Enzyme immobilized adhesive layer for analyte sensors

Patent metadata

Field	Value
Publication number	US-2017188902-A1
Application number	US-201615394650-A
Country	US
Kind code	A1
Filing date	Dec 29, 2016
Priority date	Dec 30, 2015
Publication date	Jul 6, 2017
Grant date	—

Abstract

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.

First claim

What is claimed is:

1 . A device for measurement of an analyte concentration, the device comprising:
a transcutaneous sensor configured to generate a signal associated with a concentration of an analyte; and a sensing membrane located over the sensor, the sensing membrane comprising an enzyme layer, wherein the enzyme layer comprises an enzyme and an enzyme-layer polymer, wherein the polymer comprises one or more zwitterionic repeating units.

2 . The device of claim 1 , wherein the enzyme-layer polymer further comprises polyurethane and/or polyurea segments.

3 . The device of claim 1 , wherein the one or more zwitterionic repeating units comprise a betaine unit.

4 . The device of claim 1 , wherein the zwitterionic repeating units comprise a first betaine unit and a second betaine unit, wherein the first betaine unit is different from the second betaine unit.

5 . The device of claim 1 , wherein the zwitterionic repeating units are not located at an end group.

6 . The device of claim 1 , wherein the enzyme-layer polymer has a molecular weight from 10 kDa to 500,000 kDa.

7 . The device of claim 1 , wherein the enzyme-layer polymer has a molecular weight from 10 kDa to 100,000 kDa.

8 . The device of claim 1 , wherein the enzyme-layer polymer has a molecular weight from 100,000 kDa to 500,000 kDa.

9 . The device of claim 1 , wherein the enzyme-layer polymer has a polydispersity index of from about 1.4 to about 3.5.

10 . The device of claim 1 , wherein the enzyme-layer polymer has a contact angle of from about 10° to about 90°.

11 . The device of claim 1 , wherein the one or more zwitterionic repeating units is at least about 1 wt. % based on the total weight of the polymer.

Assignees

• Dexcom Inc

Inventors

• Wang Shanger
• Lee Ted Tang
• Zou Jiong

Classifications

• A61K36/185

  Magnoliopsida (dicotyledons) · CPC title

• A61B5/14865

  invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors · CPC title

• C08G18/4018

  Mixtures of compounds of group C08G18/42 with compounds of group C08G18/48 · CPC title

• A61B5/14546

  for measuring analytes not otherwise provided for, e.g. ions, cytochromes · CPC title

• G01N27/3275

  Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction · CPC title