Method for priming hollow-fiber membrane module

Patent metadata

Field	Value
Publication number	US-2017232180-A1
Application number	US-201715581259-A
Country	US
Kind code	A1
Filing date	Apr 28, 2017
Priority date	Oct 31, 2014
Publication date	Aug 17, 2017
Grant date	—

Abstract

Provided is a priming method including a step of filling a priming liquid in a hollow-fiber membrane module in which hollow-fiber membranes are packed in a vessel having an inlet port, an outlet port, and a filtrate discharge port at a linear velocity of 20 cm/min or more and 550 cm/min or less through the inlet port or the outlet port in an amount of 15% or more relative to a volume of the hollow-fiber membrane module. According to the present invention, the effective filtration area at the time of cell suspension treatment is increased, and the recovery rate of cells and the filtration rate can be improved. Further, since the cell treatment can be completed while maintaining the closed environment, the obtained cells can be provided for therapeutic applications.

First claim

1 . A method for priming a hollow-fiber membrane module in which hollow-fiber membranes are packed in a vessel having an inlet port, an outlet port, and a filtrate discharge port, comprising: filling a priming liquid in the hollow-fiber membrane module in an amount of 15% or more relative to a volume of the hollow-fiber membrane module at a linear velocity of 20 cm/min or more and 550 cm/min or less from the inlet port or the outlet port of the hollow-fiber membrane module.

2 . The method according to claim 1 , wherein a pressure difference between internal pressure and external pressure of a hollow fiber membrane at the time of priming is 7 mmHg or more and 29 mmHg or less.

3 . The method according to claim 2 , wherein the internal pressure is negative pressure.

4 . The method according to claim 1 , wherein the linear velocity is 35 cm/min or more and 450 cm/min or less.

5 . The method according to claim 1 , wherein the priming liquid is filled in the hollow-fiber membrane module in an amount of 70% or more relative to a volume of the hollow-fiber membrane module.

6 . The method according to claim 1 , wherein the priming liquid is introduced into the inside of the hollow fiber membrane.

7 . The method according to claim 1 , wherein the inlet port and the outlet port communicate with each other through the inside of the hollow fiber membrane.

8 . The method according to claim 1 , wherein the hollow fiber membrane has pores with a pore diameter of 0.07 μm or more and 1.5 μm or less.

9 . The method according to claim 1 , wherein the priming liquid is at least one selected from the group consisting of physiological saline, infusion, serum, and a medium.

10 . A method for producing a cell concentrate, comprising:
after applying the method according to claim 1 to the hollow-fiber membrane module, introducing a cell suspension into the hollow-fiber membrane module from an inlet/outlet port of the hollow-fiber membrane module, and concentrating the cell suspension while discharging a filtrate containing no cells from the filtrate discharge port.

11 . The method according to claim 10 , wherein the hollow-fiber membrane module provided with the filtrate discharge port, the number of which is at least one, is used.

12 . The method according to claim 10 , wherein the cell suspension is introduced into the hollow-fiber membrane module from the inlet/outlet port of the hollow-fiber membrane module at a linear velocity of 500 cm/min or more and 2000 cm/min or less.

13 . The method according to claim 12 , wherein the cell suspension is derived from the hollow-fiber membrane module through the filtrate discharge port at a filtration rate of 1500 mL/m 2 /min or more and 3750 mL/m 2 /min or less.

14 . The method according to claim 1 , wherein the linear velocity is 35 cm/min or more and 300 cm/min or less.

Assignees

• Kaneka Corp

Inventors

• Umeda Nobuyoshi
• Taniguchi Shuhei

Classifications

• A61M1/3643 Primary

  Priming, rinsing before or after use · CPC title

• B01D65/00

  Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes · CPC title

• B01D67/0088

  Physical treatment with compounds, e.g. swelling, coating or impregnation · CPC title

• A61M1/34

  Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration · CPC title

• A61M1/0259

  Apparatus for treatment of blood or blood constituents not otherwise provided for (for agitating A61M1/025; for separating blood components present in distinct layers in a container A61M1/029) · CPC title