Mesoporous catalysts of magnetic nanoparticles and free-radicalproducing enzymes, and methods of use

Patent metadata

Field	Value
Publication number	US-2017096658-A1
Application number	US-201615380104-A
Country	US
Kind code	A1
Filing date	Dec 15, 2016
Priority date	Mar 10, 2011
Publication date	Apr 6, 2017
Grant date	—

Abstract

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

First claim

1 .- 47 . (canceled)

48 . A composition comprising lactoperoxidase immobilized within mesoporous aggregates of magnetic nanoparticles, wherein said mesoporous aggregates of magnetic nanoparticles have mesopores in which said lactoperoxidase is embedded, wherein said mesopores are located between magnetic nanoparticles in said aggregates of magnetic nanoparticles, and wherein said mesopores and aggregates of magnetic nanoparticles in said mesoporous aggregates are simultaneously produced by a self-assembly process in which said lactoperoxidase and magnetic nanoparticles are combined in solution.

49 . The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles have an iron oxide composition.

50 . The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 90% of magnetic nanoparticles have a size of at least 3 nm and up to 30 nm, and an aggregated particle size distribution in which at least 90% of said mesoporous aggregates of magnetic nanoparticles have a size of at least 10 nm and up to 500 nm.

51 . The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles possess a saturated magnetization of at least 10 emu/g.

52 . The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles possess a remanent magnetization up to 5 emu/g.

53 . The composition of claim 1 , wherein said lactoperoxidase is contained in said mesoporous aggregates of magnetic nanoparticles in up to 100% of saturation capacity.

54 . The composition of claim 1 , wherein said mesopores are characterized by a pore size distribution in which at least 90% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

55 . The composition of claim 1 , wherein said mesopores are characterized by a pore size distribution in which at least 95% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

56 . The composition of claim 1 , further comprising an oxidase.

57 . The composition of claim 9 , wherein said oxidase is glucose oxidase.

58 . The composition of claim 1 , wherein said mesoporous aggregates of magnetic nanoparticles and lactoperoxidase reside within submicrometric particles having a size of at least 20 nanometers.

59 . A composition comprising an enzyme immobilized within mesoporous aggregates of magnetic nanoparticles, wherein said mesoporous aggregates of magnetic nanoparticles have mesopores in which said enzyme is embedded, wherein said mesopores are located between magnetic nanoparticles in said aggregates of magnetic nanoparticles, and wherein said mesopores and aggregates of magnetic nanoparticles in said mesoporous aggregates are simultaneously produced by a self-assembly process in which said enzyme and magnetic nanoparticles are combined in solution, wherein said enzyme is selected from the group consisting of EC 1.1.2, EC 1.10.2, EC 1.14 oxidoreductase, cytochrome-b5 reductase, cytochrome-c reductase, and cytochrome-c peroxidase.

60 . The composition of claim 12 , wherein said mesoporous aggregates of magnetic nanoparticles have an iron oxide composition.

61 . The composition of claim 12 , wherein said mesoporous aggregates of magnetic nanoparticles have a magnetic nanoparticle size distribution in which at least 90% of magnetic nanoparticles have a size of at least 3 nm and up to 30 nm, and an aggregated particle size distribution in which at least 90% of said mesoporous aggregates of magnetic nanoparticles have a size of at least 10 nm and up to 500 nm.

62 . The composition of claim 12 , wherein said mesoporous aggregates of magnetic nanoparticles possess a saturated magnetization of at least 10 emu/g.

63 . The composition of claim 12 , wherein said mesoporous aggregates of magnetic nanoparticles possess a remnant magnetization up to 5 emu/g.

64 . The composition of claim 12 , wherein said enzyme is contained in said mesoporous aggregates of magnetic nanoparticles in up to 100% of saturation capacity.

65 . The composition of claim 12 , wherein said mesopores are characterized by a pore size distribution in which at least 95% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

66 . The composition of claim 12 , wherein said mesopores are characterized by a pore size distribution in which at least 90% of said pore volume is attributed to pores having a pore size of at least 2 nm and up to 20 nm.

Assignees

• Univ Cornell

Inventors

• Corgie Stephane C
• Kahawong Patarawan
• Giannelis Emmanuel P
• Walker Larry P

Classifications

• C12Y111/01014

  Lignin peroxidase (1.11.1.14) · CPC title

• C12N9/0065

  acting on hydrogen peroxide as acceptor (1.11) · CPC title

• C12Y111/01005

  Cytochrome-c peroxidase (1.11.1.5) · CPC title

• C12Y111/01013

  Manganese peroxidase (1.11.1.13) · CPC title

• C12Y101/03004

  Glucose oxidase (1.1.3.4) · CPC title