Microorganism producing o-acetyl homoserine and the method of producing o-acetyl homoserine using the same

US2017101655A1 · US · A1

Patent metadata
FieldValue
Publication numberUS-2017101655-A1
Application numberUS-201514901532-A
CountryUS
Kind codeA1
Filing dateJun 22, 2015
Priority dateJun 23, 2014
Publication dateApr 13, 2017
Grant date

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  1. Title

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  2. Abstract

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  5. First independent claim

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Abstract

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Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.

First claim

Opening claim text (preview).

1 . A microorganism of Escherichia sp. producing O-acetyl homoserine, wherein the endogenous activity of citrate synthase is attenuated or inactivated. 2 . The microorganism of Escherichia sp. according to claim 1 , wherein the microorganism with the attenuated endogenous activity of citrate synthase has an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2. 3 . The microorganism of Escherichia sp. according to claim 1 , wherein the activity of cystathionine gamma synthase, homoserine kinase, or both is further attenuated or inactivated compared to their endogenous activities. 4 . The microorganism of Escherichia sp. according to claim 1 , wherein the activity of homoserine O-acetyltransferase is further introduced or enhanced, or the endogenous homoserine O-succinyltransferase is further modified to have the activity of homoserine O-acetyltransferase. 5 . The microorganism of Escherichia sp. according to claim 1 , wherein the activity of at least one protein selected from the group consisting of phosphoenolpyruvate carboxylase, aspartate aminotransferase, and aspartate semialdehyde dehydrogenase is further introduced or enhanced. 6 . The microorganism of Escherichia sp. according to claim 1 , wherein the microorganism is Escherichia coli. 7 . A method for producing O-acetyl homoserine, comprising: (a) culturing the microorganism of any of claims 1 to 6 ; and (b) recovering O-acetyl homoserine produced during the cultivation of the microorganism.

Assignees

Inventors

Classifications

  • Genes encoding for enzymes or proenzymes · CPC title

  • transferring groups other than amino-acyl groups (2.3.1) · CPC title

  • Lyases (4.) · CPC title

  • Homoserine kinase (2.7.1.39) · CPC title

  • Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title

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What does patent US2017101655A1 cover?
Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.
Who is the assignee on this patent?
Cj Cheiljedang Corp
What technology area does this patent fall under?
Primary CPC classification C12P13/06. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Thu Apr 13 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).