Automatic gas valve container holder for chemical synthesis

Patent metadata

Field	Value
Publication number	US-2017066798-A1
Application number	US-201515122609-A
Country	US
Kind code	A1
Filing date	Mar 12, 2015
Priority date	Mar 12, 2014
Publication date	Mar 9, 2017
Grant date	—

Abstract

The invention relates to a container holder 10, comprising a main body 12 which in turn comprises a gas inlet 16; a solution liquid outlet 18; a gas control valve 20 through which a gas enters the container 100 from the gas inlet; and a sealing means 22 for the container, which sealing means includes a passageway 24 for the input of gas and output of a solution in the container via an egress tube 19; wherein when the container is connected to the container holder through the sealing means, the gas control valve opens automatically, and when the container is disconnected the gas control valve is closed automatically. The invention further relates to a container panel 50 which includes two or more container holders. Also disclosed are methods of using these containers and container panels for synthesizing a polypeptide.

First claim

1 . A container holder, comprising a main body which in turn comprises:
(a) a gas inlet; (b) a solution outlet; (c) a gas control valve which allows a gas to enter the container from the gas inlet; and (d) a sealing means for sealing the container in use, which sealing means includes a passageway for the input of the gas and for the egress of a liquid in the container; wherein, when the container is sealingly connected to the container holder by the sealing means, the gas control valve opens automatically, and when the container is disconnected from the container holder the gas control valve is closed automatically.

2 . The container holder of claim 1 , wherein the gas control valve is a spring valve operable by means of a plate, in turn displaced be the act of sealingly connecting the container to the container holder.

3 . The container holder of claim 1 , wherein the main body includes an internally threaded aperture and wherein the sealing means comprises a threaded part complementary to the threaded aperture and a plate, wherein when the container is connected with the threaded part, the plate opens the gas control valve.

4 . The container holder of claim 2 , wherein the plate includes a passage for fluidically interconnecting the container and the inlet, and the outlet includes an egress tube for extended into the container, said passage accommodating said tube.

5 . The container holder of claim 3 , further comprising a flat seal under the disc, and an O-ring between the disc and an inside surface of the aperture.

6 . The container holder of claim 3 , further comprising one or more guide pins operable with the plate to prevent the plate from rotating.

7 . The container holder of claim 6 , further comprising a coil spring for urging the guide pin into an operable position.

8 . The container holder of claim 1 , wherein at least some of the containers are 50 ml tubes.

9 . The container holder of claim 1 , wherein when the gas control valve is in an open position, the container is pressurizable with an inert gas and the solution in the container is insulated from its external environment.

10 . The container holder of claim 9 , wherein the inert gas is moisture-free.

11 . The container holder of claim 9 , wherein the inert gas is nitrogen.

12 . The container holder of claim 1 , for use to supply an amino acid solution to a synthesizer such as a peptide or oligonucleotide synthesizer.

13 . A container holder panel, comprising two or more container holders of claim 1 .

14 . The container holder panel of claim 13 , comprising at least two rows of container holders, each row comprising at least two container holders.

15 . The container holder panel of claim 13 , comprising four rows of container holders, each row including 8 container holders.

16 . The container holder panel of claim 13 , wherein the gas inlets of each container holder are connected to a common gas source.

17 . The container holder panel of claim 13 , for use to supply an amino acid solution to a peptide synthesizer.

18 . The container holder panel of claim 13 , for use to supply all amino acid solutions to a peptide synthesizer.

19 . A method for synthesizing a polypeptide, comprising a cycling of synthesis steps:
(a) de-protection of alpha-amino protecting group on existing peptide; (b) pre-activation of next amino acid in a mixer from a supply of amino acid; and (c) coupling of activated amino acid to the existing peptide;
wherein at least one amino acid is supplied through a container connected to the container holder of claim 1 or the container holder panel.

20 . The method of claim 19 , wherein the or each amino acid is supplied through a respective container connected to a container holder or container holder panel.

21 . A method for synthesizing a polypeptide, comprising a cycling of synthesis steps:
(a) de-protection of alpha-amino protecting group on existing peptide; (b) pre-activation of next amino acid in a mixer from a supply of amino acid; and (c) coupling of activated amino acid to the existing peptide;
wherein at least one amino acid is supplied through a container connected to a container holder of the container holder panel of claim 13 .

22 . The method of claim 17 , wherein each amino acid is supplied through a container connected to a container holder of the container holder panel.

Assignees

• Ge Healthcare Bio Sciences Ab

Inventors

• Olovsson Bjorn Markus
• Osmark Mathias
• Gamberg Mangus
• Carlsson Daniel

Classifications

• B01J2219/00308

  interchangeably mounted in racks or blocks · CPC title

• B01J2219/00337

  Valves · CPC title

• C07K1/00

  General methods for the preparation of peptides {, i.e. processes for the organic chemical preparation of peptides or proteins of any length} · CPC title

• C07K1/045 Primary

  using devices to improve synthesis, e.g. reactors, special vessels · CPC title

• B65D83/44

  Valves specially adapted for the discharge of contents; Regulating devices (pressure relief devices B65D83/70) · CPC title