Microfluidic Device For Real-Time Clinical Monitoring And Quantitative Assessment Of Whole Blood Coagulation

Patent metadata

Field	Value
Publication number	US-2017100714-A1
Application number	US-201615393596-A
Country	US
Kind code	A1
Filing date	Dec 29, 2016
Priority date	Oct 16, 2013
Publication date	Apr 13, 2017
Grant date	—

Abstract

A microfluidic coagulation assessment device includes a plurality of microchannels, with a blood sample driven through the microchannels at a substantially constant flow rate. A controller is configured to, in combination with a timer and a pressure sensing device, determine a first pressure value (or flow value) at an initiation of flow, a first time (T pg ) at which a second pressure value is about twice the determined first pressure value, and a second time (T pf ) at which a third pressure value is about (1+e) times the determined first pressure value and establish a subject coagulation model predictive of channel occlusion therefrom.

First claim

1 . A microfluidic coagulation device, comprising:
at least one substrate defining a plurality of microchannels; a first port at a first end portion of the substrate, the first port connecting to first ends of the plurality of microchannels; a second port at a second end portion of the substrate, the second port connecting to second ends of the plurality of microchannels; a first sensing device configured to determine a pressure value in, or relating to, a pressure across the plurality of microchannels; a timer; and a controller configured to determine, in combination with the first sensing device and the timer, a first pressure value at an initiation of flow, a first time at which a second pressure value is determined to be about twice the determined first pressure value, and a second time at which a third pressure value is about (1+e) times the determined first pressure value, and further configured to establish a subject coagulation model predictive of channel occlusion in accord with the relation

Δ




P


(
t
)

Δ




P


(
0
)

=

1
+



t
-

T
pg

T
pf

-

T
pg

wherein T pf is the second time and T pg is the first time, and
wherein a differential pressure or flow rate/shear applied across the first port drives a blood sample across the plurality of microchannels at a substantially constant flow rate.

2 - 86 . (canceled)

Assignees

• Harvard College

Inventors

• Jain Abhishek
• Waterhouse Anna
• Super Mike
• Ingber Donald E
• Leslie Daniel C

Classifications

• B01L2300/0861

  Configuration of multiple channels and/or chambers in a single devices · CPC title

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title

• G01N33/86

  involving blood coagulating time {or factors, or their receptors} · CPC title

• B01L3/50273 Primary

  characterised by the means or forces applied to move the fluids · CPC title

• G01N33/4905

  Determining clotting time of blood (by chemical methods G01N33/86, C12Q1/56) · CPC title