Method and container for culturing undifferentiated induced pluripotent stem cell

US2017191037A1 · US · A1

Patent metadata
FieldValue
Publication numberUS-2017191037-A1
Application numberUS-201615224499-A
CountryUS
Kind codeA1
Filing dateJul 29, 2016
Priority dateDec 30, 2015
Publication dateJul 6, 2017
Grant date

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  1. Title

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  2. Abstract

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  3. Assignees and inventors

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  4. Key dates

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  5. First independent claim

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  6. CPC / IPC classifications

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  7. Citations and related patents

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Abstract

Official abstract text for this publication.

Provided are a method of culturing an induced pluripotent stem cell and a method of forming a spheroid of the induced pluripotent stem cell. According to an embodiment of the present inventive concept, the induced pluripotent stem cell may have improved stemness.

First claim

Opening claim text (preview).

What is claimed is: 1 . A container for culturing an induced pluripotent stem cell, the container having a nanostructure array. 2 . The container of claim 1 , wherein the nanostructure array has a plurality of nanocolumns that are spaced at random intervals on a substrate. 3 . The container of claim 2 , wherein the length of each nanocolumn is in a range of about 0.5 μm to about 1.5 μm. 4 . The container of claim 2 , wherein the density of nanocolumns in the nanostructure array is in range of about 150/100 μm 2 to about 1,000/100 μm 2 (the number of nanocolumns/area of nanostructure array). 5 . The container of claim 1 , wherein the container is designed to culture the induced pluripotent stem cell in an undifferentiated condition under a feeder layer-free. 6 . The container of claim 1 , wherein the container is for forming a spheroid of the induced pluripotent stem cell. 7 . The container of claim 1 , wherein the nanostructure array is reusable. 8 . A method of culturing an induced pluripotent stem cell under an undifferentiated condition, the method comprising: culturing an induced pluripotent stem cell in a nanostructure array. 9 . The method of claim 8 , wherein the nanostructure array has a plurality of nanocolumns that are spaced at random intervals on a substrate. 10 . The method of claim 8 , wherein the length of each nanocolumn in the nanostructure array is in a range of about 0.5 μm to about 1.5 μm. 11 . The method of claim 8 , wherein the density of nanocolumns in the nanostructure array is in range of about 150/100 μm 2 to about 1,000/100 μm 2 (the number of nanocolumns/area of nanostructure array). 12 . A method of forming a spheroid of an induced pluripotent stem cell, the method comprising: culturing an induced pluripotent stem cell on a nanostructure array. 13 . The method of claim 12 , wherein the nanostructure array has a plurality of nanocolumns that are spaced at random intervals on a substrate. 14 . The method of claim 12 , wherein the length of each nanocolumn in the nanostructure array is in a range of about 0.5 μm to about 1.5 μm. 15 . The method of claim 12 , the density of nanocolumns in the nanostructure array is in range of about 150/100 μm 2 to about 1,000/100 μm 2 (the number of nanocolumns/area of nanostructure array).

Assignees

Inventors

Classifications

  • Microcarriers · CPC title

  • Supports or coatings for cell culture characterised by topography · CPC title

  • using microcarriers · CPC title

  • C12N5/0696Primary

    Artificially induced pluripotent stem cells, e.g. iPS · CPC title

  • Transcription factors · CPC title

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What does patent US2017191037A1 cover?
Provided are a method of culturing an induced pluripotent stem cell and a method of forming a spheroid of the induced pluripotent stem cell. According to an embodiment of the present inventive concept, the induced pluripotent stem cell may have improved stemness.
Who is the assignee on this patent?
Korea Inst Sci & Tech
What technology area does this patent fall under?
Primary CPC classification C12N5/0696. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Thu Jul 06 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).