Pipette tip for electroporation device

Patent metadata

Field	Value
Publication number	US-2017166854-A1
Application number	US-201615357985-A
Country	US
Kind code	A1
Filing date	Nov 21, 2016
Priority date	Apr 15, 2008
Publication date	Jun 15, 2017
Grant date	—

Abstract

Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.

First claim

1 .- 29 . (canceled)

30 . A system for electroporation of a sample, the system comprising:
a pulse generator; a pipette; a pipette tip configured to be mounted on the pipette, wherein the pipette tip includes a void and a conductor, wherein the void is configured to hold a sample, wherein the conductor is in electrical communication with the pulse generator and is configured to apply an electrical pulse to the sample within the void.

31 . The system of claim 30 , wherein the conductor is located at least partially on or within an outer surface of the pipette tip.

32 . The system of claim 31 , wherein the conductor is ring-shaped.

33 . The system of claim 31 , wherein the pipette tip includes a post, and wherein the conductor surrounds at least a portion of the post.

34 . The system of claim 33 , wherein the post is funnel shaped.

35 . The system of claim 31 , wherein the pipette tip comprises a plurality of conductors.

36 . The system of claim 35 , wherein the plurality of conductors is disposed in an equiangular set of positions.

37 . The system of claim 36 , wherein the pipette tip includes a plurality of posts, wherein the pipette tip includes a plurality of conductors, and wherein each of the plurality of conductors surrounds at least a portion of each of the plurality of posts.

38 . The system of claim 37 , wherein the pipette tip comprises a combining part located at a proximal end of the pipette tip and is configured to mount on the pipette, an elongated part located at a distal end of the pipette tip, and a connecting part located between the combining part and the elongated part.

39 . The system of claim 38 , wherein the connecting part includes the plurality of posts.

40 . The system of claim 39 , wherein the plurality of posts is disposed in an equiangular set of positions.

41 . The system of claim 40 , wherein the plurality of posts comprises three posts, and wherein each post is disposed at an angle of 120° respective to other posts.

42 . The system of claim 38 , wherein the void comprises a first void within the elongated part, a second void within the connecting part and a third void within the combining part, and wherein the first, second and third voids are in fluid communication.

43 . The system of claim 30 , wherein the pipette tip does not include an electrode within the void.

44 . The system of claim 30 , wherein the pipette tip does not include a plunger.

45 . The system of claim 30 , wherein the pipette and pipette tip are configured to load the sample into the void and remove the sample from the void only using air pressure.

46 . The system of claim 30 , wherein the conductor comprises a conductive material selected from the group consisting of: nickel, gold, silver, copper, brass and aluminum.

47 . The system of claim 30 , wherein the conductor comprises a conductive polymer.

Assignees

• Life Technologies Corp

Inventors

• Chang Jun-Keun
• Cho Keun-Chang
• Chung Chan-Il
• Jung Neon-Cheol
• Huh Seung Gin

Classifications

• B01L3/0237

  Details of electronic control, e.g. relating to user interface · CPC title

• C12M35/02 Primary

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title

• C12M33/04

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title

• B01L2300/16

  Surface properties and coatings · CPC title