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Inhibitory rna for enhanced protein production in recombinant mammalian cells
US2017226550A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2017226550-A1 |
| Application number | US-201515501503-A |
| Country | US |
| Kind code | A1 |
| Filing date | Aug 4, 2015 |
| Priority date | Aug 7, 2014 |
| Publication date | Aug 10, 2017 |
| Grant date | — |
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- Title
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- Abstract
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Abstract
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The invention provides methods and compositions for improving production of exogenous polypeptides in large scale culture of mammalian host cells. The methods and compositions utilize RNA interference to inhibit expression of one or more specific host cell proteins. The present invention is based on the identification of specific mammalian genes and sequences therein that are useful as RNAi targets to enhance the production of exogenous proteins in mammalian cell culture.
First claim
Opening claim text (preview).
1 . A method of producing a polypeptide, which comprises (a) providing a recombinant mammalian host cell capable of expressing the polypeptide; (b) culturing the host cell under conditions suitable for (i) effecting expression of the polypeptide and (ii) inhibiting expression of at least one RNAi target gene, and (c) recovering the expressed polypeptide, wherein the RNAi target gene is a mouse gene selected from the group of mouse genes listed in the table immediately below or is an orthologue of the selected mouse gene. Gene Mouse Gene No. (Accession No) 1 NM_024479 2 NM_023697 3 NM_013523 4 NM_029840 5 NM_023168 6 NM_019980 7 NM_013847 8 NM_026871 9 NM_133355 10 NM_001003909 11 NM_025350 12 NM_025292 13 NM_013415 14 NM_001081414 15 NM_133500 16 NM_007958 17 NM_021509 18 NM_177941 19 NM_080510 20 NM_001033156 21 NM_001081377 22 NM_001014995 23 NM_023543 24 NM_026009 25 NM_133684 26 NM_021491 27 NM_026984 28 NM_023131 29 NM_025650 30 NM_011217 31 NM_008048 32 NM_025865 33 NM_013855 34 NM_025964 35 NM_001004761 36 NM_019828 37 NM_011521 38 NM_008737 39 NM_008770 40 NM_001024385 41 NM_024267 42 NM_008246 43 NM_024454 44 NM_008626 45 NM_007647 46 NM_011169 47 NM_025485 48 NM_199314 49 NM_008206 50 FJ042496 51 NM_028052 52 NM_024198 53 NM_027880 54 NM_028004 55 NM_019688 56 NM_028006 57 NM_027880 58 NM_133895 59 NM_013563 60 NM_013632 61 NM_019724 62 NM_013659 63 NM_177909 64 NM_175638 65 NM_001081309 66 NM_011101 67 NM_029572 68 NM_183294 69 NM_199029 70 NM_023517 71 NM_024236 72 NM_012048 73 NM_020603 74 NM_008957 75 NM_138952 76 NM_001001321 77 NM_010588 78 NM_172844 79 NM_028450 80 NM_030037 81 NM_028475 82 NM_019773 83 NM_178076 84 NM_028784 85 NM_018852 86 NM_007979 87 NM_001037755 88 NM_173388 89 NM_133216 90 NM_029947 91 NM_001195413 92 NM_001110327 93 NM_019791 94 NM_028175 95 NM_028950 96 NM_021426 97 NM_033524 98 NM_013815 99 NM_028105
Assignees
Inventors
Classifications
- C12P21/02Primary
having a known sequence of two or more amino acids, e.g. glutathione · CPC title
- C12P21/00Primary
Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title
Stem-loop; Hairpin · CPC title
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title
interfering nucleic acids [NA] · CPC title
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- What does patent US2017226550A1 cover?
- The invention provides methods and compositions for improving production of exogenous polypeptides in large scale culture of mammalian host cells. The methods and compositions utilize RNA interference to inhibit expression of one or more specific host cell proteins. The present invention is based on the identification of specific mammalian genes and sequences therein that are useful as RNAi tar…
- Who is the assignee on this patent?
- Ye Jianxin, Alvin Krista, Zhang Siyan, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12P21/02. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Aug 10 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).