Method and composition for the treatment, prevention, and diagnosis of cancer containing or derived from cancer stem cells

Patent metadata

Field	Value
Publication number	US-2017240902-A1
Application number	US-201715410480-A
Country	US
Kind code	A1
Filing date	Jan 19, 2017
Priority date	Aug 20, 2010
Publication date	Aug 24, 2017
Grant date	—

Abstract

The invention provides a method and composition for the treatment, prevention, and diagnosis of cancer containing or derived from cancer stem cells.

First claim

1 - 5 . (canceled)

6 . A method of treating or preventing a cancer containing or derived from cancer stem cells comprising a step of administering an siRNA-carrier complex comprising an siRNA against RPN2 and a transport carrier to a subject in need thereof.

7 . The method of claim 6 , wherein the transport carrier comprises the peptide AAAAAAK (SEQ ID NO: 26)

8 . The method of claim 6 , wherein the siRNA-carrier complex is administered locally.

9 . The method of claim 6 , wherein the siRNA-carrier complex is administered systemically.

10 . The method of claim 6 , wherein a double-stranded region of the siRNA is 19 bases in length and the siRNA inhibits the RPN2.

11 . The method of claim 6 , wherein a double-stranded region of the siRNA comprises position of 1,194 of the RPN2 gene sequence (SEQ ID NO: 1) and the siRNA inhibits the RPN2.

12 . The method of claim 6 , wherein a double-stranded region of the siRNA corresponds to at least position 1,212 of the RPN2 gene sequence (SEQ ID NO: 1) and the siRNA inhibits the RPN 2.

13 . The method of claim 6 , wherein the siRNA corresponds to positions comprises 1,194-1,212 of the RPN2 gene sequence (SEQ ID NO: 1) and the siRNA inhibits the RPN2.

14 . The method of claim 6 , wherein the siRNA comprises RNA comprises SEQ ID NO: 2 and the siRNA inhibits the RPN2.

15 . The method of claim 6 , wherein the siRNA comprises RBA comprising SEQ ID NO: 3 and the siRNA inhibits the RPN2.

16 . The method of claim 6 , further comprising administering a substance for introducing a nucleic acid.

17 . The method of claim 16 , wherein the substance for introducing a nucleic acid is liposome.

Assignees

• 3-D Matrix Ltd
• Nat Cancer Ct

Inventors

• Ochiya Takahiro

Classifications

• C12Q2600/106

  Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism · CPC title

• G01N2333/91097

  Hexosyltransferases (general) (2.4.1) · CPC title

• C12N2310/531

  Stem-loop; Hairpin · CPC title

• C12N2310/3513

  Protein; Peptide · CPC title

• C12Q1/6886

  for cancer (immunoassay for cancer G01N33/575) · CPC title