Apparatus and method for manufacturing channel-coupled scaffold

Patent metadata

Field	Value
Publication number	US-2017253860-A1
Application number	US-201715438215-A
Country	US
Kind code	A1
Filing date	Feb 21, 2017
Priority date	Mar 3, 2016
Publication date	Sep 7, 2017
Grant date	—

Abstract

The present disclosure relates to an apparatus and a method for manufacturing a channel-coupled scaffold. The present disclosure provides a method for manufacturing a channel-coupled scaffold, which includes: (1) a step of compressing a first elastic substrate which includes a groove on the surface of the substrate to close the groove; (2) a step of loading a scaffold composition onto the closed groove; and (3) a step of restoring the elastic substrate. The present disclosure also provides an apparatus for manufacturing a channel-coupled scaffold, which includes: a first elastic substrate which includes a groove on the surface of the substrate and onto which a scaffold composition is loaded: and a compression module which compresses the width of the groove of the elastic substrate to close it. The apparatus or method may accumulate a microchannel controlling local mass transfer, and align a collagen fiber in the scaffold at the same time.

First claim

What is claimed is:

1 . A scaffold assembly comprising a first elastic substrate which comprises a groove on the surface of the substrate and onto which a scaffold composition is loaded, wherein the groove forms a channel when a scaffold composition is loaded thereonto.

2 . The scaffold assembly according to claim 1 , wherein the assembly further comprises a second elastic substrate which is located on the first elastic substrate and comprises a well penetrating the substrate, wherein the scaffold composition is loaded into the well.

3 . The scaffold assembly according to claim 1 , wherein the second elastic substrate further comprises an inlet and an outlet connected to both ends of the groove of the first elastic substrate.

4 . The scaffold assembly according to claim 1 , wherein the assembly further comprises a scaffold composition which is loaded onto the groove of the first elastic substrate.

5 . The scaffold assembly according to claim 1 , wherein the scaffold composition is one or more of a microfibril, a cell or a mixture thereof.

6 . The scaffold assembly according to claim 5 , wherein the microfibril is one or more synthetic polymer fiber selected from a group consisting of nylon, polyacrylic acid, polycarbonate, polyurethane, poly(ethylene-vinyl acetate), polystyrene, polyvinyl alcohol, cellulose acetate and polyethylene oxide or one or more natural polymer fiber selected from a group consisting of elastin, gelatin, fibrinogen, fibrin, alginate, cellulose, silk fibroin, chitosan, laminin, actin and collagen.

7 . The scaffold assembly according to claim 5 , wherein the cell is a neuron, a glial cell, a muscle cell, a solid cancer cell, a mesenchymal stem cell or a fibroblast.

8 . The scaffold assembly according to claim 1 , wherein the elastic substrate comprises one or more elastomer selected from a group consisting of natural polyisoprene, synthetic polyisoprene, polybutadiene, chloroprene rubber, butyl rubber, styrene-butadiene rubber, nitrile rubber, ethylene propylene rubber, ethylene propylene diene rubber, epichlorohydrin rubber, polyacrylic rubber, silicone rubber, fluorosilicone rubber, a fluoroelastomer, polyether block amide, chlorosulfonated polyethylene, ethylene-vinyl acetate and polydimethylsiloxane.

9 . An apparatus for manufacturing a channel-coupled scaffold, which comprises:
the scaffold assembly according to claim 1 ; and a compression module which compresses the width of the groove of the first elastic substrate to close it.

10 . The apparatus according to claim 9 , wherein the compression module comprises: a compression plate which contacts with two parallel sides of the elastic substrate; a compressor which compresses the elastic substrate by operating the compression plate; and a controller which controls the movement of the compressor.

11 . The apparatus according to claim 10 , wherein the controller compresses the width of the elastic substrate by 5-80% along a direction perpendicular to the groove by operating the compressor; and/or closes 30-100% of the width of the groove by operating the compressor.

12 . A method for manufacturing a channel-coupled scaffold using the apparatus according to claim 9 , which comprises:
(1) compressing a first elastic substrate which comprises a groove on the surface of the substrate and onto which a scaffold composition is loaded to close the groove; (2) loading a scaffold composition onto the closed groove; and (3) restoring the elastic substrate.

13 . The method according to claim 12 , which further comprises, before (1), (1)′ coating the surface of the elastic substrate with an adhesive.

14 . The method according to claim 13 , wherein the adhesive is one or more selected from a group consisting of glutaraldehyde, polyethyleneimine, poly-L-lysine, poly-D-lysine and polydopamine.

15 . The method according to claim 12 , which further comprises, after (2) and before (3), (2)′ maintaining the compressed state of the elastic substrate onto which the scaffold composition is loaded.

16 . The method according to claim 15 , wherein, in (2)′, the compressed state of the elastic substrate is maintained for 1-10 minutes.

17 . The method according to claim 15 , wherein, in (2)′, the scaffold composition is cured partially by maintaining the compressed state of the elastic substrate.

18 . The method according to claim 12 , wherein, in (1), the elastic substrate is compressed along a direction perpendicular to the groove.

19 . The method according to claim 12 , wherein, in (1), the elastic substrate is compressed by 5-80% of its original width before the compression along a direction perpendicular to the groove; and/or is compressed to close 30-100% of the width of the groove.

Assignees

• Korea Inst Sci & Tech

Inventors

• Choi Nakwon
• Kim Sohyun
• JEONG Sohyeon
• Yoon Eui Sung
• Lee Changjoon Justin
• Cho Il-Joo

Classifications

• C12N2533/54

  Collagen; Gelatin · CPC title

• B29K2995/0046

  Elastic · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C12M25/14

  Scaffolds; Matrices (in general C12N5/0068) · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title