Ion generation using modified wetted porous materials

Patent metadata

Field	Value
Publication number	US-2017168032-A1
Application number	US-201615359031-A
Country	US
Kind code	A1
Filing date	Nov 22, 2016
Priority date	Jun 3, 2011
Publication date	Jun 15, 2017
Grant date	—

Abstract

The invention generally relates to ion generation using modified wetted porous materials. In certain aspects, the invention generally relates to systems and methods for ion generation using a wetted porous substrate that substantially prevents diffusion of sample into the substrate. In other aspects, the invention generally relate to ion generation using a wetted porous material and a drying agent. In other aspects, the invention generally relates to ion generation using a modified wetted porous substrate in which at least a portion of the porous substrate includes a material that modifies an interaction between a sample and the substrate.

First claim

1 . A system for analyzing a sample material comprising:
at least one porous substrate connected to a high voltage source, wherein the porous substrate comprises a drying agent; and a mass analyzer.

2 - 90 . (canceled)

91 . The system according to claim 1 , wherein the porous substrate is discrete from a flow of solvent.

92 . The system according to claim 1 , wherein the mass analyzer is for a mass spectrometer or a handheld mass spectrometer.

93 . The probe according to claim 1 , wherein the porous material is paper or PVDF membrane.

94 . The probe according to claim 93 , wherein the paper is filter paper.

95 . The probe according to claim 1 , wherein the drying agent is an anhydrous salt.

96 . The probe according to claim 1 , further comprising an internal standard.

97 . A method for analyzing a sample comprising:
contacting a sample to a porous substrate, wherein the porous substrate comprises a drying agent; applying a high voltage to the porous material to generate ions of an analyte in the sample that are expelled from the porous material; and analyzing the expelled ions.

98 . The method according to claim 97 , wherein the porous substrate is kept separate from a flow of solvent.

99 . The method according to claim 97 , further comprising applying a solvent to the porous material.

100 . The method according to claim 99 , wherein the solvent assists in transport of the sample through the porous material.

101 . The method according to claim 99 , wherein the solvent comprises an internal standard.

102 . The method according to claim 99 , wherein the solvent minimizes salt and matrix effects.

103 . The method according to claim 99 , wherein the solvent is capable of mixing with the sample.

104 . The method according to claim 97 , wherein prior to applying the high voltage, the method further comprises drying the substrate.

105 . The method according to claim 97 , wherein analyzing comprises providing a mass analyzer to generate a mass spectrum of analytes in the sample.

106 . The method according to claim 97 , wherein the sample is a biological fluid.

107 . The method according to claim 106 , wherein the biological fluid is blood.

108 . A method for analyzing blood, the method comprising:
contacting a blood sample to a porous substrate, wherein the porous substrate comprises a drying agent; drying the blood sample on the substrate; applying a solvent to the substrate; applying a high voltage to the substrate to generate ions of the components that are expelled from the porous substrate; and analyzing the expelled ions.

109 . The method according to claim 108 , wherein applying the solvent and the high voltage occur simultaneously.

110 . The method according to claim 108 , wherein applying the solvent and the high voltage occur sequentially.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Ouyang Zheng
• Wang He

Classifications

• H01J49/168 Primary

  field ionisation, e.g. corona discharge (atmospheric pressure corona discharge per se H01T19/00) · CPC title

• G01N33/49

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G01N30/91

  Application of the sample · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• G01N30/95 Primary

  Detectors specially adapted therefor; Signal analysis · CPC title