Systems and methods for electrochemical creatinine assays

Patent metadata

Field	Value
Publication number	US-2017284954-A1
Application number	US-201715475719-A
Country	US
Kind code	A1
Filing date	Mar 31, 2017
Priority date	Mar 31, 2016
Publication date	Oct 5, 2017
Grant date	—

Abstract

A system for the electrochemical detection of creatinine levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine.

First claim

What is claimed as new and desired to be protected by Letters Patent of the United States is:

1 . A system for the electrochemical detection of creatinine levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine.

2 . The system of claim 1 , wherein the reagent coating includes a surfactant, a binder, stabilizers, a buffer, sarcosine dehydrogenase, and potassium ferricyanide.

3 . The system of claim 1 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.

4 . The system of claim 3 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

5 . The system of claim 4 , wherein the reagent coating includes a surfactant and a buffer.

6 . The system of claim 5 , wherein the reagent buffer includes a binder and a stabilizer.

7 . The system of claim 1 , wherein the reagent coating includes creatinine deiminase and a mediator.

8 . The system of claim 7 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

9 . The system of claim 1 , wherein the reagent coating includes sarcosine oxidase and a mediator.

10 . The system of claim 9 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

11 . A system for the electrochemical detection of creatinine levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine; and an analyzer for receiving the test strip and including instructions stored on a non-transitory medium for applying a current to the test strip and responsively determining an amount of creatinine.

12 . The system of claim 11 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.

13 . The system of claim 12 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

14 . The system of claim 13 , wherein the reagent coating includes a surfactant and a buffer.

15 . The system of claim 14 , wherein the reagent buffer includes a binder and a stabilizer.

16 . The system of claim 11 , wherein the reagent coating includes creatinine deiminase and a mediator.

17 . The system of claim 16 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

18 . A system for the electrochemical detection of creatinine levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine; and an analyzer for receiving the test strip and including instructions stored on a non-transitory medium for determining a voltage of the test strip and responsively determining an amount of creatinine.

19 . The system of claim 18 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.

20 . The system of claim 19 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

21 . The system of claim 18 , wherein the reagent coating includes creatinine deiminase and a mediator.

22 . The system of claim 21 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

23 . A method of detecting creatinine, the method comprising:
providing an electrochemical test strip; placing the electrochemical test strip in an analyzer; placing a blood sample on the electrochemical test strip; measuring a current provided through the blood sample and the electrochemical test strip; and calculating a level of creatinine with the analyzer based on the current.

24 . The method of claim 18 , wherein the test strip includes an electrode and a counter electrode, the electrode and counter electrode located in a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine.

25 . The method of claim 24 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.

26 . The method of claim 25 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

27 . The method of claim 24 , wherein the reagent coating includes creatinine deiminase and a mediator.

28 . The method of claim 27 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.

Assignees

• Polymer Technology Systems Inc

Inventors

• Hughes Gary L
• Patwardhan Aniruddha
• Casterline Christine

Classifications

• G01N2333/978

  acting on carbon to nitrogen bonds other than peptide bonds (3.5) · CPC title

• C12Q1/004

  mediator-assisted · CPC title

• C12Y305/04021

  Creatinine deaminase (3.5.4.21) · CPC title

• C12Q1/005

  involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence) · CPC title

• C12Y105/08003

  Sarcosine dehydrogenase (1.5.8.3) · CPC title