Method for evaluating condition of skin dryness

Patent metadata

Field	Value
Publication number	US-2017192015-A1
Application number	US-201515308724-A
Country	US
Kind code	A1
Filing date	May 8, 2015
Priority date	May 8, 2014
Publication date	Jul 6, 2017
Grant date	—

Abstract

To provide a method for quickly and accurately evaluating a condition of skin dryness and a method for efficiently searching a substance to improve dry skin. A method for evaluating a condition of skin dryness, the method comprising measuring the expression levels of AGR2 and/or AGR3 in skin cells collected from subjects.

First claim

1 : A method for evaluating a condition of skin dryness, the method comprising measuring the expression levels of AGR2 and/or AGR3 in skin cells collected from a subject.

2 : The method according to claim 1 , wherein the condition of skin dryness is a condition of rough skin caused by skin dryness.

3 : The method according to claim 2 , wherein the rough skin caused by skin dryness is selected from the group consisting of dry skin, sensitive skin, xeroderma, senile xerosis, ichthyosis and dry eczema.

4 : A method for evaluating or selecting an agent for ameliorating dry skin, the method comprising the following (A) to (D):
(A) administering a test substance to a subject or a subject animal; (B) measuring the expression levels of AGR2 and/or AGR3 in skin cells collected from the subject or subject animal; (C) comparing the expression levels measured in the (B) with the expression levels of AGR2 and/or AGR3 in skin cells collected from a subject or a subject animal to which the test substance is not administered; and (D) evaluating the effect of the test substance to increase the expression levels of AGR2 and/or AGR3 based on the result of the (C).

5 : A method for evaluating or selecting an agent for ameliorating dry skin, the method comprising the following (A′) to (D′):
(A′) contacting a test substance with a tissue or cells which are derived from a mammal and which can express AGR2 and/or AGR3;
(B′) measuring the expression levels of AGR2 and/or AGR3 in the tissue or cells;
(C′) comparing the expression levels measured in the (B′) with the expression levels of AGR2 and/or AGR3 in a control group; and
(D′) evaluating the effect of the test substance to increase the expression levels of AGR2 and/or AGR3 based on the result of the (C′).

6 : The method according to claim 4 , further comprising the following:
(E) selecting the test substance that increases the expression levels of AGR2 and/or AGR3 in the (D) as an agent for ameliorating dry skin based on the evaluation in the (D).

7 : The method according to claim 5 , further comprising the following:
(E′) selecting the test substance that increases the expression levels of AGR2 and/or AGR3 in the (D′) as an agent for ameliorating dry skin based on the evaluation in the (D′).

8 : The method according to claim 4 , wherein the agent for ameliorating dry skin is an agent for ameliorating rough skin caused by skin dryness.

9 : The method according to claim 8 , wherein the rough skin caused by skin dryness is selected from the group consisting of dry skin, sensitive skin, xeroderma, senile xerosis, ichthyosis and dry eczema.

10 : The method according to claim 5 , wherein the agent for ameliorating dry skin is an agent for ameliorating rough skin caused by skin dryness.

11 : The method according to claim 10 , wherein the rough skin caused by skin dryness is selected from the group consisting of dry skin, sensitive skin, xeroderma, senile xerosis, ichthyosis and dry eczema.

12 : The method according to claim 6 , wherein the agent for ameliorating dry skin is an agent for ameliorating rough skin caused by skin dryness.

13 : The method according to claim 12 , wherein the rough skin caused by skin dryness is selected from the group consisting of dry skin, sensitive skin, xeroderma, senile xerosis, ichthyosis and dry eczema.

14 : The method according to claim 7 , wherein the agent for ameliorating dry skin is an agent for ameliorating rough skin caused by skin dryness.

15 : The method according to claim 14 , wherein the rough skin caused by skin dryness is selected from the group consisting of dry skin, sensitive skin, xeroderma, senile xerosis, ichthyosis and dry eczema.

Assignees

• Kao Corp

Inventors

• Ito Shotaro
• Ishikawa Junko

Classifications

• G01N33/6893 Primary

  related to diseases not provided for elsewhere · CPC title

• C12Q1/533 Primary

  involving isomerase · CPC title

• G01N2800/20

  Dermatological disorders · CPC title

• G01N2500/00

  Screening for compounds of potential therapeutic value · CPC title

• G01N33/50

  Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing (measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q) · CPC title