Method for producing hydrogel, method for enveloping envelopment target, and method for releasing envelopment target

Patent metadata

Field	Value
Publication number	US-2017239356-A1
Application number	US-201515304675-A
Country	US
Kind code	A1
Filing date	Apr 16, 2015
Priority date	Apr 17, 2014
Publication date	Aug 24, 2017
Grant date	—

Abstract

Provided is a method for producing a hydrogel, which enables a hydrogel comprising polyethylene glycol to be produced under low peroxidase concentration conditions and physiological conditions. The method for producing a hydrogel involves crosslinking polyethylene glycol having two or more thiol groups using peroxidase in the presence of a phenol compound.

First claim

1 . A method for producing a hydrogel, comprising crosslinking a polyethylene glycol having two or more thiol groups using a peroxidase in presence of a phenol compound.

2 . The method for producing a hydrogel according to claim 1 , wherein the peroxidase is a peroxidase derived from horseradish.

3 . The method for producing a hydrogel according to claim 1 , wherein
the phenol compound is at least one of tyramine hydrochloride, phenol, N-glycyl-L-tyrosine, hydroquinone, resorcinol, catechol and serotonin.

4 . The method for producing a hydrogel according to claim 1 , wherein
the polyethylene glycol having two or more thiol groups and a thiol compound having one or more thiol groups are crosslinked using the peroxidase in presence of the phenol compound.

5 . The method for producing a hydrogel according to claim 1 , wherein
the polyethylene glycol having two or more thiol groups and a phenol compound having one or more thiol groups are crosslinked using the peroxidase.

6 . A method for enveloping an envelopment target, comprising producing a hydrogel by crosslinking a polyethylene glycol having two or more thiol groups using a peroxidase in presence of a phenol compound, and enveloping the envelopment target in the hydrogel.

7 . A method for releasing an envelopment target, comprising releasing an envelopment target, which has been enveloped in a hydrogel by producing a hydrogel by crosslinking a polyethylene glycol having two or more thiol groups using a peroxidase in presence of a phenol compound and in presence of the envelopment target, by dissolving the hydrogel using a reducing agent.

Assignees

• Kamiya Noriho
• Moriyama Kosuke
• Hitachi Ltd

Inventors

• Kamiya Noriho
• Moriyama Kosuke
• Minamihata Kosuke

Classifications

• A61K47/10

  Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers · CPC title

• C12Y111/01007

  Peroxidase (1.11.1.7), i.e. horseradish-peroxidase · CPC title

• A61K9/06

  Ointments; Bases therefor; {Other semi-solid forms, e.g. creams, sticks, gels (composition of ointments, creams or gels A61K47/00)} · CPC title

• A61K47/42

  Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein (oligopeptides having up to five amino acids {A61K47/183}; polyamino acids A61K47/34) · CPC title

• A61K47/34 Primary

  Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers (A61K47/10 takes precedence) · CPC title