Method for preparing water extract of ashwagandha leaves which has enhanced anti-cancer activity utilizing cyclodextrin, and pharmaceutical composition containing ashwagandha leaves

Patent metadata

Field	Value
Publication number	US-2017007659-A1
Application number	US-201515115345-A
Country	US
Kind code	A1
Filing date	Jan 29, 2015
Priority date	Jan 29, 2014
Publication date	Jan 12, 2017
Grant date	—

Abstract

It is an object of the present invention to provide a method for preparing a water extract of ashwagandha, which comprises increasing the amounts of withanone and withaferin A contained as active ingredients in the water extract of ashwagandha leaves. In addition, it is another object of the present invention to more economically and simply provide a pharmaceutical composition comprising ashwagandha leaves.
The present invention relates to a method for preparing a water extract of ashwagandha leaves, which comprises extracting ashwagandha leaves with water in the presence of cyclodextrin, and a method for enhancing the anticancer activity of the water extract of ashwagandha leaves. The present invention also relates to a pharmaceutical composition for treating or preventing cancer, comprising the water extract of ashwagandha leaves. The present invention further relates to a pharmaceutical composition comprising dry powders of ashwagandha leaves and cyclodextrin.

First claim

1 . A method for preparing a water extract of ashwagandha leaves, which comprises extracting ashwagandha leaves with water in the presence of cyclodextrin.

2 . The preparation method according to claim 1 , wherein the cyclodextrin is at least one selected from the group consisting of α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, hydroxypropyl-β-cyclodextrin, sulfobutyl ether-β-cyclodextrin, and branched cyclodextrin.

3 . A method for enhancing the anticancer activity of a water extract of ashwagandha leaves, using cyclodextrin.

4 . (canceled)

5 . (canceled)

6 . (canceled)

7 . (canceled)

8 . (canceled)

9 . A method for treating or preventing cancer, which comprises administrating the water extract of ashwagandha leaves obtained by the preparation method according to claim 1 to a subject in need thereof.

10 . A method for treating or preventing cancer, which comprises administrating a composition obtained by mixing a water extract of ashwagandha leaves with cyclodextrin or administrating dry powders of ashwagandha leaves and cyclodextrin simultaneously, separately or sequentially to a subject in need thereof.

11 . The method according to claim 10 , wherein the cyclodextrin is γ-cyclodextrin.

Assignees

• Nat Inst Advanced Ind Science & Tech
• Cyclochem Co Ltd

Inventors

• Wadhwa Renu
• Kaul Sunil
• Terao Keiji
• Ishida Yoshiyuki

Classifications

• A61K9/08

  Solutions {(composition of solutions A61K47/00)} · CPC title

• A61K2236/53

  Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization · CPC title

• A61K9/16

  Agglomerates; Granulates; Microbeadlets {; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction (A61K9/20 takes precedence if the final form is a tablet; microspheres with drug-free outer coating, microcapsules A61K9/50; mixture of different granules, microcapsules, (coated) microparticles A61K9/5084; nanoparticles A61K9/51)} · CPC title

• A61K47/40

  Cyclodextrins; Derivatives thereof · CPC title

• A61K2236/331

  using water, e.g. cold water, infusion, tea, steam distillation or decoction · CPC title