Method of synthesizing biogenic elemental selenium nanostructure using enterobacter cloacae and application thereof

Patent metadata

Field	Value
Publication number	US-2017191083-A1
Application number	US-201715397711-A
Country	US
Kind code	A1
Filing date	Jan 3, 2017
Priority date	Jan 4, 2016
Publication date	Jul 6, 2017
Grant date	—

Abstract

A method of synthesizing biogenic elemental selenium nanostructure using Enterobacter cloacae and its application. The method uses E. cloacae Z0206 to reduce selenite to zero valence selenium and forms nano-sized elemental selenium particles, including steps of inoculating activated E. cloacae Z0206 to fermentation broth, adding sodium selenite solution, shaking and incubating, collecting the fermentation broth and separating the elemental selenium nanoparticles.

First claim

What is claimed is:

1 . A method of synthesizing biogenic elemental selenium nanoparticles using selenite as starting materials and E. cloacae Z0206 as fermentative bacteria, comprising the steps of:
inoculating activated E. cloacae Z0206 cells to a fermentation broth, and adding sodium selenite solution to obtain an inoculated broth; shaking and incubating the inoculated broth to obtain a fermented broth; and collecting the fermented broth, performing a first centrifugation on the fermented broth to obtain a supernatant, performing a second centrifugation on the supernatant to obtain a sediment, re-suspending the sediment with double distilled water to obtain a suspension, ultrasonicating the suspension, adding hexane, and mixing and stratifying by standing, wherein parameters of the first centrifugation are 4° C., 5,000×g, 15 minutes, parameters of the second centrifugation are 4° C., 25,000×g, 15 minutes, parameters of the ultrasonication are 25 W, 5 seconds on and 5 seconds off for 15 minutes, and the volume of the hexane is half of the volume of the suspension.

2 . The method of claim 1 , wherein the fermentation broth consists of sucrose (25 g·L −1 ), yeast extraction (5 g·L −1 ), tryptone (5 g·L −1 ), K 2 HPO 4 ·3H 2 O (2.62 g·L −1 ), KH 2 PO 4 (1 g·L −1 ) and MgSO 4 (0.5 g·L −1 ), with an initial pH value of 7.5.

3 . The method of claim 1 , wherein the final concentration of sodium selenite in the fermentation broth is 10 mM.

4 . The method of claim 1 , wherein the temperature, rotation speed and incubation time are 32° C., 250 rpm and 144 hours, respectively.

Assignees

• Univ Zhejiang

Inventors

• Wang Yizhen
• Song Deguang
• Lu Zeqing
• Wang Fengqin
• Cheng Yuanzhi
• Li Xiaoxiao

Classifications

• C12P3/00 Primary

  Preparation of elements or inorganic compounds except carbon dioxide {(recovery of carbon dioxides as by-products C12F3/02)} · CPC title

• A23K20/00

  Accessory food factors for animal feeding-stuffs · CPC title

• A23K20/20

  Inorganic substances, e.g. oligoelements · CPC title

• A23K10/18

  of live microorganisms · CPC title

• A23K50/30

  for swines · CPC title