Processing biomass
US-2015353974-A1 · Dec 10, 2015 · US
US2017101655A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2017101655-A1 |
| Application number | US-201514901532-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jun 22, 2015 |
| Priority date | Jun 23, 2014 |
| Publication date | Apr 13, 2017 |
| Grant date | — |
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Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.
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1 . A microorganism of Escherichia sp. producing O-acetyl homoserine, wherein the endogenous activity of citrate synthase is attenuated or inactivated. 2 . The microorganism of Escherichia sp. according to claim 1 , wherein the microorganism with the attenuated endogenous activity of citrate synthase has an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2. 3 . The microorganism of Escherichia sp. according to claim 1 , wherein the activity of cystathionine gamma synthase, homoserine kinase, or both is further attenuated or inactivated compared to their endogenous activities. 4 . The microorganism of Escherichia sp. according to claim 1 , wherein the activity of homoserine O-acetyltransferase is further introduced or enhanced, or the endogenous homoserine O-succinyltransferase is further modified to have the activity of homoserine O-acetyltransferase. 5 . The microorganism of Escherichia sp. according to claim 1 , wherein the activity of at least one protein selected from the group consisting of phosphoenolpyruvate carboxylase, aspartate aminotransferase, and aspartate semialdehyde dehydrogenase is further introduced or enhanced. 6 . The microorganism of Escherichia sp. according to claim 1 , wherein the microorganism is Escherichia coli. 7 . A method for producing O-acetyl homoserine, comprising: (a) culturing the microorganism of any of claims 1 to 6 ; and (b) recovering O-acetyl homoserine produced during the cultivation of the microorganism.
Genes encoding for enzymes or proenzymes · CPC title
transferring groups other than amino-acyl groups (2.3.1) · CPC title
Lyases (4.) · CPC title
Homoserine kinase (2.7.1.39) · CPC title
Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title
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