Methods of developing plant somatic embryos

Patent metadata

Field	Value
Publication number	US-2017191028-A1
Application number	US-201615347628-A
Country	US
Kind code	A1
Filing date	Nov 9, 2016
Priority date	Dec 31, 2015
Publication date	Jul 6, 2017
Grant date	—

Abstract

Methods of developing plant somatic embryos include incubating the plant somatic embryos at a first temperature for a first period of time followed by storing the plant somatic embryos at a second temperature for a second period of time. The first temperature can be in the range of about 23° C. to 30° C. and leads to rapid plant somatic embryo growth. The second temperature can be in the range of about 8° C. to 15° C. and slows plant somatic embryo development. Plant somatic embryos developed in this manner and implanted in artificial seeds show germination rates similar to zygotic embryos implanted in artificial seeds.

First claim

I/We claim:

1 . A method of developing a plant somatic embryo, comprising:
(a) developing the plant somatic embryo at a temperature in the range of about 23° C. to about 30° C.; and (b) developing the plant somatic embryo at a temperature in the range of about 8° C. to 15° C.

2 . The method of claim 1 , wherein developing the plant somatic embryo at a temperature in the range of about 23° C. to about 30° C. comprises incubating the plant somatic embryo at a temperature in the range of about 23° C. to about 30° C. until the plant somatic embryo enters the cotyledon stage.

3 . The method of claim 1 , wherein developing the plant somatic embryo at a temperature in the range of about 23° C. to about 30° C. comprises incubating the plant somatic embryo at a temperature in the range of about 23° C. to about 30° C. in a first environment and developing the plant somatic embryo at a temperature in the range of about 8° C. to 15° C. comprises incubating the plant somatic embryo at a temperature in the range of about 8° C. to 15° C. in a second environment, and the method further comprises transferring the plant somatic embryo from the first environment to the second environment.

4 . The method of claim 1 , wherein the method further comprises:
plating the plant somatic embryo on a development plate having development media disposed thereon prior to developing the plant somatic embryo at a temperature in the range of about 23° C. to about 30° C.

5 . The method of claim 1 , wherein developing the plant somatic embryo at a temperature in the range of about 23° C. to about 30° C. comprises developing the plant somatic embryo at a temperature of about 25° C.

6 . The method of claim 5 , wherein developing the plant somatic embryo at a temperature of about 25° C. comprises developing the plant somatic embryo at a temperature of about 25° C. for a period of from 3 to 6 weeks.

7 . The method of claim 1 , wherein developing the plant somatic embryo at a temperature in the range of about 8° C. to about 15° C. comprises developing the plant somatic embryo at a temperature of about 12° C.

8 . A method of developing plant somatic embryos, comprising:
(a) developing the plant somatic embryos at a temperature of about 25° C. until a majority of the plant somatic embryos enter the cotyledon stage; and (b) developing the plant somatic embryos at a temperature of about 12° C.

9 . The method of claim 8 , wherein developing the plant somatic embryos at a temperature of about 25° C. comprises incubating the plant somatic embryos at a temperature of about 25° C. in a first environment and developing the plant somatic embryos at a temperature of about 12° C. comprises incubating the plant somatic embryos at a temperature of about 12° C. in a second environment, and the method further comprises transferring the plant somatic embryos from the first environment to the second environment.

10 . The method of claim 8 , wherein the method further comprises:
plating the plant somatic embryos on a development plate having development media disposed thereon prior to developing the plant somatic embryos at a temperature of about 25° C.

Assignees

• Weyerhaeuser Nr Co

Inventors

• Gupta Pramod K

Classifications

• C12N2523/00

  Culture process characterised by temperature · CPC title

• A01H4/006

  Encapsulated embryos for plant reproduction, e.g. artificial seeds · CPC title

• C12N5/04 Primary

  Plant cells or tissues {(culture media C12N5/0025)} · CPC title

• A01H4/005 Primary

  Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques · CPC title