Passage timing calculation device, passage timing calculation method, and recording medium for recording program
US-2024352397-A1 · Oct 24, 2024 · US
US2017191037A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2017191037-A1 |
| Application number | US-201615224499-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jul 29, 2016 |
| Priority date | Dec 30, 2015 |
| Publication date | Jul 6, 2017 |
| Grant date | — |
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Provided are a method of culturing an induced pluripotent stem cell and a method of forming a spheroid of the induced pluripotent stem cell. According to an embodiment of the present inventive concept, the induced pluripotent stem cell may have improved stemness.
Opening claim text (preview).
What is claimed is: 1 . A container for culturing an induced pluripotent stem cell, the container having a nanostructure array. 2 . The container of claim 1 , wherein the nanostructure array has a plurality of nanocolumns that are spaced at random intervals on a substrate. 3 . The container of claim 2 , wherein the length of each nanocolumn is in a range of about 0.5 μm to about 1.5 μm. 4 . The container of claim 2 , wherein the density of nanocolumns in the nanostructure array is in range of about 150/100 μm 2 to about 1,000/100 μm 2 (the number of nanocolumns/area of nanostructure array). 5 . The container of claim 1 , wherein the container is designed to culture the induced pluripotent stem cell in an undifferentiated condition under a feeder layer-free. 6 . The container of claim 1 , wherein the container is for forming a spheroid of the induced pluripotent stem cell. 7 . The container of claim 1 , wherein the nanostructure array is reusable. 8 . A method of culturing an induced pluripotent stem cell under an undifferentiated condition, the method comprising: culturing an induced pluripotent stem cell in a nanostructure array. 9 . The method of claim 8 , wherein the nanostructure array has a plurality of nanocolumns that are spaced at random intervals on a substrate. 10 . The method of claim 8 , wherein the length of each nanocolumn in the nanostructure array is in a range of about 0.5 μm to about 1.5 μm. 11 . The method of claim 8 , wherein the density of nanocolumns in the nanostructure array is in range of about 150/100 μm 2 to about 1,000/100 μm 2 (the number of nanocolumns/area of nanostructure array). 12 . A method of forming a spheroid of an induced pluripotent stem cell, the method comprising: culturing an induced pluripotent stem cell on a nanostructure array. 13 . The method of claim 12 , wherein the nanostructure array has a plurality of nanocolumns that are spaced at random intervals on a substrate. 14 . The method of claim 12 , wherein the length of each nanocolumn in the nanostructure array is in a range of about 0.5 μm to about 1.5 μm. 15 . The method of claim 12 , the density of nanocolumns in the nanostructure array is in range of about 150/100 μm 2 to about 1,000/100 μm 2 (the number of nanocolumns/area of nanostructure array).
Microcarriers · CPC title
Supports or coatings for cell culture characterised by topography · CPC title
using microcarriers · CPC title
Artificially induced pluripotent stem cells, e.g. iPS · CPC title
Transcription factors · CPC title
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