Method for the preparation of a cell culture insert with at least one membrane
US-12162212-B2 · Dec 10, 2024 · US
US2017173878A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2017173878-A1 |
| Application number | US-201615382565-A |
| Country | US |
| Kind code | A1 |
| Filing date | Dec 16, 2016 |
| Priority date | Dec 16, 2015 |
| Publication date | Jun 22, 2017 |
| Grant date | — |
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A printer fabricates an object from a computerized model using a fused filament fabrication process. The shape of an extrusion nozzle may be varied during extrusion to control, e.g., an amount of build material deposited, a shape of extrudate exiting the nozzle, a feature resolution, and the like.
Opening claim text (preview).
1 . A printer for three-dimensional fabrication, the printer comprising: a reservoir to receive a build material from a source, the build material having a working temperature range where the build material exhibits plastic behavior suitable for extrusion; a heating system operable to heat the build material within the reservoir to a temperature within the working temperature range; a nozzle including a variable opening that provides a path for the build material to exit the reservoir, the variable opening formed between a plate and die, wherein the plate includes an opening and wherein the die is configured to slide relative to the plate to adjust a portion of the opening exposed for extrusion; and a drive system operable to mechanically engage the build material at a temperature below the working temperature range and advance the build material from the source into the reservoir with sufficient force to extrude the build material, while at a temperature within the working temperature range, through the opening in the nozzle. 2 . The printer of claim 1 further comprising a controller configured to fully close the variable opening to terminate an extrusion of the build material. 3 . The printer of claim 1 further comprising a controller configured to adjust a size of the variable opening according to a target feature size for an object fabricated by the printer from the build material. 4 . The printer of claim 1 further comprising a controller configured to adjust a size of the variable opening to increase an extrusion cross section during fabrication of one or more interior structures for an object and to decrease the extrusion cross section during fabrication of one or more exterior structures for the object. 5 . The printer of claim 1 further comprising a controller configured to adjust a size of the variable opening to increase an extrusion cross section during fabrication of a support structure for an object and to decrease the extrusion cross section during fabrication of one or more exterior structures for the object. 6 . The printer of claim 1 wherein the opening in the plate includes a wedge. 7 . The printer of claim 1 further comprising a rotating mount rotationally coupling the nozzle to the printer and a rotating drive to control a rotational orientation of the nozzle during extrusion. 8 . The printer of claim 1 wherein the build material includes a thermoplastic. 9 . The printer of claim 1 wherein the build material includes a binder system loaded with a powdered metal build material. 10 . The printer of claim 1 wherein the build material includes a bulk metallic glass. 11 . The printer of claim 10 wherein the working temperature range includes a range of temperatures above a glass transition temperature for the bulk metallic glass and below a melting temperature for the bulk metallic glass. 12 . The printer of claim 1 wherein the build material includes a non-eutectic composition of eutectic systems that are not at a eutectic composition. 13 . The printer of claim 12 wherein the working temperature range includes a range of temperatures above a eutectic temperature for the non-eutectic composition and below a melting point for each component species of the non-eutectic composition. 14 . The printer of claim 1 wherein the build material includes a metallic base that melts at a first temperature and a high-temperature inert second phase in particle form that remains inert up to at least a second temperature greater than the first temperature. 15 . The printer of claim 14 wherein the working temperature range includes a range of temperatures above a melting point for the metallic base. 16 . The printer of claim 1 wherein the printer comprises a fused filament fabrication additive manufacturing system. 17 . The printer of claim 16 further comprising a build plate and a robotic system, the robotic system configured to move the nozzle in a three-dimensional path relative to the build plate in order to fabricate an object from the build material on the build plate according to a computerized model of the object. 18 . The printer of claim 17 further comprising a controller configured by computer executable code to control the heating system, the drive system, and the robotic system to fabricate the object on the build plate from the build material. 19 . The printer of claim 17 further comprising a build chamber housing at least the build plate and the nozzle, the build chamber maintaining a build environment suitable for fabricating an object on the build plate from the build material. 20 . The printer of claim 19 further comprising a vacuum pump coupled to the build chamber for creating a vacuum within the build environment. 21 - 29 . (canceled)
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