Light Microscope and Microscopy Method

Patent metadata

Field	Value
Publication number	US-2017192216-A1
Application number	US-201715442937-A
Country	US
Kind code	A1
Filing date	Feb 27, 2017
Priority date	Jan 25, 2013
Publication date	Jul 6, 2017
Grant date	—

Abstract

An optical microscope having a sample plane for positioning a sample, and a light source for emitting illumination light, includes optical imaging means for guiding the illumination light into the sample plane. A detector device having a plurality of detector elements for detecting sample light coming from the sample. Adjacent detector elements are at a distance from one another which is smaller than an Airy-Disk produced by a point of the sample plane on the detector device. A scanning device has at least a first and a second optical arrangement simultaneously movable in a common direction for producing an illumination scanning movement and a detection scanning movement, which are opposite to one another. Sample regions spaced apart from one another can be examined simultaneously, such that both a beam path of the sample light from the sample plane to the detector device and a beam path of the illumination light from the light source to the sample plane run via the first optical arrangement and only one of these two beam paths runs via the second optical arrangement.

First claim

What is claimed is:

1 . An optical microscope comprising a sample plane in which a sample to be examined is positionable, a light source for emitting illumination light, optical imaging means for guiding the illumination light into the sample plane, and a detector device having a plurality of detector elements for the purpose of detecting sample light coming from the sample, wherein adjacent detector elements are at a distance from one another which is smaller than an Airy disk produced by a point of the sample plane on the detector device, a scanning device having at least one first and one second optical arrangements, said optical arrangements being simultaneously movable in a common direction for the purpose of producing an illumination scanning movement and a detection scanning movement, said illumination and said detection scanning movements being opposite to one another, wherein the illumination scanning movement is a scanning movement of illumination light over the sample plane, and wherein as detection scanning movement receiving regions of the detector elements are movable over the sample plane, said first and second optical arrangements each have a plurality of optical elements which are arranged alongside one another and by means of which sample regions spaced apart from one another are simultaneously examinable, said first and second optical arrangements being arranged such that both a beam path of the sample light from the sample plane to the detector device and a beam path of the illumination light from the light source to the sample plane run via the first optical arrangement and only one of these two beam paths runs via the second optical arrangement.

2 . The optical microscope as claimed in claim 1 , wherein the imaging scale is 1:2.

3 . The optical microscope as claimed in claim 1 wherein for the purpose of the non-inverted imaging, the first or second optical arrangements each have a light-converging effect and the optical elements of the other optical arrangement each have a light-diverging effect for the purpose of the non-inverted imaging.

4 . The optical microscope as claimed in claim 1 wherein the scanning device has a third optical arrangement, which comprises one or a plurality of image field rotators and is arranged in the beam path of the sample light and wherein, inverted imagings are generable by means of the first optical arrangement or by means of the first and second optical arrangements, which inverted imagings are convertible into non-inverted imagings by the image field rotation of the image field rotator or rotators.

5 . The optical microscope as claimed in claim 4 , wherein the scanning device has a third and a fourth optical arrangement as image field rotators, said optical arrangements each having an optical element for each of the optical elements of the first optical arrangement.

6 . The optical microscope as claimed in claim 4 , wherein the beam path of the sample light from the sample plane to the detector device runs via the first and second optical arrangements, and wherein the optical elements of the first and second optical arrangements each have a light-converging effect.

7 . The optical microscope as claimed in claim 1 , further comprising a pinhole stop arrangement, which is movable jointly with the first and second optical arrangements for generating a confocal sample image between the first optical arrangement and the sample plane, said pinhole stop arrangement having a respective pinhole stop for each optical element of the first optical arrangement.

8 . The optical microscope as claimed in claim 1 , wherein the optical arrangements of the scanning device are embodied in each case by a rotatable disk.

9 . The optical microscope as claimed in claim 8 , wherein said rotable disks are mounted on a common driveshaft.

10 . The optical microscope as claimed in claim 1 , further comprising an actuating device for linearly displacing the optical arrangements of the scanning device.

11 . The optical microscope as claimed in claim 1 , wherein said optical elements of the first optical arrangement are formed in each case by at least one lens, a mirror or a light-diffracting element, and wherein said optical elements of the second optical arrangement are formed in each case by at least one lens, a mirror or a light-diffracting element.

12 . The optical microscope as claimed in claim 1 wherein said scanning device has a zoom optical arrangement which is movable jointly with the first optical arrangement and which is arranged such that the sample light solely passes through it during operation.

13 . The optical microscope as claimed in claim 1 , further comprising a mirror or prism provided as a retroreflector in the illumination beam path, wherein the illumination light passes through the retroreflector in the direction of micromirrors which have a pinhole stop for passing a portion of the illumination light in the direction of the sample.

14 . A microscopy method for examining a sample positioned in a sample plane of an optical microscope, comprising guiding illumination light into the sample plane moving the illumination light as illumination scanning movement over the sample plane, detecting sample light coming from the sample by means of a detector device having a plurality of detector elements, wherein adjacent detector elements are at a distance from one another which is smaller than an Airy disk produced by a point of the sample plane on the detector device simultaneously moving optical arrangements of a scanning device in a common direction for producing said illumination scanning movement and a detection scanning movement, said illumination scanning and detection scanning movements being opposite to one another, wherein as detection scanning movement receiving regions of the detector elements are moved over the sample plane, said sample regions being spaced apart from one another are examined simultaneously by means of the first and second optical arrangements, each having a plurality of optical elements arranged alongside one another, said first and second optical arrangements being arranged such that illumination light and sample light are guided via the first optical arrangement, said second optical arrangement being arranged so that only either illumination light is guided toward the sample plane or sample light is guided toward the detector device.

15 . The microscopy method as claimed in claim 14 , further comprising carrying out the illumination scanning movement and the detection scanning movement during an integration time of detector elements of the detector device.

Assignees

• Zeiss Carl Microscopy Gmbh

Inventors

• Bathe Wolfgang
• Netz Ralf

Classifications

• G02B21/0044

  moving apertures, e.g. Nipkow disks, rotating lens arrays · CPC title

• G02B21/0032 Primary

  Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06) · CPC title

• G02B21/025

  with variable magnification (variable magnification G02B15/00) · CPC title

• G02B21/0072 Primary

  details concerning resolution or correction, including general design of CSOM objectives · CPC title

• G02B21/008

  Details of detection or image processing, including general computer control · CPC title