Reusable chemical or biological sensor and method for using same

Patent metadata

Field	Value
Publication number	US-2017191991-A1
Application number	US-201515313337-A
Country	US
Kind code	A1
Filing date	May 29, 2015
Priority date	May 29, 2014
Publication date	Jul 6, 2017
Grant date	—

Abstract

For a chemical or biological sensor, which is reusable while maintaining a clean state thereof, and a method for using the same, the present invention provides a reusable chemical or biological sensor and a method for using the same, the reusable chemical or biological sensor comprising: a sensor transducer; a ferromagnetic pattern formed on at least one surface of the sensor transducer; magnetic nanoparticles which can be collected or released in a single layer on the sensor transducer, in directions of first and second magnetic fields applied to the sensor transducer; and a receptor which is fixed on the magnetic nanoparticles and can bind to a target substance to be detected.

First claim

1 . A reusable chemical or biological sensor comprising:
a sensor transducer; a ferromagnetic pattern formed on at least one surface of the sensor transducer; a magnetic nanoparticle that can be collected in a single layer on the sensor transducer or released from the sensor transducer, depending on the directions of a first magnetic field and a second magnetic field that are applied to the sensor transducer; and a receptor that is fixed to the magnetic nanoparticle and can be bound to a target substance that is to be detected.

2 . The sensor of claim 1 ,
wherein the ferromagnetic pattern is a pattern that includes at least one of nickel and gold, and wherein the sensor transducer includes a carbon nanotube-based sensor transducer formed on a substrate that includes at least one of silicon and silicon oxide.

3 . The sensor of claim 2 ,
wherein the ferromagnetic pattern includes a polyethylene glycol (PEG) passivation layer, and the sensor transducer includes an octadecyltrichlorosilane (OTS) passivation layer.

4 . The sensor of claim 2 ,
wherein the receptor is an antibody and the target substance is an antigen.

5 . The sensor of claim 1 ,
wherein the first magnetic field and the second magnetic field are applied in directions opposite to each other to the sensor transducer.

6 . The sensor of claim 5 ,
wherein the receptor can be collected on the sensor transducer due to differences in magnetic intensity of the first magnetic field, which is caused by the ferromagnetic pattern.

7 . The sensor of claim 5 ,
wherein the receptor can be released from the sensor transducer due to differences in magnetic intensity of the second magnetic field, which is caused by the ferromagnetic pattern.

8 . A method for using a reusable chemical or biological sensor, the method performing at least once a unit cycle that comprises:
preparing the chemical or biological sensor of claim 1 ; collecting the magnetic nanoparticle and the receptor that is fixed to the magnetic nanoparticle on the ferromagnetic pattern by applying a first magnetic field to the chemical or biological sensor; receiving a target substance in the receptor by providing the target substance to the chemical or biological sensor; detecting the target substance by the chemical or biological sensor using an optical method or an electrical signal measurement method; and releasing the magnetic nanoparticle and the receptor by applying a second magnetic field which is in a direction opposite to the first magnetic field to the chemical or biological sensor.

9 . A method for using a reusable chemical or biological sensor, the method performing at least once a unit cycle that comprises:
preparing the chemical or biological sensor of claim 2 ; collecting the magnetic nanoparticle and the receptor that is fixed to the magnetic nanoparticle on the ferromagnetic pattern by applying a first magnetic field to the chemical or biological sensor; receiving a target substance in the receptor by providing the target substance to the chemical or biological sensor; detecting the target substance by the chemical or biological sensor using an optical method or an electrical signal measurement method; and releasing the magnetic nanoparticle and the receptor by applying a second magnetic field which is in a direction opposite to the first magnetic field to the chemical or biological sensor.

10 . A method for using a reusable chemical or biological sensor, the method performing at least once a unit cycle that comprises:
preparing the chemical or biological sensor of claim 3 ; collecting the magnetic nanoparticle and the receptor that is fixed to the magnetic nanoparticle on the ferromagnetic pattern by applying a first magnetic field to the chemical or biological sensor; receiving a target substance in the receptor by providing the target substance to the chemical or biological sensor; detecting the target substance by the chemical or biological sensor using an optical method or an electrical signal measurement method; and releasing the magnetic nanoparticle and the receptor by applying a second magnetic field which is in a direction opposite to the first magnetic field to the chemical or biological sensor.

11 . A method for using a reusable chemical or biological sensor, the method performing at least once a unit cycle that comprises:
preparing the chemical or biological sensor of claim 4 ; collecting the magnetic nanoparticle and the receptor that is fixed to the magnetic nanoparticle on the ferromagnetic pattern by applying a first magnetic field to the chemical or biological sensor; receiving a target substance in the receptor by providing the target substance to the chemical or biological sensor; detecting the target substance by the chemical or biological sensor using an optical method or an electrical signal measurement method; and releasing the magnetic nanoparticle and the receptor by applying a second magnetic field which is in a direction opposite to the first magnetic field to the chemical or biological sensor.

12 . A method for using a reusable chemical or biological sensor, the method performing at least once a unit cycle that comprises:
preparing the chemical or biological sensor of claim 5 ; collecting the magnetic nanoparticle and the receptor that is fixed to the magnetic nanoparticle on the ferromagnetic pattern by applying a first magnetic field to the chemical or biological sensor; receiving a target substance in the receptor by providing the target substance to the chemical or biological sensor; detecting the target substance by the chemical or biological sensor using an optical method or an electrical signal measurement method; and releasing the magnetic nanoparticle and the receptor by applying a second magnetic field which is in a direction opposite to the first magnetic field to the chemical or biological sensor.

13 . A method for using a reusable chemical or biological sensor, the method performing at least once a unit cycle that comprises:
preparing the chemical or biological sensor of claim 6 ; collecting the magnetic nanoparticle and the receptor that is fixed to the magnetic nanoparticle on the ferromagnetic pattern by applying a first magnetic field to the chemical or biological sensor; receiving a target substance in the receptor by providing the target substance to the chemical or biological sensor; detecting the target substance by the chemical or biological sensor using an optical method or an electrical signal measurement method; and releasing the magnetic nanoparticle and the receptor by applying a second magnetic field which is in a direction opposite to the first magnetic field to the chemical or biological sensor.

14 . A method for using a reusable chemical or biological sensor, the method performing at least once a unit cycle that comprises:
preparing the chemical or biological sensor of claim 7 ; collecting the magnetic nanoparticle and the receptor that is fixed to the magnetic nanoparticle on the ferromagnetic pattern by applying a first magnetic field to the chemical or biological sensor; receiving a target substance in the receptor by providing the target substance to the chemical or biological sensor; detecting the target substance by the chemical or biological sensor using an optical method or an electrical signal measurement method; and releasing the magnetic nanoparticle and the receptor by applying a second magnetic field which is in a direction opposite to the first magnetic field to the chemical or biological sensor.

Assignees

• Univ Seoul Nat R & Db Found

Inventors

• Hong Seung Hun
• Chen Xing
• Yoo Ha Neul

Classifications

• G01N33/551

  the carrier being inorganic · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• G01N33/5302 Primary

  Apparatus specially adapted for immunological test procedures · CPC title

• G01N21/645

  Specially adapted constructive features of fluorimeters · CPC title

• G01N2446/00

  Magnetic particle immunoreagent carriers · CPC title