Strategy for sucrose reduction and generation of insoluble fiber in juices

Patent metadata

Field	Value
Publication number	US-2017006902-A1
Application number	US-201515112674-A
Country	US
Kind code	A1
Filing date	Feb 4, 2015
Priority date	Feb 13, 2014
Publication date	Jan 12, 2017
Grant date	—

Abstract

The present teachings provide a method of making a lower calorie, higher insoluble fiber beverage comprising; treating a sucrose-containing beverage with a glucosyltransferase to convert sucrose to alpha (1-3) glucan to make the lower calorie, higher insoluble fiber beverage. Additional methods, as well as compositions, are provided.

First claim

1 . A method of making a lower calorie, higher insoluble fiber beverage comprising;
treating a sucrose-containing beverage with a glucosyltransferase to convert sucrose to alpha (1-3) glucan to make the lower calorie, higher insoluble fiber beverage.

2 . The method of claim 1 ,
further comprising, removing the alpha (1-3) glucan to make a lower calorie, clarified beverage.

3 . The method of claim 1 , wherein the lower calorie, higher insoluble fiber beverage contains no less than 95%, 96%, 97%, 98%, 99%, or 99.9% fructose.

4 . The method according to claim 1 wherein the treating comprises treating with a glucosyltransferase comprising SEQ ID NO: 1.

5 . The method according to claim 1 wherein the treating comprises treating with an enzyme 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99%, or 99.9% identical to SEQ ID NO:1.

6 . The method according to claim 1 wherein the treating comprises treating with an immobilized glucosyltransferase.

7 . The method according to claim 1 wherein the treating comprises treating with an immobilized glucosyltransferase from Streptococcus salivarius.

8 . The method according to claim 1 wherein the beverage comprises a fruit juice.

9 . The method according to claim 1 wherein the beverage comprises apple juice or orange juice.

10 . The method according to claim 1 wherein the beverage comprises a sucrose level reduced at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, or at least 90% compared to a control beverage lacking the treating.

11 . A beverage comprising at least 2, at least 2.5, at least 3, at least 4, or at least 4.3 grams of alpha (1-3) glucans per liter.

12 . The beverage of claim 11 , wherein the beverage is orange juice or apple juice, and wherein it contains no less than 95%, 96%, 97%, 98%, 99%, or 99.9% fructose of average orange juice or apple juice.

13 . A composition comprising an immobilized glucosyltransferase, and, a fruit juice.

14 . The composition of claim 13 wherein the glucosyltransferase is has an amino acid sequence having 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99%, 99.9%, or 100% amino acid sequence identity to SEQ ID NO: 1.

15 . The composition according to claim 13 wherein the fruit juice is apple juice or orange juice.

16 . (canceled)

Assignees

• Danisco Us Inc

Inventors

• Garske Adam L

Classifications

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title

• A23L2/66

  Proteins · CPC title

• A23L33/21

  Addition of substantially indigestible substances, e.g. dietary fibres · CPC title

• C12P19/04

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• A23L2/84 Primary

  using microorganisms or biological material, e.g. enzymes · CPC title