting, e.g. by means of lipase · CPC title

• G01N2333/91215

  with a definite EC number (2.7.1.-) · CPC title

• G01N2333/90203

  acting on the aldehyde or oxo group of donors (1.2) · CPC title