Proteins used for the diagnosis of lyme borreliosis

Patent metadata

Field	Value
Publication number	US-2017205407-A1
Application number	US-201615019520-A
Country	US
Kind code	A1
Filing date	Feb 9, 2016
Priority date	Aug 28, 2009
Publication date	Jul 20, 2017
Grant date	—

Abstract

Chimera proteins including: (i) at least one sequence of a DbpA protein of a Borrelia species selected from B. afzelii, B. burgdorferi sensu stricto and B. garinii , and (ii) at least one sequence of an OspC protein of a Borrelia species selected from B. afzelii, B. burgdorferi sensu stricto and B. garinii . Also, a method and a kit for the in vitro diagnosis of Lyme borreliosis using said proteins.

First claim

1 . A polypeptide comprising (i) a Decorin-binding protein A (DbpA) extracellular domain portion from B. afzelii, B. burgdorferi sensu stricto , or B. garinii that includes DbpA immunodominant epitope regions, and (ii) an Outer-surface protein C (OspC) extracellular domain portion from B. afzelii, B. burgdorferi sensu stricto , or B. garinii that includes OspC immunodominant epitope regions.

2 . The polypeptide as claimed in claim 1 , wherein the DbpA extracellular domain portion is on the N-terminal side of the polypeptide, and the OspC extracellular domain portion is on the C-terminal side of the polypeptide.

3 . The polypeptide as claimed in claim 1 , further comprising a linking region between the DbpA extracellular domain portion and the OspC extracellular domain portion.

4 . A polypeptide comprising (i) a first amino acid sequence having at least 40% sequence identity with any of the amino acid sequences selected from the group consisting of SEQ ID NOs: 1, 3, 5, and 6, and (ii) a second amino acid sequence having at least 50% sequence identity with any of the amino acid sequences selected from the group consisting of SEQ ID NOs: 2, 4, and 7.

5 . The polypeptide as claimed in claim 4 , wherein the first amino acid sequence is on the N-terminal side of the polypeptide, and the second amino acid sequence is on the C-terminal side of the polypeptide.

6 . The polypeptide as claimed in claim 4 , further comprising a linking region between the first amino acid sequence and the second amino acid sequence.

7 . The polypeptide as claimed in claim 4 , further comprising a third amino acid sequence of at least 6 contiguous histidines at the N-terminal or C-terminal end.

8 . The polypeptide as claimed in claim 4 , wherein the first amino acid sequence comprises any of the amino acid sequences of SEQ ID NOs: 1, 3, 5, or 6, and the second amino acid sequence comprises any of the amino acid sequences of SEQ ID NOs: 2, 4, or 7.

9 . The polypeptide as claimed in claim 4 , comprising the amino acid sequences of SEQ ID NO: 1 and SEQ ID NO: 2.

10 . The polypeptide as claimed in claim 4 , comprising the amino acid sequences of SEQ ID NO: 3 and SEQ ID NO: 4.

11 . The polypeptide as claimed in claim 4 , comprising the amino acid sequences of SEQ ID NO: 5 and SEQ ID NO: 7.

12 . The polypeptide as claimed in claim 4 , comprising the amino acid sequences of SEQ ID NO: 6 and SEQ ID NO: 7.

13 . The polypeptide as claimed in claim 4 , comprising the amino acid sequences of SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7.

14 . A nucleic acid encoding the polypeptide as claimed in claim 4 .

15 . A diagnostic kit comprising the polypeptide as claimed in claim 1 .

16 . The diagnostic kit as claimed in claim 15 , further comprising an anti-human-immunoglobulin labeled with a label.

17 . The diagnostic kit as claimed in claim 15 , further comprising a chimeric VlsE polypeptide.

18 . A method for the in vitro diagnosis of Lyme borreliosis using a biological sample, comprising:
bringing the biological sample into contact with at least one polypeptide as claimed in claim 1 ; and determining whether there is formation of an immunological complex between the polypeptide and antibodies of the biological sample.

19 . The method as claimed in claim 18 , wherein formation of the immunological complex is determined by adding at least one anti-human-immunoglobulin labeled with a label.

20 . The method as claimed in claim 18 , wherein the polypeptide is immobilized on a solid support.

Assignees

• Biomerieux Sa

Inventors

• Levet Lionel
• Mejan-Letourneur Odile

Classifications

• A61P37/04

  Immunostimulants · CPC title

• G01N2469/20

  Detection of antibodies in sample from host which are directed against antigens from microorganisms · CPC title

• G01N33/56911 Primary

  Bacteria · CPC title

• C07K14/195

  from bacteria · CPC title

• G01N2333/20

  from Spirochaetales (O), e.g. Treponema, Leptospira · CPC title