HERBICIDE TOLERANT COTTON EVENT pDAB4468.19.10.3

Patent metadata

Field	Value
Publication number	US-2017191076-A1
Application number	US-201715452422-A
Country	US
Kind code	A1
Filing date	Mar 7, 2017
Priority date	Jan 23, 2012
Publication date	Jul 6, 2017
Grant date	—

Abstract

Cotton event pDAB4468.19.10.3 comprises genes encoding AAD-12 and PAT, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection.

First claim

What is claimed is:

1 . A transgenic cotton seed comprising in its genome a pat/aad-12 transgenic event consisting of a polynucleotide that is at least 95% identical to SEQ ID NO:21.

2 . The transgenic cotton seed of claim 1 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1329-1380 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 143-194 of SEQ ID NO:2.

3 . The transgenic cotton seed of claim 2 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1254-1455 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 118-219 of SEQ ID NO:2

4 . The transgenic cotton seed of claim 3 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1304-1405 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 68-269 of SEQ ID NO:2

5 . The transgenic cotton seed of claim 4 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1054-1655 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 1-369 of SEQ ID NO:2

6 . The transgenic cotton seed of claim 1 , wherein the polynucleotide is at least 98% identical to SEQ ID NO:21.

7 . The transgenic cotton seed of claim 1 , wherein the polynucleotide is SEQ ID NO:21.

8 . A transgenic cotton plant comprising in its genome a pat/aad-12 transgenic event consisting of a polynucleotide that is at least 95% identical to SEQ ID NO:21.

9 . A part of the transgenic cotton plant of claim 8 , wherein the part is selected from the group consisting of pollen, ovule, flowers, bolls, shoots, roots, and leaves.

10 . A method for breeding a cotton plant, the method comprising:
crossing the transgenic cotton plant of claim 8 with a second cotton plant to produce a progeny cotton plant; and assaying the progeny cotton plant for the presence of the pat/aad-12 transgenic event.

11 . The method according to claim 10 , wherein assaying the progeny cotton plant for the presence of the pat/aad-12 transgenic event comprises amplifying genomic DNA from the progeny plant in a polymerase chain reaction or applying an herbicide to the progeny cotton plant, wherein the herbicide is a phenoxyacetic acid herbicide, a pyridoxyacetic acid herbicide, or glufosinate.

12 . A method for identifying a cotton product from a genetically-modified organism, the method comprising:
isolating nucleic acid molecules from a cotton meal, cotton fiber, or cotton oil; and screening the nucleic acid molecules for a pat/aad-12 transgenic event consisting of a polynucleotide that is at least 95% identical to SEQ ID NO:21.

13 . The method according to claim 12 , wherein screening the nucleic acid molecules comprises contacting the molecules with an oligonucleotide probe that hybridizes to SEQ ID NO:1 or SEQ ID NO:2 under highly stringent conditions, but does not hybridize under highly stringent conditions to a polynucleotide consisting of a first 5′ contiguous nucleotide sequence of nucleotides 1-1354 of SEQ ID NO:1 and a second 3′ contiguous nucleotide sequence of nucleotides 169-2898 of SEQ ID NO:2.

14 . A cotton meal, cotton fiber, or cotton oil from a genetically-modified plant, the meal, fiber, or oil comprising a polynucleotide that is at least 95% identical to SEQ ID NO:21.

15 . A method for controlling weeds in a cotton crop comprising the transgenic cotton plant of claim 8 , the method comprising:
applying an herbicide to the cotton crop, wherein the herbicide is a phenoxyacetic acid herbicide, a pyridoxyacetic acid herbicide, or glufosinate.

16 . The method according to claim 15 , wherein the herbicide is a phenoxyacetic acid herbicide.

17 . The method according to claim 16 , wherein the phenoxyacetic acid herbicide is 2,4-D or MCPA.

18 . The method according to claim 15 , wherein the herbicide is a pyridoxyacetic acid herbicide.

19 . The method according to claim 18 , wherein the pyridoxyacetic acid herbicide is triclopyr or fluroxypyr.

20 . The method according to claim 15 , wherein the herbicide is glufosinate.

Assignees

• Dow Agrosciences Llc

Inventors

• Cui Yunxing C
• King Raina
• Kaiser Tina M
• Robinson Andrew E
• Pareddy Dayakar
• Toledo Sandra G
• Braxton Leon B
• Anderson David M
• Wright Terry R

Classifications

• C12N15/8277

  Phosphinotricin · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12Q2600/16

  Primer sets for multiplex assays · CPC title

• A01N57/20

  containing acyclic or cycloaliphatic radicals · CPC title

• C12Q1/6876

  Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title